Mice were restrained in a 50 mL falcon tube. Each tube had 2 holes drilled into the bottom, as well as in the lid to allow the animals to breathe normally and move their tail. After 4 h, animals were removed from the tube, deeply anesthetized with isoflurane and sacrificed by decapitation. Control animals were kept undisturbed in their home cage until sacrifice. Trunk blood was collected in labeled 1.5 mL EDTA-coated microcentrifuge tubes (Sarstedt, Germany) and kept on ice until centrifugation. After centrifugation (4°C, 8000 rpm for 15 min) plasma was removed and transferred to new, labeled tubes and stored at −20°C until corticosterone quantification. For mRNA analysis, brains were removed, snap-frozen in isopentane at −40°C, and stored at −80°C for in situ hybridization.
1.5 ml edta coated microcentrifuge tubes
Manufactured by Sarstedt
Sourced in Germany
The 1.5 mL EDTA-coated microcentrifuge tubes are designed for the collection, storage, and transportation of biological samples. The EDTA (Ethylenediaminetetraacetic acid) coating helps prevent blood clotting, making them suitable for blood sample handling.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 1.5 ml edta coated microcentrifuge tubes
1
Acute Stress Response in Mice
2
Acute Stress Response in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were restrained in a 50 mL falcon tube. Each tube had 2 holes drilled into the bottom, as well as in the lid to allow the animals to breathe normally and move their tail. After 4 h, animals were removed from the tube, deeply anesthetized with isoflurane and sacrificed by decapitation. Control animals were kept undisturbed in their home cage until sacrifice. Trunk blood was collected in labeled 1.5 mL EDTA-coated microcentrifuge tubes (Sarstedt, Germany) and kept on ice until centrifugation. After centrifugation (4°C, 8000 rpm for 15 min) plasma was removed and transferred to new, labeled tubes and stored at −20°C until corticosterone quantification. For mRNA analysis, brains were removed, snap-frozen in isopentane at −40°C, and stored at −80°C for in situ hybridization.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!