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Agilent human exon v3 exon 50mb

Manufactured by Agilent Technologies
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The Agilent Human Exon V3 (Exon 50Mb) is a DNA microarray designed for analyzing human exons. It provides comprehensive coverage of the human exome, covering over 500,000 exons across the genome.

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Lab products found in correlation

Seqcap ez human exome library v3, by Agilent Technologies (2 mentions) Hiseq 2000, by Illumina (2 mentions) Repli g kit, by Qiagen (2 mentions) Sureselect human all exon v4, by Agilent Technologies (2 mentions)

2 protocols using agilent human exon v3 exon 50mb

1

Whole Exome Sequencing of Tumor Relapse

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole exome capture was performed on DNA isolated from 48-paired diagnosis and relapse patient samples and patient-matched germline samples. Germline DNA (lymphocytes) was subjected to whole genome amplification (repli-g kit; Qiagen) in 25 patients due to limited materials (see Supplementary Table 2). To obtain sufficient quantities of DNA from the remaining samples, T cells were expanded ex vivo (detailed under Sample collection and processing). DNA was extracted using standard techniques. Libraries were prepared per manufacturer’s recommendation using NimbleGen SeqCap EZ Human Exome Library v3.0, Agilent Human Exon V3 (Exon 50Mb), or Agilent SureSelect Human All Exon V4 (51 MB; see Supplementary Table 2 for specification of kit use per patient tumor and germline samples) and sequenced at a minimum of 50bp single-read sequencing on a HiSeq 2000 (Illumina, Inc.) to a mean coverage per base of 73X. See Supplementary Table 4 for sequencing statistics.
Li S., Garrett-Bakelman F.E., Chung S.S., Sanders M.A., Hricik T., Rapaport F., Patel J., Dillon R., Vijay P., Brown A.L., Perl A.E., Cannon J., Bullinger L., Luger S., Becker M., Lewis I.D., To L.B., Delwel R., Löwenberg B., Döhner H., Döhner K., Guzman M.L., Hassane D.C., Roboz G.J., Grimwade D., Valk P.J., D’Andrea R.J., Carroll M., Park C.Y., Neuberg D., Levine R., Melnick A.M, & Mason C.E. (2016). Distinct evolution and dynamics of epigenetic and genetic heterogeneity in acute myeloid leukemia. Nature medicine, 22(7), 792-799.
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2

Whole Exome Sequencing of Tumor Relapse

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole exome capture was performed on DNA isolated from 48-paired diagnosis and relapse patient samples and patient-matched germline samples. Germline DNA (lymphocytes) was subjected to whole genome amplification (repli-g kit; Qiagen) in 25 patients due to limited materials (see Supplementary Table 2). To obtain sufficient quantities of DNA from the remaining samples, T cells were expanded ex vivo (detailed under Sample collection and processing). DNA was extracted using standard techniques. Libraries were prepared per manufacturer’s recommendation using NimbleGen SeqCap EZ Human Exome Library v3.0, Agilent Human Exon V3 (Exon 50Mb), or Agilent SureSelect Human All Exon V4 (51 MB; see Supplementary Table 2 for specification of kit use per patient tumor and germline samples) and sequenced at a minimum of 50bp single-read sequencing on a HiSeq 2000 (Illumina, Inc.) to a mean coverage per base of 73X. See Supplementary Table 4 for sequencing statistics.
Li S., Garrett-Bakelman F.E., Chung S.S., Sanders M.A., Hricik T., Rapaport F., Patel J., Dillon R., Vijay P., Brown A.L., Perl A.E., Cannon J., Bullinger L., Luger S., Becker M., Lewis I.D., To L.B., Delwel R., Löwenberg B., Döhner H., Döhner K., Guzman M.L., Hassane D.C., Roboz G.J., Grimwade D., Valk P.J., D’Andrea R.J., Carroll M., Park C.Y., Neuberg D., Levine R., Melnick A.M, & Mason C.E. (2016). Distinct evolution and dynamics of epigenetic and genetic heterogeneity in acute myeloid leukemia. Nature medicine, 22(7), 792-799.
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