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Anti grp94

Manufactured by Enzo Life Sciences
Sourced in United States

Anti-GRP94 is a specific antibody that binds to the GRP94 protein. GRP94 is a chaperone protein that assists in the folding and maturation of proteins within the endoplasmic reticulum. This antibody can be used to detect and quantify GRP94 levels in various biological samples.

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2 protocols using anti grp94

1

Western Blot Analysis of Cellular Stress Markers

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Protein extraction and western blot analysis were performed as previously described [52 (link)]. Anti-Beclin-1, anti-cleaved caspase 7, anti-CHOP, anti-GRP78, anti-phospho-H2AX, anti-LC3, anti-PARP, and anti-p62 were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-GRP94 was obtained from Enzo Life Sciences (Farmingdale, NY, USA). Anti-α-tubulin was obtained from Abcam (Eugene, OR, USA).
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2

Antibody Immunoblotting and Immunofluorescence

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Primary antibodies used were anti-viperin (MaP.VIP) (Wang et al., 2007 (link)), anti-GRP94 (Enzo), anti-GAPDH (Proteintech), anti-phospho-4EBP1 (Cell Signaling), anti-4EBP1 (Cell Signaling), anti-PRS6 (Cell Signaling), anti-phospho-RPS6 (Cell Signaling; 2217), anti-phospho-eIF2α (S51) (Cell Signaling), anti-eIF2α (Cell Signaling), anti-Flaviviral E protein (Millipore), anti-GCN2 (Cell Signaling), anti-phospho-GCN2 (T899) (Abcam), anti-PERK (Cell Signaling), anti-HRI (Proteintech), anti-PKR (Cell Signaling), anti-ZAK (Bethyl). All secondary antibodies used for immunofluorescence imaging (goat antimouse IgG coupled to Alexa Fluor 488, Alexa Fluor 647, goat anti-rat IgG coupled to Alexa Fluor 546, Alexa Fluor 594 and goat anti-rabbit IgG coupled to Alexa Fluor 647) were purchased from Invitrogen, and those for immunoblotting (goat anti-mouse IgG and anti-rabbit and anti-rat IgG coupled to horseradish peroxidase and alkaline phosphatase) were purchased from Jackson ImmunoResearch.
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