Native ldl nldl

Manufactured by Merck Group

Sourced in United States

Native LDL (nLDL) is a laboratory-prepared low-density lipoprotein (LDL) product. It is a component of the lipoprotein system and plays a role in cholesterol transport within the body.

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Lab products found in correlation

Annexin 5 fitc kit, by Thermo Fisher Scientific (1 mentions) Huvec cell line, by American Type Culture Collection (1 mentions) Ldh assay kit, by Nanjing Jiancheng (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Dulbecco s modified eagle s media dmem, by Merck Group (1 mentions) Allicin, by Merck Group (1 mentions) Dmem f12, by Merck Group (1 mentions) N acetylcysteine nac, by Merck Group (1 mentions) Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions)

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Native LDL (3 citations)

3 protocols using native ldl nldl

Cytotoxicity and Apoptosis of Allicin in HUVEC

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HUVEC cell line was originally acquired from the American Type Culture Collection (Manassas VA, USA). Native LDL (nLDL) was purchased from Sigma-Aldrich (St. Louis, Mo., USA). Dulbecco’s modified Eagle’s media (DMEM), dimethylsulfoxide (DMSO), and allicin were obtained from Sigma (St. Louis, MO, USA). LDH assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The AnnexinV-FITC kit was purchased from Invitrogen (Carlsbad, CA, USA). All other reagents were of analytical grade and purchased from Sigma-Aldrich (St. Louis, MO, USA).

Chen X., Pang S., Lin J., Xia J, & Wang Y. (2016). Allicin prevents oxidized low-density lipoprotein-induced endothelial cell injury by inhibiting apoptosis and oxidative stress pathway. BMC Complementary and Alternative Medicine, 16, 133.

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Oxidation of Native LDL Protocol

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Native LDL (nLDL) was purchased from Sigma. OxLDL was prepared by incubating nLDL with 10 μmol/L CuSO4 at 37°C for 24 hours. The material was dialyzed against a sterile solution (150 mmol/L NaCl, 1 mmol/L EDTA, 100 μg/mL polymyxin B, pH 7.4) and then sterilized by filtration. The extent of LDL oxidation was estimated by agarose gel electrophoresis and by measuring the amount of thiobarbituric acid reactive substances generated (Supplementary Fig S1A and S1B).

Tie G., Yan J., Khair L., Messina J.A., Deng A., Kang J., Fazzio T, & Messina L.M. (2017). Hypercholesterolemia Increases Colorectal Cancer Incidence by Reducing Production of NKT and γδ T Cells from Hematopoietic Stem Cells. Cancer research, 77(9), 2351-2362.

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Modulation of ARPE-19 Cells by Oxidative Stress

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The human Retinal Pigment Epithelial cells, ARPE-19 (ATCC-CRL-2302) was cultured using Dulbecco’s Modified Eagle medium/Ham’s F12 medium (DMEM/F-12; Sigma-Aldrich, USA) supplemented with 10% Fetal Bovine Serum (FBS; Gibco, USA), Antibiotic-Antimycotic solution (Gibco, USA). At 80% confluency, they were used for the experiments in DMEM/F-12 supplemented with 1% FBS (low serum media). The cells were then exposed to 50 μg/mL oxLDL, 50 μg/mL Native LDL (N.LDL), 500 μM Hcy (Sigma-Aldrich, USA), 500 nM HCTL (Sigma-Aldrich, USA), 100 μg/mL AGE, 200 μM H₂O₂ (Merck, USA) and pre-treatment with 5 mM N-acetylcysteine (NAC; Sigma-Aldrich, USA).

AnandBabu K., Sen P, & Angayarkanni N. (2019). Oxidized LDL, homocysteine, homocysteine thiolactone and advanced glycation end products act as pro-oxidant metabolites inducing cytokine release, macrophage infiltration and pro-angiogenic effect in ARPE-19 cells. PLoS ONE, 14(5), e0216899.

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