Model 2996

Manufactured by Waters Corporation

Sourced in United States

The Waters Corporation Model 2996 is a photodiode array detector designed for use in liquid chromatography (LC) systems. It is capable of monitoring multiple wavelengths simultaneously and provides high-resolution detection of analytes eluting from the LC column.

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Close spelling variants of this product

Model 2996 PDA detector (3 citations) Model 2996 PDA absorbance detector (3 citations) Model 2996 photodiode array detector (2 citations) Waters model 2996 (2 citations)

5 protocols using model 2996

Monosaccharide Composition Analysis of Rice Straw Prehydrolyzates

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The monosaccharide
composition of the lyophilized rice straw prehydrolyzates was determined
using a Waters Alliance HPLC system (Model 2695, Waters Corporation,
Milford, MA) equipped with a SP-G precolumn, SP0810 analytical column
(Shodex, Kawasaki, Japan), and refractive index detector (Model 2414,
Waters Corporation, Milford, MA). The xylo-oligosaccharide concentration
was determined by equipping the HPLC described above with a Bio-Rad
Aminex-HPX 42A analytical column (Bio-Rad, Hercules, CA) and a Micro-Guard
deashing precolumn. Calibration curves for the xylo-oligosaccharides
(DP 2 to DP 6) were determined using pure (>95.0%) reference compounds.
The analytical columns and the detector were maintained at 85 and
50 °C, respectively. Millipore water was used as eluent at a
flow rate of 0.2 mL min^–1, and the sugars were quantified
using in-house calibration curves.
Analyses of formic acid,
acetic acid, HMF, and furfural were performed using the Waters Alliance
HPLC system fitted with a Bio-Rad Aminex HPX-87H ion exclusion analytical
column (Life Sciences Research, Hercules, CA) and photodiode array
detector (Model 2996, Waters Corporation, Milford, MA). The samples
were eluted with a 5 mM sulfuric acid at a flow rate of 0.6 mL min^–1 and were detected at 280 nm.²³

Rajan K, & Carrier D.J. (2014). Characterization of Rice Straw Prehydrolyzates and Their Effect on the Hydrolysis of Model Substrates Using a Commercial endo-Cellulase, β-Glucosidase and Cellulase Cocktail. ACS Sustainable Chemistry & Engineering, 2(9), 2124-2130.

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Synthesis and Characterization of Bioactive Compounds

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Platelet-activating factor (PAF; β-acetyl-γ-O-hexadecyl-L-α-phosphatidylcholine), formyl-met-leu-phe (fMLP), and Gue1654 (7-(methylthio)-2-[(2,2-diphenylacetyl)amino]benzo[1,2-d:4,3-d′]bisthiazole) were obtained from Sigma-Aldrich, whereas interkleukin-8 (IL-8) was purchased from R&D Systems. 5-Oxo-ETE³⁵ and LTB₄³⁶ were prepared by chemical synthesis as previously described. All reactions were carried out using dry solvents under argon atmosphere. High-resolution mass spectra were recorded on an AccuTOF mass spectrometer by positive ion ESI mode with DART as an ion source. ¹H NMR and ¹³C NMR spectra were recorded in CDCl₃ using TMS as an internal standard on a BRUKER AMX 400 MHz spectrometer at rt. All compounds were analyzed by TLC, HRMS and NMR. Prior to biological assay, the purity of all final compounds was determined to be >95% by NMR and HPLC. HPLC conditions: Waters 2695 Alliance System with Waters Novapak C18 (150 × 3.9 mm) column and photodiode array detector (Waters Model 2996), gradient mobile phase between H₂O/MeCN/MeOH (56:22:22) to H₂O/MeCN/MeOH (16:42:42) over 30 min, both solvents contained 0.02% acetic acid, and a flow rate was 1 mL/min.

Gore V., Gravel S., Cossette C., Patel P., Chourey S., Ye Q., Rokach J, & Powell W.S. (2014). Inhibition of 5-oxo-6,8,11,14-eicosatetraenoic acid-induced Activation of Neutrophils and Eosinophils by Novel Indole OXE Receptor Antagonists. Journal of medicinal chemistry, 57(2), 364-377.

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Electrochemical Simulation of C-1305 Metabolism

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The simulation of the oxidative metabolism of C-1305 was accomplished in an amperometric electrochemical thin-layer cell equipped with a disc glassy carbon (GC) working electrode (φ = 8 mm; A = 0.502 cm²) and a Pd/H₂ reference electrode (reactor cell; Antec Leyden, Zoeterwoude, the Netherlands). Carbon-loaded polytetrafluoroethylene (PTFE) served as auxiliary electrode. The cell potentials were applied using a ROXY EC System (Antec Leyden). All potentials mentioned in this work were based on the reference electrode. The software used for controlling EC was Dialogue (Antec Leyden).
The outlet of the electrochemical cell was interfaced into an ESI source of a quadrupole-time of flight (Q-TOF) mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) inlet for on-line EC/MS analysis using PEEK tubing. For controlling the MS, MassHunter software (Agilent Technology) was used. The electrochemically generated oxidation products were also off-line injected onto the LC column, separated, and detected by ESI-MS. LC separations were performed with Waters Associates HPLC system (Waters Co., Milford, MA, USA). It was equipped with a model 600 E system controller, a model 7725i Rheodyne injector, and a model 2996 photodiode array detector (DAD) controlled with Millennium software (Waters Co.).

Potęga A., Żelaszczyk D, & Mazerska Z. (2020). Electrochemical and in silico approaches for liver metabolic oxidation of antitumor-active triazoloacridinone C-1305. Journal of Pharmaceutical Analysis, 10(4), 376-384.

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HPLC Analysis of Mono and Disaccharides

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Mono and disaccharides were measured using an HPLC from Waters Corporation (Milford, MA) consisting of a pump (model 600, Waters Corporation) and an auto sampler model 717 Plus equipped with a refractive index detector (model 2414, Waters Corporation). Organics acids are monitored using a PDA detector (model 2996, Waters Corporation). The column used for the separation is Transgenomic ICSep IC-ION-300 (300 mm×7.8 mm OD) (Transgenomics, San Jose, CA, USA). The mobile phase is 0.01N H2SO4 at 0.4 mL min⁻¹. Analysis is carried out at 35°C.

Haddad M., Vali H., Paquette J, & Guiot S.R. (2014). The Role of Carboxydothermus hydrogenoformans in the Conversion of Calcium Phosphate from Amorphous to Crystalline State. PLoS ONE, 9(2), e89480.

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Quantification of C-2028 Cellular Uptake

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To receive concentration profiles of C-2028 in the selected cells treated with unbound C-2028 or its QD_green-β-CD(C-2028)-FA nanoconjugate, the obtained cell lysates were analyzed directly by reversed-phase HPLC. Analysis was performed using Waters Associates HPLC system (Waters Co., Milford, MA, USA) consisting of a model 600E system controller, a model 7725i Rheodyne injector, 717 plus Autosampler, and a model 2996 photodiode array detector controlled with Empower 3 software (Waters Co., USA). Chromatographic separation was achieved using a reversed-phase Suplex pKb-100 C18 analytical column (4.6 × 150 mm, 5-µm particle size) (Supelco, Inc., Bellefonte, PA, USA). The mobile phase consisted of a mixture of (A) water with 0.1% formic acid, and (B) methanol with 5% water. 50 mL of each sample was injected into the HPLC column at a flow rate of 0.6 mL·min⁻¹ with the following linear gradient elution: 15% B from 0–15 min, 100% B from 15–17 min, 100% B from 17–17.5, 15% B from 17.5–5 min (returning to initial conditions). The autosampler and the column were kept at 4 °C and RT, respectively. The eluate was monitored with UV-Vis detection at 420 nm and/or photodiode array and multiple wavelength detection.

Pilch J., Potęga A., Kowalczyk A., Kasprzak A., Kowalik P., Bujak P., Paluszkiewicz E., Augustin E, & Nowicka A.M. (2023). pH-Responsive Drug Delivery Nanoplatforms as Smart Carriers of Unsymmetrical Bisacridines for Targeted Cancer Therapy. Pharmaceutics, 15(1), 201.

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