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96 well solid white polystyrene microplates

Manufactured by Corning
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Sourced in United States

The 96-well solid white polystyrene microplates are a laboratory equipment product designed for use in various scientific applications. These microplates provide a standardized format with 96 individual wells, allowing for the simultaneous processing of multiple samples or reactions. The white polystyrene material is durable and provides a solid, non-transparent surface for various assays and experiments.

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Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions) Instinct software, by Promega (1 mentions) Glomax multi detection system, by Promega (1 mentions) Celltiter glo luminescent cell viability assay, by Promega (1 mentions) Bright glo luciferase assay, by Promega (1 mentions) Nano glo luciferase assay, by Promega (1 mentions) Luciferase assay system, by Promega (1 mentions) Mgso4, by Avantor (1 mentions) Infinite m200 pro microplate reader, by Tecan (1 mentions)

Spelling variants of this product

96-well microplates (290 citations) 96-well polystyrene microplate (29 citations) Polystyrene microplates (10 citations) 96 wells (10 citations) 96-well white polystyrene microplate (5 citations) 96 Well Solid Polystyrene Microplate (2 citations) Corning 96-well Solid White Flat Bottom Polystyrene microplates (2 citations)

3 protocols using 96 well solid white polystyrene microplates

1

Luciferase Reporter Assay for miRNA

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293 T cells were seeded at 2 × 10 4 cells per well in 96-well solid white polystyrene microplates (Corning, USA). After 16 h, cells were co-transfected with miR-181a-5p mimic and pmirGLO-WT-3’UTR or pmirGLO-MUT-3’UTR using Lipofectamine 3000 (Thermo Fisher Scientific, USA) for 48 h according to the manufacturer’s instructions. The luciferase activity was detected with the Dual-Luciferase Reporter Assay System (Promega, USA). The relative levels of luciferase activity were normalized to the constitutively expressed Renilla.
Wang X.Y., Guan X.H., Yu Z.P., Wu J., Huang Q.M., Deng K.Y, & Xin H.B. (2021). Human amniotic stem cells-derived exosmal miR-181a-5p and miR-199a inhibit melanogenesis and promote melanosome degradation in skin hyperpigmentation, respectively. Stem Cell Research & Therapy, 12, 501.
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2

Luciferase Assay for Cell Viability

Cited 1 time
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The luciferase assay was performed as described previously [23 (link)]. Cells were singularized using Accutase and collected by centrifugation. The cells were resuspended to the equivalent of 100,000 cells/100 µL DMEM/F12 of which 100 µl was placed in 96-Well Solid White Polystyrene Microplates (Corning). Luciferase and Nano-Luc activity were quantified via the Bright-Glo Luciferase Assay (Promega) and Nano-Glo Luciferase Assay (Promega), respectively, according to the manufacturer’s instructions. A CellTiter-Glo Luminescent Cell Viability Assay (Promega) was used to normalize luciferase signal to total cell number. Plates were read on a GloMax®-Multi+ Detection System with Instinct® Software (Promega).
Hsiao C., Lampe M., Nillasithanukroh S., Han W., Lian X, & Palecek S.P. (2016). Human pluripotent stem cell culture density modulates YAP signaling. Biotechnology journal, 11(5), 662-675.
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3

Luciferase Assay for HEK293 Cells

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HEK293 cells were incubated for 15 h after transfection. Cells were lysed and luciferase activity was assayed in the lysates following the recommendations for the Luciferase Assay System (Promega, United States) using a 96-well solid white polystyrene microplates (Corning, United States) and an Infinite M200 PRO microplate reader (Tecan, Switzerland). The substrate solution contained 1 mM D-luciferin from Photinus pyralis (Promega, United States), 25 mM Tris–phosphate, 50 mM 2-mercaptoethanol, 2.5 mM ethylenediaminetetraacetic acid (EDTA), 10 mM MgSO4, (Amresco, United States), and 4 mM deoxyadenosine triphosphate (AppliChem, Germany), pH 7.8.
Selina P.I., Karaseva M.A., Komissarov A.A., Safina D.R., Lunina N.A., Roschina M.P., Sverdlov E.D., Demidyuk I.V, & Kostrov S.V. (2021). Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos. Scientific Reports, 11, 18196.
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