Pe cy5 conjugated b220

Manufactured by BD

Sourced in Germany

PE-Cy5-conjugated B220 is a fluorescently labeled antibody used for the detection and identification of B220-positive cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti mouse pacific blue conjugated cd11b, by Thermo Fisher Scientific (4 mentions) Lsrii flow cytometer, by BD (2 mentions) Fortessa flow cytometer, by BD (2 mentions) Allophycocyanin conjugated cd11c, by BD (2 mentions) Apc conjugated anti mouse epcam, by Thermo Fisher Scientific (1 mentions) Apc conjugated cd11c, by BD (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Igm pe, by Southern Biotech (1 mentions) Anti cd43 magnetic beads, by Miltenyi Biotec (1 mentions) Apc tcrβ clone h57 597, by BD (1 mentions) Anti cd40, by BD (1 mentions) Fitc conjugated igg1, by BD (1 mentions) 24 well plate, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Cd11b, by BD (1 mentions) Eflour450, by Thermo Fisher Scientific (1 mentions) Pe conjugated cd69, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

B220-PE (26 citations)

5 protocols using pe cy5 conjugated b220

Characterization of LA-4 Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols

For characterisation of LA-4 cells, the following antibodies were used: PE-conjugated anti-mouse TLR5 (clone: 85B152.5, 1:100, Abcam, Cambridge, UK), PE-conjugated anti-mouse MHCI (clone: 31-1-2S, 1:100, eBiosciences, Frankfurt, Germany), AF488-conjugated anti-mouse Pan-cytokeratin (clone: AE1/AE3, 1:50, eBiosciences), AF488-conjugated anti-human mucin 1 (clone: SM3, 1:25, eBiosciences), APC-conjugated anti-mouse EpCAM (clone: G8.8, 1:50, eBiosciences), and APC-conjugated anti-mouse CEACAM1 (clone: CC1, 1:50, eBiosciences). Bone marrow-derived mDCs were characterized using anti-mouse pacific blue-conjugated CD11b (clone: M1/70.15, 1:50, Invitrogen, ThermoFisher Scientific, Dreieich, Germany), APC-conjugated CD11c (clone: HL3, dilution: 1:500, BD Bioscience), and PE-Cy5-conjugated B220 (clone: RA3-6B2, dilution: 1:100, BD Bioscience), anti-mouse FITC-conjugated CD3 antibody (clone: 145-2C11, 1:50, BD Biosciences), anti-mouse FITC-conjugated CD19 antibody (clone: 6D5, 1:50, Southern Biotech), and anti-mouse FITC-conjugated CD49b pan NK cell antibody (clone: Dx5, 1:100, BioLegend). Cells were analyzed by flow cytometry using a LSR II flow cytometer (BD Bioscience). Data were analyzed using FlowJo either V.7 or V.10 (Treestar Inc., Ashland, OR, USA).

Lin Y.J., Flaczyk A., Wolfheimer S., Goretzki A., Jamin A., Wangorsch A., Vieths S., Scheurer S, & Schülke S. (2021). The Fusion Protein rFlaA:Betv1 Modulates DC Responses by a p38-MAPK and COX2-Dependent Secretion of PGE2 from Epithelial Cells. Cells, 10(12), 3415.

+ Open protocol

+ Expand

Lymphocyte Development and CSR Analysis

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lymphocyte development and in vitro CSR were performed as described before (10 (link),31 (link),32 ). Hematopoietic cells from 5–8 week mice were stained and analyzed on a FACS Calibur flow cytometer (BD Biosciences). The antibodies are: PE-CD4 (clone GK1.5, BD Pharmingen, 553730), FITC-CD8α (clone 53–6.7, BioLegend, 100705), APC-TCRβ (clone H57-597, BD Pharmingen, 553174), PE/Cy7 TER-119 (clone TER-119, BioLegend, 116222), FITC-CD43 (clone S7, BD Pharmingen, 553270), PE-Cy5-B220 (clone RA3-6B2, BD Pharmingen, 553091) and PE-IgM (Southern Biotech, 1020-09). For in vitro CSR, CD43⁻ splenic cells (anti-CD43 magnetic beads, Miltenyi, 130-049-801) were cultured (~1 × 10⁶ cells ml⁻¹) in RPMI (Gibco, 11875-093), serum supplements (10 (link),31 (link),32 ), IL-4 (20 ng/mL; R&D, 404-ML-050), and anti-CD40 (1 μg/mL; BD Bioscience, 553721) and analyses with FITC-conjugated IgG1 (clone A85-1, BD Pharmingen, 553443) and PECy5-conjugated B220 (clone RA3-6B2, BD Pharmingen, 553091). FlowJo software package was used for data analyses

Milanovic M., Houghton L.M., Menolfi D., Lee J.H., Yamamoto K., Li Y., Lee B.J., Xu J., Estes V.M., Wang D., Mckinnon P.J., Paull T.T, & Zha S. (2020). The cancer-associated ATM R3008H mutation reveals the link between ATM activation and its exchange. Cancer research, 81(2), 426-437.

+ Open protocol

+ Expand

Activation of Dendritic Cells by Allergens

Check if the same lab product or an alternative is used in the 5 most similar protocols

BALB/c or C57Bl/6 mDCs were seeded at 3.2 × 10⁵ cells/ml in 24-well plates (Thermo Scientific, Dreieich, Germany) and stimulated with the indicated equimolar concentrations of rFlaA, rArt v 1, rFlaA + rArt v 1, rFlaA:Artv1, or rFlaA:Artv1^hyp for 24 h. 10 µg/ml LPS (#L5886, Sigma Aldrich, Taufkirchen, Germany) served as positive control. Supernatants were analyzed for cytokine secretion by ELISA. The activation of mDCs was assessed by FACS using anti-mouse FITC-conjugated CD40 and phycoerythrin (PE)-conjugated CD69 mAbs (eBioscience, Frankfurt, Germany). Additionally, cells were stained with anti-mouse pacific blue-conjugated CD11b (Invitrogen, Thermo Fisher Scientific), allophycocyanin (APC)-conjugated CD11c (BD Bioscience, Heidelberg, Germany), and PE-Cy5-conjugated B220 (BD Bioscience) with their respective isotype controls. FITC or PE intensity of CD11b⁺CD11c⁺B220⁻ (mDC) cells was quantified by FACS using a LSR II flow cytometer (BD Bioscience, Heidelberg, Germany). For analysis of cell viability mDC were treated as indicated and stained for dead cells using the fixable viability dye eFlour450 (eBiosciences, Frankfurt, Germany). Data were analyzed using FlowJo 10 (Treestar Inc., Ashland, OR, USA).

Schülke S., Kuttich K., Wolfheimer S., Duschek N., Wangorsch A., Reuter A., Briza P., Pablos I., Gadermaier G., Ferreira F., Vieths S., Toda M, & Scheurer S. (2017). Conjugation of wildtype and hypoallergenic mugwort allergen Art v 1 to flagellin induces IL-10-DC and suppresses allergen-specific TH2-responses in vivo. Scientific Reports, 7, 11782.

+ Open protocol

+ Expand

Quantifying Murine Dendritic Cell Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The activation of mDCs was assessed by flow cytometry using anti-mouse PE-conjugated CD40 (clone: 1C10, dilution: 1 to 100), PE-conjugated CD69 (clone: H1.2F3, dilution: 1 to 150), FITC-conjugated CD80 (clone: 16-10A1, dilution: 1 to 50), FITC-conjugated CD86 (clone: GL1, dilution: 1 to 50, all eBiosciences, Frankfurt, Germany), as well as respective isotype controls. Additionally, cells were stained with anti-mouse pacific blue-conjugated CD11b (clone: M1/70.15, dilution: 1 to 50, Invitrogen, ThermoFisher Scientific), allophycocyanin-conjugated CD11c (clone: HL3, dilution: 1 to 500, BD Bioscience), and PE-Cy5-conjugated B220 (clone: RA3-6B2, dilution: 1 to 100, BD Bioscience) with their respective isotype controls. FITC or PE intensity of CD11b+CD11c+B220− (mDC) cells was quantified by FACS using a FORTESSA flow cytometer (BD Bioscience). Data were analyzed using FlowJo V.7 (Treestar Inc., Ashland, OR, USA).

Zimmermann J., Goretzki A., Meier C., Wolfheimer S., Lin Y.J., Rainer H., Krause M., Wedel S., Spies G., Führer F., Vieths S., Scheurer S, & Schülke S. (2022). Modulation of dendritic cell metabolism by an MPLA-adjuvanted allergen product for specific immunotherapy. Frontiers in Immunology, 13, 916491.

+ Open protocol

+ Expand

Flow Cytometry Analysis of rFlaA:Betv1-induced mDC Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

rFlaA:Betv1-induced mDC activation was analyzed by flow cytometry using anti-mouse PE-conjugated CD40 (clone: 1C10, dilution: 1 to 100), PE-conjugated CD69 (clone: H1.2F3, dilution: 1 to 150), FITC-conjugated CD80 (clone: 16–10A1, dilution: 1 to 50, all eBiosciences, Frankfurt, Germany). Additionally, cells were stained with anti-mouse pacific blue-conjugated CD11b (clone: M1/70.15, dilution: 1 to 50, Invitrogen, Thermo Fisher Scientific), allophycocyanin-conjugated CD11c (clone: HL3, dilution: 1 to 500, BD Bioscience), and PE-Cy5-conjugated B220 (clone: RA3–6B2, dilution: 1 to 100, BD Bioscience). The FITC- or PE-intensity of CD11b⁺CD11c⁺B220⁻ cells (mDCs) was quantified by flow cytometry using a FORTESSA flow cytometer (BD Bioscience). Geometrical mean fluorescence intensities were calculated and normalized to unstimulated cells. Data were analyzed using FlowJo V.7 (Treestar Inc., Ashland, OR, USA).

Goretzki A., Lin Y.J., Zimmermann J., Rainer H., Junker A.C., Wolfheimer S., Vieths S., Scheurer S, & Schülke S. (2022). Role of Glycolysis and Fatty Acid Synthesis in the Activation and T Cell-Modulating Potential of Dendritic Cells Stimulated with a TLR5-Ligand Allergen Fusion Protein. International Journal of Molecular Sciences, 23(20), 12695.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!