Cells were seeded in 24-well plates at densities of either 8 × 104 cells/well or 2–3 × 104 cells/well if they were transfected with siRNA prior to TKI treatment. The growth medium was replaced by serum-free medium for 16 h. Afterwards, the cells were treated with 10 µM, 1 µM, and 0.1 µM AZD9291 (ApexBio Technology, Houston, TX, USA) or erlotinib (Cell Signaling Technology, Frankfurt A.M., Germany) in growth medium for 24 h. As a control, growth medium with 0.1% DMSO was used. The experiments were repeated three times with three or four technical repeats each.
Azd9291
Manufactured by Apexbio
Sourced in United States
AZD9291 is a laboratory research tool used for the study of cellular and molecular processes. It is a selective inhibitor that targets specific enzymes or proteins. The core function of AZD9291 is to enable researchers to investigate the effects of its inhibition on various biological systems.
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Lab products found in correlation
2 protocols using azd9291
1
Evaluation of TKI Cytotoxicity in Cancer Cells
2
Morusin Inhibits LPS-Induced EGFR Signaling
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When the REC monolayers reached 70–80% confluency, the cells were pretreated with EGFR inhibitor, the AZD-9291 (APExBIO Technology LLC, Shanghai, China), at a concentration of 500 nM or vehicle (1% DMSO) for 1 h, followed by treating without or with 0.1 µg/mL LPS for 3 h after pretreating with 50 µg/mL of Morusin for 12 h or not (n = 4, respectively). Five treatments were contained, and after the treatment, immunoblots targeting the protein expression of p-EGFR/EGFR, p-AKT/AKT, p-IκBα/IκBα, IκBα/β-actin, and p-p65/p65 were performed as mentioned above.
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