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Fluorescein isothiocyanate fitc conjugated bsa

Manufactured by Merck Group
Sourced in Sao Tome and Principe, United States

Fluorescein isothiocyanate (FITC) conjugated bovine serum albumin (BSA) is a fluorescently-labeled protein compound. FITC is a fluorescent dye that is covalently attached to the BSA molecule, allowing it to be used as a tracer or labeling agent in various laboratory applications.

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2 protocols using fluorescein isothiocyanate fitc conjugated bsa

1

Immunofluorescence Assay for Cell Adhesion

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All cell culture reagents were purchased from Life Technologies (Carlsbad, CA). Anti-Human IgG (Fc specific), Bovine Serum Albumin (BSA), and fluorescein isothiocyanate (FITC) conjugated BSA were from Sigma-Aldrich (St Louis, MO). P-selectin-IgG Fc (P-selectin) and ICAM-IgG Fc (ICAM) were purchased from R&D Systems (Minneapolis, MN). 35mm non-tissue culture treated polystyrene dishes were purchased from Corning (Corning, NY). Polydimethylsiloxane (PDMS) base and curing agent were from Ellsworth Adhesives (Germantown, WI, USA). Cell lines were obtained from the American Type Culture Collection (Manassas, VA).
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2

Optimizing Human tPA Activity

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Human t-PA (rt-PA; Actilyse®) was purchased from Boehringer Ingelheim GmbH (Rhein, Germany) and dialysed against 0.4M HEPES pH 7.4 to remove the original vehicle components [5 (link)]. Human Glu-plasminogen was from Enzyme research laboratories (South Bend, IN, USA). Fluorescein isothiocyanate (FITC)-conjugated BSA, bovine aprotinin and endothelial cells growth supplement (ECGS) were obtained from Sigma Aldrich (St Louis, MO, USA). HA1077 (fasudil; hydrochloride) was purchased from Cayman Chemicals (Ann Arbor, MI, USA) while KD025 (SLx-2119) from MedChem Express (Princeton, NJ, USA). Matrigel was obtained from BD Biosciences (Australia) and rat collagen type-I from Trevigen (Gaithersburg, MD, USA). All TaqMan® gene expression assays and reagents were purchased from Applied Biosystems (Thermo Fisher Scientific, Australia).
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