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Espectra max 2

Manufactured by Molecular Devices
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The Espectra Max 2 is a multi-mode microplate reader designed for diverse scientific applications. It offers precise absorbance, fluorescence, and luminescence detection capabilities. The instrument provides reliable performance and flexibility to support a wide range of experiments.

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Lab products found in correlation

S7903, by Merck Group (2 mentions) N1630, by Merck Group (2 mentions) H3410, by Merck Group (2 mentions) P8250, by Molecular Devices (2 mentions)

4 protocols using espectra max 2

1

NADPH Oxidase Activity Assay

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Nicotinamide adenine dinucleotide phosphate oxidase (NADPH) oxidase was determined by the rate of NADPH consumption assessed by measuring the decline in absorbance (340 nm) every 10 min, using a plate reader spectrophotometer (Espectra Max 2, Molecular Devices) (Wei et al., 2006 (link)). For the assay, we used a 50 mM phosphate buffer containing EDTA (2 mM, Nuclear, 311737), sucrose (150 mM, Sigma–Aldrich Corporation, S7903), NADPH (1.3 mM, Sigma–Aldrich Corporation, N1630), and 10 μL of sample.
Araujo G.S., Silva T.O., Guerra G.M., Izaias J.E., Rocha H.M., Faria D., Rocha N.G., Dalmazo A.L., Araujo A., Marciano Consolim-Colombo F., de Angelis K., Irigoyen M.C, & Sales A.R. (2022). Effects of Postprandial Lipemia Combined With Disturbed Blood Flow on the Flow-Mediated Dilation, Oxidative Stress, and Endothelial Microvesicles in Healthy Subjects. Frontiers in Physiology, 13, 812942.
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2

Spectrophotometric Quantification of H2O2

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Hydrogen Peroxide (H2O2) was analyzed by measuring oxidation of phenol red (Sigma–Aldrich Corporation, H3410) mediated by radish peroxidase (Sigma–Aldrich Corporation, P8250), using a plate reader spectrophotometer (610 nm, Espectra Max 2, Molecular Devices) (Pick and Keisari, 1980 (link)).
Araujo G.S., Silva T.O., Guerra G.M., Izaias J.E., Rocha H.M., Faria D., Rocha N.G., Dalmazo A.L., Araujo A., Marciano Consolim-Colombo F., de Angelis K., Irigoyen M.C, & Sales A.R. (2022). Effects of Postprandial Lipemia Combined With Disturbed Blood Flow on the Flow-Mediated Dilation, Oxidative Stress, and Endothelial Microvesicles in Healthy Subjects. Frontiers in Physiology, 13, 812942.
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3

Peroxidase-based Phenol Red Oxidation

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H2O2 was analyzed through measuring of oxidation of phenol red (Sigma–Aldrich Corporation, H3410) mediated by radish peroxidase (Sigma–Aldrich Corporation, P8250), using a plate reader spectrophotometer (610 nm, Espectra Max 2, Molecular Devices) (Pick and Keisari, 1980 (link)).
Fetter C., Marques J.R., de Souza L.A., Dartora D.R., Eibel B., Boll L.F., Goldmeier S.N., Dias D., De Angelis K, & Irigoyen M.C. (2020). Additional Improvement of Respiratory Technique on Vascular Function in Hypertensive Postmenopausal Women Following Yoga or Stretching Video Classes: The YOGINI Study. Frontiers in Physiology, 11, 898.
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4

NADPH Oxidase Activity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nicotinamide adenine dinucleotide phosphate oxidase (NADPH) oxidase was determined by the rate of NADPH consumption assessed by measuring the decline in absorbance (340 nm) every 10 min, using a plate reader spectrophotometer (Espectra Max 2, Molecular Devices) (Wei et al., 2006 (link)). For the assay, we used a 50 mM phosphate buffer containing EDTA (2 mM, Nuclear, 311737), sucrose (150 mM, Sigma–Aldrich Corporation, S7903), NADPH (1.3 mM, Sigma–Aldrich Corporation, N1630) and 10 μL of sample.
Fetter C., Marques J.R., de Souza L.A., Dartora D.R., Eibel B., Boll L.F., Goldmeier S.N., Dias D., De Angelis K, & Irigoyen M.C. (2020). Additional Improvement of Respiratory Technique on Vascular Function in Hypertensive Postmenopausal Women Following Yoga or Stretching Video Classes: The YOGINI Study. Frontiers in Physiology, 11, 898.
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