Hrp conjugated rat anti mouse igg kappa light chain

Manufactured by BD

Sourced in United States

The HRP)-conjugated rat anti-mouse IgG kappa light chain is a laboratory reagent used in immunological assays. It is an antibody-enzyme conjugate, where the anti-mouse IgG kappa light chain antibody is coupled to the enzyme horseradish peroxidase (HRP). This conjugate can be used to detect the presence and quantity of mouse immunoglobulin kappa light chain in samples.

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Lab products found in correlation

Enhanced chemiluminescence detection system, by Cytiva (2 mentions) Anti bcl11b, by Cell Signaling Technology (2 mentions) Hrp conjugated goat anti rabbit igg sc 2054, by Santa Cruz Biotechnology (2 mentions)

2 protocols using hrp conjugated rat anti mouse igg kappa light chain

Western Blot Analysis of BCL11B, Tax, and HBZ

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The western blot analyses were carried out as described previously.24 (link) The primary antibodies used at the indicated dilutions were anti-BCL11B (1/1000),16 (link) anti-BCL11B (1/1000) (Cell Signaling Technology, Beverly, MA, USA), anti-Tax (1/2000) (Taxy7),25 (link) anti-Tax2,26 (link) anti-α-tubulin (DM1A) (1/1000) (Calbiochem, San Diego, CA, USA) and α-HBZ (1/1000).9 (link) Horseradish peroxidase (HRP)-conjugated rat anti-mouse IgG kappa light chain (BD Biosciences, San Jose, CA, USA) and HRP-conjugated goat anti-rabbit IgG (sc-2054; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used as secondary antibodies (1/10 000). Immunoreactive bands were visualized with the Enhanced Chemiluminescence Detection System (Amersham Pharmacia Biotech, Orsay, France).

Takachi T., Takahashi M., Takahashi-Yoshita M., Higuchi M., Obata M., Mishima Y., Okuda S., Tanaka Y., Matsuoka M., Saitoh A., Green P.L, & Fujii M. (2015). Human T-cell leukemia virus type 1 Tax oncoprotein represses the expression of the BCL11B tumor suppressor in T-cells. Cancer Science, 106(4), 461-465.

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Western Blot Analysis of HTLV-1 Proteins

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The western blot analyses were carried out as described previously.^{(24 (link))} The primary antibodies used at the indicated dilutions were anti-BCL11B (1/1000),^{(16 (link))} anti-BCL11B (1/1000) (Cell Signaling Technology, Beverly, MA, USA), anti-Tax (1/2000) (Taxy7),^{(25 (link))} anti-Tax2,^{(26 (link))} anti α-tubulin (DM1A) (1/1000) (Calbiochem, San Diego, CA, USA) and α-HBZ (1/1000).^{(9 (link))} Horseradish peroxidase (HRP)-conjugated rat anti-mouse IgG kappa light chain (BD Biosciences, San Jose, CA, USA) and HRP-conjugated goat anti-rabbit IgG (sc-2054; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used as secondary antibodies (1/10 000). Immunoreactive bands were visualized with the Enhanced Chemiluminescence Detection System (Amersham Pharmacia Biotech, Orsay, France).

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