Difenoconazole

B. cinerea strain B05.10 was obtained from the VTT culture collection (http://culturecollection.vtt.fi, ref. D-071295). To prepare conidial suspension, a mycelial disc of B. cinerea was inoculated onto malt extract agar (Oxoid, Basingstoke, UK) plates and incubated for 2 weeks at 22ºC. The conidia were harvested by flooding the cultures with sterile distilled water or potato dextrose broth (PDB; BD Biosciences, Spark, MD, USA), rubbing the surface with a spreader, and then filtered through four sheets of Miracloth (Chicopee Mills Inc., Milltown, NJ, USA). Schizophyllum commune KUC9080 isolated from a soil of Gurye, Korea was used to construct a microbial culture extract library. The KUC9080 strain was maintained on potato dextrose agar (PDA) plates. Tebuconazole and chlorothalonil were obtained from Kyung Nong Co. Ltd (Seoul, Korea), and boscalid, dichlofluanid, fenhexamid, fludioxonil, iprodione, difenoconazole, fenarimol, fenbuconazole, fenpropimorph, imazalil, and prochloraz in this study were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Chlorantraniliprole (98% purity, CAS 500008‐45‐7) and flubendiamide (98.1%, CAS 272451‐65‐7) were obtained in‐house at Bayer AG. Cyprodinil (99%, CAS 121552‐61‐2), iprodione (99.6%, CAS 1215631‐57‐4), boscalid (99.5%, CAS 188425‐85‐6), pyraclostrobin (99.9%, CAS 175013‐18‐0), chlorothalonil (99%, CAS 1897‐45‐6), difenoconazole (99.5%, CAS 119446‐68‐3), fenbuconazole (99%, CAS 114369‐43‐6), metconazole (98.9%, CAS 125116‐23‐6) and propiconazole (99%, CAS 60207‐90‐1) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). The fluorescent probe 7‐benzyloxymethoxy‐4‐(trifluoromethyl)‐coumarin (BOMFC; CAS 277309‐33‐8) was synthesized by Enamine Ltd. (Riga, Latvia) with a purity of 95%. HPLC gradient grade acetonitrile was purchased from Merck (Darmstadt, Germany). Unless otherwise mentioned all other reagents were of analytical grade and obtained from Sigma‐Aldrich (St. Louis, MO, USA).