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Cleaved caspase 3 elisa

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The Cleaved caspase 3 ELISA is a quantitative enzyme-linked immunosorbent assay designed to measure the levels of cleaved caspase 3 in biological samples. Caspase 3 is a key executioner caspase that plays a central role in the apoptosis pathway. The ELISA kit utilizes a sandwich format, where a specific antibody is used to capture the cleaved caspase 3 protein, and a detection antibody is used to quantify the levels of the captured protein.

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Lab products found in correlation

Tnfα elisa, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Cleaved caspase-3 (3596 citations) Caspase-3 (2138 citations) Caspases (2 citations)

5 protocols using cleaved caspase 3 elisa

1

Caspase 3 ELISA Quantification

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A cleaved caspase 3 ELISA was done according to manufacturer’s instructions (Cell Signaling Technology, Danvers, MA) with equal protein loaded to allow for comparison using the optical density measurement (O.D).
Liu L., Jiang Y., Curtiss E., Fukuchi K.I, & Steinle J.J. (2017). TLR4 regulates insulin resistant proteins to increase apoptosis in the mouse retina. Inflammation research : official journal of the European Histamine Research Society ... [et al.], 66(11), 993-997.
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2

Caspase 3 and TNFα ELISA Protocols

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A cleaved caspase 3 ELISA (Cell Signaling Corp, Danvers, MA) was done according to the manufacturer's instructions on both mouse whole retinal lysates and REC. A TNFα ELISA (Fisher Scientific, Pittsburgh, PA) was performed based upon the manufacturer's instructions with the following exceptions: 100 μg protein of each sample was added into wells, and the incubation of samples and primary antibody occurred for 24 h at 4°C.
Curtiss E., Jiang Y., Liu L., Hawthorne C., Zhang J, & Steinle J.J. (2018). Epac1 Restores Normal Insulin Signaling through a Reduction in Inflammatory Cytokines. Mediators of Inflammation, 2018, 3809092.
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3

Quantification of Cleaved Caspase-3 and Insulin

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A cleaved caspase 3 ELISA was done according to manufacturer’s instructions (Cell Signaling Corp, Danvers, MA). An insulin ELISA was done according to manufacturer’s instructions (Sigma, St. Louis, MO).
Jiang Y., Liu L, & Steinle J.J. (2018). miRNA15a regulates insulin signal transduction in the retinal vasculature. Cellular signalling, 44, 28-32.
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4

Measuring Apoptosis Markers in Cell Lysates

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A TNFα ELISA (Fisher Scientific, Pittsburgh, PA) was used according to manufacturer’s instructions on cell lysates (collected into RIPA lysis buffer) with the exception that sample exposure to primary antibody occurred for 24hrs. One hundred micrograms of protein were used to insure equal protein amounts in all wells. A cleaved caspase 3 ELISA (Cell Signaling Technology, Danvers, MA) was done according to manufacturer’s instructions with equal protein concentrations loaded in all wells so analyses could be done using the optical density (O.D.)
Liu L., Jiang Y, & Steinle J.J. (2017). Inhibition of HMGB1 protects the retina from ischemia-reperfusion, as well as reduces insulin resistance proteins. PLoS ONE, 12(5), e0178236.
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5

Cleaved Caspase-3 ELISA Assay

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A cleaved caspase 3 ELISA (Cell Signaling, Danvers, MA) was done according to manufacturer’s instructions. For the cleaved caspase 3 ELISA, equal protein concentrations were loaded into each well to allow for analyses based on the optical density measurement.
Liu L, & Steinle J.J. (2018). Toll-Like Receptor 4 Regulates Insulin Signal Transduction in Retinal Müller Cells. Growth factors (Chur, Switzerland), 35(6), 234-238.
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