OE33, LIM1215 and the KM12 cell lines were maintained in RPMI media (Gibco, UK). COLO320, HCT116 and HT29 cell lines were maintained in Macoy’s5A media (Gibco, UK). Cell culture media was supplemented with 10% foetal bovine serum (Gibco, UK), 5% Penicillin/Streptomycin (Gibco, UK) and 2mM L-glutamine (Gibco, UK). All cells were maintained at 37 °C in a 5% CO2 humidified atmosphere and were regularly screened for the presence of mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza, Switzerland).
Macoy s5a media
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Macoy's5A media is a laboratory culture medium used for the growth and cultivation of microorganisms. It provides a balanced nutritional composition to support the general growth of a wide range of bacterial species.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi media, by Thermo Fisher Scientific (1 mentions)
Mycoalert mycoplasma detection kit, by Lonza (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Multi well plate, by Corning (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Gelatin, by Merck Group (1 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Matrigel, by Merck Group (1 mentions)
N acetylcysteine, by Merck Group (1 mentions)
Diethylene glycol, by Merck Group (1 mentions)
Streptomycin, by Welgene (1 mentions)
2 protocols using macoy s5a media
1
Cell Line Maintenance Protocol
2
Reagents and Materials for Cell-based Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RPMI and Macoy's 5A media were purchased from Gibco. Fetal bovine serum (FBS) was purchased from Thermo Scientific. Streptomycin and penicillin were purchased from Welgene Inc. Sodium carbonate, Folin–Ciocalteu's reagent, gallic acid, diethylene glycol, sodium hydroxide, quercetin, (+)‐catechin, dimethyl sulfoxide (DMSO), N‐acetylcysteine (NAC), 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyl tetrazolium bromide (MTT), RNase, propidium iodide (PI), 2′,7′‐dichlorofluorescein diacetate (DCFH‐DA), 3,3′‐dihexyloxacarbocyanine iodide (DiOC6), Matrigel, crystal violet, sodium dodecyl sulfate, and gelatin were purchased from Sigma‐Aldrich (St. Louis). Multi‐well plates were purchased from Corning Inc. All other chemicals and solvents (LC grade) were purchased from Fisher Scientifics.
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