Anti e cadherin clone 24e10

Manufactured by Cell Signaling Technology

Sourced in United States, United Kingdom

Anti-E-cadherin (clone 24E10) is a rabbit monoclonal antibody that recognizes the extracellular domain of E-cadherin protein. This antibody is suitable for immunohistochemistry and western blotting applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti lamin b c 20, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase conjugated secondary antibody, by GE Healthcare (1 mentions) Anti β actin clone ac 74, by Merck Group (1 mentions) Anti mdm2 clone smp14 and clone h 221, by Santa Cruz Biotechnology (1 mentions) Anti slug clone c19g7, by Cell Signaling Technology (1 mentions) Anti parp1, by Cell Signaling Technology (1 mentions) Bond polymer refine detection kit, by Leica (1 mentions) Anti ki 67 clone 30 9, by Roche (1 mentions) Tyramide alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Peroxidase dab dako real envision detection system, by Agilent Technologies (1 mentions)

Close spelling variants of this product

E-cadherin (clone 24E10, Anti-E-cadherin (24E10, E-cadherin (24E10) Clone 24E10 Anti-E-cadherin E-cadherin

3 protocols using anti e cadherin clone 24e10

Immunoblotting and Protein Isolation Protocols

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The immunoblotting, isolation of nuclear protein fractions, immunoprecipitation and mice protein extraction procedures were performed as previously described [22 (link)].
The primary antibodies used in this work are as follows: human anti-p53 (clone BP53-12, Novocastra Laboratories, Newcastle Upon Tyne, UK), mouse anti-p53 (clone 1C12, Cell Signaling Technology, Beverly, MA, USA), anti-RPL11 (clone 3A4A7, Invitrogen, Carlsbad, UK), anti-PARP-1 (Cell Signaling Technology), anti-RPS6 (clone C-8, Santa Cruz Biotechnology, CA, USA), anti-RPS14 (clone H-130, Santa Cruz Biotechnology), anti-Mdm2 (clone SMP14 and clone H-221, Santa Cruz Biotechnology), anti-Slug (clone C19G7, Cell Signaling Technology), anti-E-cadherin (clone 24E10, Cell Signaling Technology), anti-c-Myc (clone N-262, Santa Cruz Biotechnology), anti-β-actin (clone AC-74, Sigma-Aldrich), and anti-Lamin B (C-20, Santa Cruz Biotechnology). Horseradish peroxidase-conjugated secondary antibodies were from GE-Healthcare (Milan, Italy).

Brighenti E., Giannone F.A., Fornari F., Onofrillo C., Govoni M., Montanaro L., Treré D, & Derenzini M. (2016). Therapeutic dosages of aspirin counteract the IL-6 induced pro-tumorigenic effects by slowing down the ribosome biogenesis rate. Oncotarget, 7(39), 63226-63241.

+ Open protocol

+ Expand

Multimodal Evaluation of Tissue Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human tissues were fixed in formalin and mouse tissues were fixed in 4% paraformaldehyde for 24 h before paraffin embedding. Immunostaining was performed on 5-μm sections via standardized automated protocols on a Ventana Discovery XT machine with the following antibodies: anti-L1CAM (clone 14.10, BioLegend), anti-Ki67 (clone 30–9, Roche) and anti-E-cadherin (clone 24E10, Cell Signaling). For dual LGR5 FISH and L1CAM immunofluorescence, freshly cut 3-μm paraffin sections were stained with the RNAscope 2.5 LS Brown kit (ACD, 322100) for FISH and the Bond Polymer Refine Detection kit (Leica, DS9800) for immunofluorescence on a Leica Bond RX instrument following routine manufacturer protocol ACD 2.5 DAB and Protocol F, using RNAscope 2.5 LS probe for human LGR5 (ACD, 311028) and anti-L1CAM antibody (clone 14.10, BioLegend) with Tyramide Alexa Fluor 594 (Life Technologies, B40957) instead of the DAB step. An Ultra-High-Def mouse-on-mouse kit (StatLab) was used for mouse L1CAM staining. For Ki67 and L1CAM scoring, stained serial sections with overlapping morphology were aligned with the SIFT algorithm and the extent of immunostaining was scored with ImageJ software. L1CAM staining in Extended Data Fig. 4l,m was scored with ImageJ software. All other immunostaining was visually scored in a blinded fashion.

Ganesh K., Basnet H., Kaygusuz Y., Laughney A.M., He L., Sharma R., O’Rourke K.P., Reuter V.P., Huang Y.H., Turkekul M., Emrah E E.r., Masilionis I., Manova-Todorova K., Weiser M.R., Saltz L.B., Garcia-Aguilar J., Koche R., Lowe S.W., Pe’er D., Shia J, & Massagué J. (2020). L1CAM defines the regenerative origin of metastasis-initiating cells in colorectal cancer. Nature cancer, 1(1), 28-45.

+ Open protocol

+ Expand

Immunohistochemical Analysis of S100P and E-Cadherin

Check if the same lab product or an alternative is used in the 5 most similar protocols

S100P and E-cadherin expressions were assessed by imunnohistochemistry in 5 μm sections of FFPE TMAs following standard protocol. Briefly, slides were deparaffinised and hydrated followed by antigen retrieval performed in a HC-Tek Epitope Retrieval Steamer Set for 40 min in 10 mM citrate buffer, pH 6.0. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 min and primary antibodies (anti-S100P, EPR6143, Abcam, UK and anti-E-Cadherin, Clone 24E10, 3195, Cell Signaling, USA) were incubated overnight. Dako REAL Envision Detection System Peroxidase/DAB+ (DAKO, Denmark) was used for detection and sections were then counterstained with hematoxylin, dehydrated and mounted. An experienced pathologist (FS) performed grading of staining, and cases were dichotomized using a simplified classification scheme: retaining (graded as 3+) versus loss (0 to 2+), or positive (from 1+ to 3+) versus negative (graded as 0).

Carneiro P., Moreira A.M., Figueiredo J., Barros R., Oliveira P., Fernandes M.S., Ferro A., Almeida R., Oliveira C., Carneiro F., Schmitt F., Paredes J., Velho S, & Seruca R. (2019). S100P is a molecular determinant of E-cadherin function in gastric cancer. Cell Communication and Signaling : CCS, 17, 155.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!