The miR-18a mimic, inhibitor, and negative control miRNA were purchased from RiboBio (Guangzhou China) and transfected into cells at 100 nM concentrations via Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. KLF4 plasmid (1ug; Origene, Rockville, MD, USA) was also transfected into cells in the presence or absence of the miR-18a mimic by Lipofectamine 2000. After transfection twice in 48 h, cells were used in the subsequent experiments. RT-PCR and Western blotting were used to evaluate the transfection efficacy.
Klf4 plasmid
Manufactured by OriGene
Sourced in United States
The KLF4 plasmid is a laboratory tool containing the genetic sequence for the Kruppel-like factor 4 (KLF4) protein. KLF4 is a transcription factor involved in the regulation of gene expression. The plasmid can be used in various research applications, such as gene expression studies and cell line development.
Automatically generated - may contain errors
2 protocols using klf4 plasmid
1
Transfection Protocol for miR-18a Mimic and KLF4 Plasmid
2
Transfection of miR-92a and KLF4 in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The miR-92a mimic, inhibitor, and negative control miRNA were purchased from RiboBio (Guangzhou China) and transfected into cells at 100 nM concentrations via Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. KLF4 plasmid (1 µg; Origene, Rockville, MD, USA) was also transfected into cells in the presence or absence of the miR-92a mimic by Lipofectamine 2000. After transfection twice in 48 h, cells were used in the subsequent experiments. RT-PCR and Western blotting were used to evaluate the transfection efficacy.
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