Il2 percpefluor710

Manufactured by Thermo Fisher Scientific

The IL2-PerCPeFluor710 is a fluorescently labeled antibody that binds to the Interleukin-2 (IL-2) cytokine. It is designed for use in flow cytometry applications to detect and quantify IL-2 expression in biological samples.

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Lab products found in correlation

Cd4 alexa fluor 700, by Thermo Fisher Scientific (2 mentions) Ifn γ efluor450, by Thermo Fisher Scientific (2 mentions) Foxp3 alexa fluor 488, by BioLegend (2 mentions) Cd127 pe, by Miltenyi Biotec (2 mentions) Foxp3 buffer set, by Thermo Fisher Scientific (2 mentions) Streptavidin alexa fluor 700, by Thermo Fisher Scientific (2 mentions) Brilliant violet 421 cd25, by BioLegend (2 mentions) Streptavidin ecd, by Beckman Coulter (2 mentions) Live dead fixable aqua staining kit, by Thermo Fisher Scientific (2 mentions) Cd45ro ecd, by Beckman Coulter (2 mentions) Cd3 apc vio770, by Miltenyi Biotec (1 mentions) Cd4 apc vio770, by Miltenyi Biotec (1 mentions) Tnf α pe cy7, by BD (1 mentions) Cd25 apc, by BD (1 mentions) Cd8 bv421, by BioLegend (1 mentions) Cd14 bv510, by BioLegend (1 mentions) Cd56 pe cy7, by BioLegend (1 mentions) Cd3 bv605, by BioLegend (1 mentions) Tnf α fitc, by BioLegend (1 mentions) Facsdiva 8, by BD (1 mentions)

3 protocols using il2 percpefluor710

Comprehensive Immune Phenotyping Protocol

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All staining experiments were performed at 4°C for 30 minutes. Antibodies used were CD3-PerCPCy5.5, CD3-AF700, CD4-Brilliant Violet 605, CD8-APC-Cy7, CD25-APC, CD134-PE, OX40-APC, TNF-α-PECy7, CD154-APC, CD152-PCF594, Tbet-PerCPCy5.5, PD1-PeCy7, CD15s-Brilliant Violet 421, Helios-PE, ICOS-PeCy7, CD39-Brilliant Violet 711 (Becton Dickinson (BD) Biosciences), CD4-Alexa Fluor 700, IFN-γ-eFluor450, IL2-PerCPeFluor710, Streptavidin-Alexa Fluor 700 (eBioscience), FoxP3-Alexa Fluor 488, CD25-Brilliant Violet 421 (BioLegend), CD39-biotin, CD127-PE, CD4-APCVio770, CD3-APCVio770 (Miltenyi biotec), Streptavidin-ECD, CD45RO-ECD (Beckman Coulter). LIVE/DEAD fixable aqua staining kit (Life technologies) was used to discriminate live and dead cells. For intracellular staining, FoxP3 buffer set (eBioscience) was used. Cell acquisition was performed by an LSR II (Becton Dickinson) and analyses were performed using FlowJo software.

Brezar V., Hani L., Surenaud M., Hubert A., Lacabaratz C., Lelièvre J.D., Levy Y, & Seddiki N. (2017). Negative modulation of suppressive HIV-specific regulatory T cells by IL-2 adjuvanted therapeutic vaccine. PLoS Pathogens, 13(7), e1006489.

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Multiparametric Flow Cytometry Assay

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All staining experiments were performed at 4°C for 30 minutes. Antibodies used were CD3-PerCPCy5.5, CD8-APCCy7, CD25-APC, CD134-PE, TNF-α-PECy7, CD154-APC ((Becton Dickinson (BD) Biosciences)), CD4-Alexa Fluor 700, IFN-γ-eFluor450, IL2-PerCPeFluor710, Streptavidin-Alexa Fluor 700 (eBioscience), FoxP3-Alexa Fluor 488, CD25-Brilliant Violet 421 (BioLegend), CD39-biotin, CD127-PE (Miltenyi biotec), Streptavidin-ECD, CD45RO-ECD (Beckman Coulter). LIVE/DEAD fixable aqua staining kit (Life technologies) was used to discriminate live and dead cells. For intracellular staining, FoxP3 buffer set (eBioscience) was used.

Brezar V., Ruffin N., Richert L., Surenaud M., Lacabaratz C., Palucka K., Thiébaut R., Banchereau J., Levy Y, & Seddiki N. (2015). Decreased HIV-Specific T-Regulatory Responses Are Associated with Effective DC-Vaccine Induced Immunity. PLoS Pathogens, 11(3), e1004752.

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Multiparametric Flow Cytometry of Immune Cells

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The following fluorochrome-conjugated antibodies were used in this study: CD14 BV510, CD19 BV510, CD3 APC Fire 750 or CD3 BV605, CD4 PE/Dazzle 594, or CD4 APC Fire 750, CD8 BV421, CCR5 PE/Dazzle 594, CD56 PeCy7 (Biolegend) for surface antigens; and IFN-γ PeCy7, TNF-α FITC (Biolegend) and IL-2 PercP eFluor710 (eBioscience), for intracellular staining. PBMC were washed in PBS, and surface stained at 4°C for 20 min with saturating concentrations of different combinations of antibodies in the presence of fixable live/dead stain (Invitrogen). Cells were then fixed and permeabilized for detection of intracellular antigens. Cells were acquired on a BD Fortessa X20 using BD FACSDiva8.0 (BD Bioscience) and analysed using FlowJo 10 (Tree Star). Stochastic neighbor embedding (SNE) analysis was performed using the mrc.cytobank platform.

Gupta R.K., Abdul-jawad S., McCoy L.E., Mok H.P., Peppa D., Salgado M., Martinez-Picado J., Nijhuis M., Wensing A.M., Lee H., Grant P., Nastouli E., Lambert J., Pace M., Salasc F., Monit C., Innes A., Muir L., Waters L., Frater J., Lever A.M., Edwards S., Gabriel I.H, & Olavarria E. (2019). HIV-1 remission following CCR5Δ32/Δ32 haematopoietic stem cell transplantation. Nature, 568(7751), 244-248.

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