DC phenotype was determined by flow cytometry using a series of antibodies (Abs) including FITC-conjugated anti-CD80, anti-CD83 and PE-conjugated anti-CD86, anti-HLA-DR (BioLegend). The staining followed the same basic procedure. Briefly, imDCs and mDCs were collected and stained with Abs for 30 minutes at 4°C. After washing twice with PBS, the cells were analyzed immediately using a FACSCalibur flow cytometry (Becton Dickinson, Franklin Lakes, NJ, USA).
Fitc conjugated anti cd80
Manufactured by BioLegend
Sourced in United States
FITC-conjugated anti-CD80 is a monoclonal antibody that binds to the CD80 (also known as B7-1) protein expressed on the surface of antigen-presenting cells, such as B cells, dendritic cells, and macrophages. The antibody is conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate), which allows for the detection and analysis of CD80-expressing cells using flow cytometry.
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Lab products found in correlation
2 protocols using fitc conjugated anti cd80
1
Dendritic Cell Phenotyping by Flow Cytometry
2
Phenotyping THP-1 Monocytes and Macrophages
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PE-conjugated anti-CD11b (BioLegend, San Diego, USA) was used as a difference marker between THP-1 cell line monocytes and differentiated MQs. MQs were stained with FITC-conjugated anti-CD80 (BioLegend, San Diego, USA) and PerCP-eFlour710 conjugated anti-CD206 (BD bioscience, USA) antibodies to evaluate the percentages of M1 and M2 MQs, respectively. The duplicate data were analyzed by FlowJo software v10 (FlowJo LCC, Ashland, OR, USA).
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