Nomo 1

We obtained human AML cell lines OCI-AML3, Sig-M5, NOMO-1, and OCI-M1 from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Brunswick, Germany) We obtained other human AML cell lines (HL-60, MV-4–11, THP-1, KG-1a, HEL and Kasumi-1) from American Tissue Culture Collection (ATCC, Manassas, Virginia). All cell lines were obtained during 2014–2015. Cell lines were incubated at 37°C under 5% CO_2, in RPMI 1640 plus 10% FBS and additional growth factors as necessary according to the tissue bank guidelines for each cell line. Mycoplasma testing was performed at least annually using the MycoAlert^™ (Lonza) detection kit. Cell lines identity was confirmed at the time of study using the ATCC Human STR Profiling Cell Authentication Service. Cell passage number was maintained below 20 culture splits.

A375, HEK 293T, THP-1, RS4;11, HT-1080, Jurkat, U937, and MCF-7 cells were purchased from ATCC. SET-2, HL-60, KG1, MOLM-13, MV4;11, NOMO1, NB4, OCI-AML2, OCI-AML3, HEL, KASUMI-1, TF-1, and K562 cells were purchased from DSMZ. DAOY, IMR5, ONS-76, RD-ES, and TC71 cells were gifts from Johannes Schulte and were verified using STR genotyping (Genetica DNA Laboratories). HEK 293T, DAOY, HT-1080, IMR, Jurkat, MCF-7, ONS-76, RDES, and TC71 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS). THP-1, RS4;11, HL-60, KG1 MOLM-13, MV4;11, NOMO1, NB4, OCI-AML2, HEL, KASUMI-1, K562, U937 SET2, and Jurkat cells were grown in RPMI-1640 medium with 10% FBS. CD45⁺ human cord blood (CB) cells (StemCell Technologies, Catalog 70008.3) were thawed in IMDM with 15% FBS and DNase1 (1 mg/mL) and grown in IMDM supplemented with 10% FBS, 1 × penicillin/streptomycin, 100 ng/mL TPO, 100 ng/mL SCF, and 100 ng/mL G-CSF. All cells were grown at 37 °C in a 5% CO₂ incubator. Cell viability assays were performed on CB cells immediately after thawing. Cell lines were tested for mycoplasma using the MycoAlert^TM Mycoplasma Detection Kit (Lonza).