Jsm 5400 scanning electron microscope

Manufactured by JEOL

Sourced in Japan, Germany

The JSM-5400 is a scanning electron microscope (SEM) manufactured by JEOL. It is designed to produce high-resolution images of sample surfaces by scanning them with a focused beam of electrons. The JSM-5400 can magnify samples up to 300,000 times and provides detailed information about the topography, composition, and other characteristics of the specimen.

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Lab products found in correlation

Emitech k850, by Quorum Technologies (1 mentions)

2 protocols using jsm 5400 scanning electron microscope

Morphological Characterization of Lyophilized Films

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For morphological characterization, samples were lyophilized as indicated in Section 2.5.1. Cross-sections of the lyophilized film samples were then cut using a cold scalpel. Finally, the samples were examined with a Jeol JSM-5400 scanning electron microscope (JEOL, Tokyo, Japan).

Antezana P.E., Municoy S., Orive G, & Desimone M.F. (2022). Design of a New 3D Gelatin—Alginate Scaffold Loaded with Cannabis sativa Oil. Polymers, 14(21), 4506.

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Scanning Electron Microscopy Protocol

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Fixation of treated cells was performed using 2.5 % glutaraldehyde. Thereafter, cells were dehydrated in a graded ethanol series (70, 90, 100, 100, 100 %, and acetone), desiccated by critical point drying (Emitech K850; Quorum Technologies LTD, West Sussex, UK), mounted, sputtered with gold–palladium (Polaron CA 508; Fisons Instruments, Mainz-Kastel, Germany) and examined with a JEOL JSM—5400 scanning electron microscope (Eching, Germany).

Messner B., Türkcan A., Ploner C., Laufer G, & Bernhard D. (2015). Cadmium overkill: autophagy, apoptosis and necrosis signalling in endothelial cells exposed to cadmium. Cellular and Molecular Life Sciences, 73, 1699-1713.

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