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2 protocols using benzene 1 3 5 triol

1

Integrated Gut Microbiome Modulation

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Formic acid, bile salts, soluble starch, (+)-arabinogalactan, tryptone, yeast extract, xylan from birchwood, L-cysteine hydrochloride monohydrate, guar gum, inulin, Tween 80, buffered peptone water, Dulbecco′s phosphate buffer saline (PBS), casein sodium salt from bovine milk, pectin from citrus fruits, mucin from porcine stomach-type III, CaCl2, KCl, NaCl, NaHCO3, anhydrous K2HPO4, KH2PO4, MgSO4 monohydrate, FeSO4 heptahydrate, resazurin redox indicator, quercetin, kaempferol, quercetin-3-O-rutinoside (aka rutin), phenylacetic acid, 4′-hydroxyphenylacetic acid, 3′-hydroxyphenylacetic acid, 3′,4′-dihydroxyphenylacetic acid, 3-phenylpropanoic acid, 3-(4′-hydroxyphenyl)propanoic acid, 3-(3′-hydroxyphenyl)propanoic acid, 3-(3′,4′-dihydroxyphenyl)propanoic acid (aka dihydrocaffeic acid), 4-hydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid (aka protocatechuic acid), and benzene-1,3,5-triol (aka phloroglucinol) were obtained from Sigma-Aldrich (St Louis, MO, USA). Isorhamnetin was from PhytoLab GmbH (Vestenbergsgreuth, Germany). All solvents and water were UHPLC-grade and were purchased from VWR International (Milan, Italy).
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2

Magnetic Nanoparticle Synthesis and Characterization

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FeCl2·4H2O, FeCl3·6H2O, benzene-1,4-diol (hydroquinone), benzene-1,3,5-triol (phloroglucinol), Folin–Ciocalteu (FC) reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, precursor chitosan (highly viscous) from crab shells, heparin sodium salt (Hep) from porcine intestinal mucosa, hydrochloric acid, ammonium persulfate, potassium thiocyanate, hydrogen peroxide, and cell counting kit-8 (CCK-8) were purchased from Sigma-Aldrich (Milwaukee, WI, USA). Ammonium hydroxide (30%) and glacial acetic acid were obtained from Lach-Ner (Neratovice, Czech Republic). Sodium carbonate was purchased from Lachema (Neratovice, Czech Republic). 3,4,5-Trihydroxybenzoic acid (gallic acid) was purchased from Loba Feinchemie (Fischamend, Austria). Dulbecco’s modified Eagle’s medium, minimum essential medium Eagle, sodium pyruvate, antibiotic-antimycotic solution (penicillin/streptomycin/amphotericin B), and 6-carboxy-2′,7′-dichlorohydrofluorescein diacetate (DCFH-DA) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fetal bovine serum and donor equine serum were purchased from HyClone Laboratories (Logan, UT, USA). All chemicals were used as received. Ultrapure water (Q-water), obtained using a Milli-Q Gradient A10 system (Millipore; Molsheim, France), was used during the synthesis and modification of the magnetic nanoparticles.
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