Jem 1200ex tem

Multiple ultrathin sections (of 70–80 nm) were cut on a Reichart Ultracut E, collected on Formvar coated copper slot grids, post-stained with uranyl acetate (saturated solution) and Reynold’s lead citrate, and initially imaged at a JEOL JEM-1200EX TEM with a 1k CCD camera (GATAN) in order to get overview maps of the sections and localize the cells of interest. Then, for high-resolution images of these cells, we imaged them at a 200 kV Tecnai F20 intermediate voltage TEM (FEI, Inc., Hillsboro, OR, USA) with a 4k CCD camera (GATAN), at 19,000× magnification (1.12 nm pixel size). For large overviews of the cells at medium magnification, we acquired montages of overlapping images in an automated fashion using the microscope control software SerialEM.^{60 (link)}