The mineralogy of the dolostone samples is determined by a Rigaku RAPID II or a Bruker D8 Advance x-ray diffractometer. The Rigaku RAPID II instrument operates at 50 kV and 90 mA on a rotating Mo anode x-ray source; the Bruker D8 Advance instrument runs at 40 kV and 40 mA with a Cu anode x-ray source. X-ray diffraction data processing and mineral identification are performed using Jade 6.5 software. The relative abundance of dolomite (104), calcite (104), and quartz (101) is estimated from the area of characteristic diffraction peaks (53 ). Textural analysis of the halite samples is performed on a Hitachi SU1510 Variable Pressure SEM, and the chemical compositions are characterized using EDS equipped with SEM.
Su1510 variable pressure sem
Manufactured by Hitachi
Sourced in Japan
The Hitachi SU1510 Variable Pressure SEM is a scanning electron microscope designed for high-resolution imaging and analysis. It features variable pressure capabilities, allowing for the examination of samples in a controlled gaseous environment. The SU1510 enables non-destructive imaging and analysis of a wide range of materials, including those that are non-conductive or sensitive to high-vacuum conditions.
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3 protocols using su1510 variable pressure sem
1
Quantitative Mineralogy of Dolostone
2
Enamel Surface Topography Analysis
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The surface topography of the enamel specimens at 1200× magnification was studied under a scanning electron microscope (Hitachi SU-1510 Variable Pressure SEM, Hitachi Ltd., Tokyo, Japan) at 15 kV in high-vacuum mode. The SEM micrographs of all enamel blocks were taken on two occasions: (1) before the immersion procedures, where the enamel blocks were mounted onto an aluminium stub separately to assess their surface topography without coating, and (2) after 20 rounds of immersion, where all the specimens were sputter-coated with carbon at 15 kV and the entire buccal surface was scanned. The SEM micrographs provided visual and illustrative comparisons of the surface morphologic changes of specimens before and after the drug/water challenge.
3
Isolation and Fixation of Microparticles
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A sample run was performed according to the processing flow described in the sample processing subsection using 100 μl of cell-free plasma. After the final rinse of the cluster, a 2.5% glutaraldehyde solution was withdrawn in the capillary to fix the MPMs. After a 5 min incubation, the fixing solution was removed by washing with PBS for 10 minutes. Microparticles were dehydrated using an ethanol dilution series at 10 minutes each at 30%, 50%, 75%, 95% and 99.7%. Finally, the cluster was released onto a conductive carbon adhesive film and analyzed at 100 mbar using a Hitachi SU1510 Variable Pressure SEM.
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