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ITPR1 is a calcium release channel that regulates the release of calcium from the endoplasmic reticulum. It plays a crucial role in calcium signaling within cells.

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Lab products found in correlation

Normal serum, by Jackson ImmunoResearch (2 mentions) Ptrka, by Abcam (2 mentions) Pitpr1, by Cell Signaling Technology (2 mentions) Alexa fluor 594 conjugated second antibodies, by Jackson ImmunoResearch (2 mentions) Vinculin, by Abcam (2 mentions) Alexa fluor 488, by Jackson ImmunoResearch (2 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) Vdac1, by Abcam (1 mentions) Uqcrc2, by Abcam (1 mentions) P616 drp1, by Cell Signaling Technology (1 mentions) Cox 2, by Cell Signaling Technology (1 mentions) Cdkn1a p21, by Cell Signaling Technology (1 mentions) P mdm2, by Cell Signaling Technology (1 mentions) Ndufa9, by Abcam (1 mentions) Slc25a46, by Proteintech (1 mentions) Tomm20, by Santa Cruz Biotechnology (1 mentions) Lamp1, by Santa Cruz Biotechnology (1 mentions) Flag m2, by Merck Group (1 mentions)

3 protocols using itpr1

1

Tissue Immunostaining and Quantification

Cited 2 times
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Tissue immunostaining was performed, as previously described.84 (link) Tissue sections and cell cultures were fixed by 4% PFA in PBS for 30 min, permeabilized in 0.3% Triton X-100 for 1.5 h at room temperature, and blocked in 4% normal serum (Jackson ImmunoResearch Laboratories, West Grove, PA) for 20 min. Primary antibodies specific for CA8, V5, vinculin, TrkA, pTrkA (Abcam, Cambridge, MA), pITPR1 and ITPR1 (Cell Signaling Technology, Danvers, MA). Antibodies were allowed to incubate with sections and cell cultures overnight at 4°C after dilution in PBS containing 0.1% Triton X-100. Sections and cultures were washed three times for 10 min each in PBS, and incubated with Alexa Fluor 488 and/or Alexa Fluor 594-conjugated second antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA) for 1 h at RT. Sections and cultures were mixed with the 2nd antibody to assess the total number of cells, and images were acquired as described elsewhere.24 (link)
Zhuang G.Z., Upadhyay U., Tong X., Kang Y., Erasso D.M., Fu E.S., Sarantopoulos K.D., Martin E.R., Wiltshire T., Diatchenko L., Smith S.B., Maixner W, & Levitt R.C. (2018). Human Carbonic Anhydrase-8 AAV8 Gene Therapy Inhibits Nerve Growth Factor Signaling Producing Prolonged Analgesia and Anti-Hyperalgesia in Mice. Gene therapy, 25(4), 297-311.
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2

Tissue Immunostaining and Quantification

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue immunostaining was performed, as previously described.84 (link) Tissue sections and cell cultures were fixed by 4% PFA in PBS for 30 min, permeabilized in 0.3% Triton X-100 for 1.5 h at room temperature, and blocked in 4% normal serum (Jackson ImmunoResearch Laboratories, West Grove, PA) for 20 min. Primary antibodies specific for CA8, V5, vinculin, TrkA, pTrkA (Abcam, Cambridge, MA), pITPR1 and ITPR1 (Cell Signaling Technology, Danvers, MA). Antibodies were allowed to incubate with sections and cell cultures overnight at 4°C after dilution in PBS containing 0.1% Triton X-100. Sections and cultures were washed three times for 10 min each in PBS, and incubated with Alexa Fluor 488 and/or Alexa Fluor 594-conjugated second antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA) for 1 h at RT. Sections and cultures were mixed with the 2nd antibody to assess the total number of cells, and images were acquired as described elsewhere.24 (link)
Zhuang G.Z., Upadhyay U., Tong X., Kang Y., Erasso D.M., Fu E.S., Sarantopoulos K.D., Martin E.R., Wiltshire T., Diatchenko L., Smith S.B., Maixner W, & Levitt R.C. (2018). Human Carbonic Anhydrase-8 AAV8 Gene Therapy Inhibits Nerve Growth Factor Signaling Producing Prolonged Analgesia and Anti-Hyperalgesia in Mice. Gene therapy, 25(4), 297-311.
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3

Antibody-Based Mitochondrial Protein Analysis

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Antibodies directed against the following were used in this study: SLC25A46 (Proteintech, 12277‐1‐AP), MFN2 (Santa Cruz, sc‐100560), SDHA (Abcam, ab14715), AIFM1 (Chemicon, MABN92), ATP5A1 (Abcam, ab14748), HCCS (Proteintech, 15118‐1‐AP), SCO1 (in‐house), MRPL14 (Proteintech, 15040‐1‐AP), NDUFA9 (Abcam, ab14713), UQCRC2 (Abcam, ab14745), COX4 (in‐house), COX2 (in‐house), MFN1 (Cell Signaling, 14739), IMMT/MIC60 (Abnova, H00010989‐M01), CHCHD3/MIC19 (Aviva, ARP57040‐P050), OPA1 (BD biosciences, 612607), MFF (Sigma, SAB1305258), CDKN1A/p21 (Cell Signaling, 2946), p53 (Abcam, ab176243), p‐Mdm2 (Cell Signaling, 3521), LAMP1 (Santa Cruz, sc‐18821), VIM (BD biosciences, 550513), VDAC1 (Abcam, ab14734), EMC1 (GeneTex, GTX119884), EMC2 (ProteinTech, 25443‐1‐AP) and FlagM2 (Sigma, F1804), TOMM20 (Santa Cruz, sc‐11415), DRP1 (BD biosciences, 611113), p637‐DRP1 (Cell Signaling, 6319), p616‐DRP1 (Cell Signaling, 3455), KDEL (Abcam, ab50601), ITPR1 (Cell Signaling, 8568), alpha‐tubulin (Santa Cruz, sc‐23948).
Janer A., Prudent J., Paupe V., Fahiminiya S., Majewski J., Sgarioto N., Des Rosiers C., Forest A., Lin Z., Gingras A., Mitchell G., McBride H.M, & Shoubridge E.A. (2016). SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome. EMBO Molecular Medicine, 8(9), 1019-1038.
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