A clone of Caco-2 cells, TC7, was used in this study because differentiated TC7 cells form a regular epithelial monolayer (Chantret et al., 1994 (link)). Caco-2/TC7 cells were kindly provided by Dr. A. Zweibaum (INSERM, U170, Villejuif, France) and were identified as Caco-2 cells by STR profiling (DSMZ ACC 169). Cells were tested negative for mycoplasma with Mycostrip kits (Invivogen). Prior experiments, cells were seeded at a low concentration of 105 cells on a 24 mm polyester filter with 0.4 µm pores (3450, Corning Inc, Corning, NY). Cells were maintained in Dulbecco’s modified Eagle’s minimum essential medium supplemented with 20% heat-inactivated fetal bovine serum and 1% non-essential amino acids (Gibco, Waltham, MA), and cultured in 10% CO2/90% air. The medium was changed every 48 hr.
Dulbecco s modified eagle s minimum essential medium
Manufactured by Thermo Fisher Scientific
Dulbecco's modified Eagle's minimum essential medium (DMEM) is a widely used cell culture medium formulation designed to support the growth and maintenance of various cell types. It provides a balanced salt solution and essential nutrients required for cell survival and proliferation.
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3 protocols using dulbecco s modified eagle s minimum essential medium
1
Caco-2/TC7 Cell Culture Protocol
2
Caco-2 Cell Differentiation Protocol
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A clone of Caco-2 cells, TC7, was used in this study because differentiated TC7 cells form a regular epithelial monolayer (Chantret et al., 1994) . Cells were seeded at a low concentration of 10 5 cells on a 24 mm polyester filter with 0.4 µm pores (3450, Corning inc., Corning, NY). Cells were maintained in Dulbecco's modified Eagle's minimum essential medium supplemented with 20% heat-inactivated fetal bovine serum and 1% non-essential amino acids (Gibco, Waltham, MA), and cultured in 10% CO2/90% air.
The medium was changed every 48 hours.
The medium was changed every 48 hours.
3
Culturing C6 and MDA-MB-231 Cancer Cells
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