Nel840001kt

Manufactured by Akoya Biosciences

The NEL840001KT is a laboratory equipment product offered by Akoya Biosciences. It serves as a core function within the research workflow, but a detailed and unbiased description cannot be provided while maintaining conciseness.

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Lab products found in correlation

Phenochart, by Akoya Biosciences (2 mentions) Vectra polaris automated quantitative pathology imaging system, by Akoya Biosciences (1 mentions)

2 protocols using nel840001kt

Multiplexed Immunophenotyping of Murine Samples

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The murine-specific kit from Akoya (NEL840001KT) was used following the manufacturer’s instructions with additional markers. The kit includes the Alexa Fluor Tyramides Opals 520, 570, and 690, and spectral 4’,6-diamidino-2-phenylindole (DAPI). Opals 540 (FP1494001KT), 620 (FP1495001KT), and 650 (FP1496001KT) were from Akoya. Primary antibodies (Cell Signaling) and experimental details are found in online supplemental table 1. Sample scanning, spectral unmixing, and quantification of signals were conducted with Vectra Polaris Automated Quantitative Pathology Imaging System using the Phenochart and InForm V.2.4 softwares (Akoya). Data were given as number of cells with a specific immunophenotype/total number of cells.

Anfray C., Mainini F., Digifico E., Maeda A., Sironi M., Erreni M., Anselmo A., Ummarino A., Gandoy S., Expósito F., Redrado M., Serrano D., Calvo A., Martens M., Bravo S., Mantovani A., Allavena P, & Andón F.T. (2021). Intratumoral combination therapy with poly(I:C) and resiquimod synergistically triggers tumor-associated macrophages for effective systemic antitumoral immunity. Journal for Immunotherapy of Cancer, 9(9), e002408.

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Multispectral Immunophenotyping of Murine Samples

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For multispectral immunophenotyping, the murine-specific kit from Akoya (NEL840001KT) was used as previously described (45 (link)), with additional markers that were evaluated in two separate panels. In the first panel, the following primary antibodies were used: anti-FOXP3, anti-CD4 and anti-CD8; the second panel included: anti-F4/80, anti-CD86 and anti-Arginase-1. The source and dilution of the antibodies as well as other experimental details can be found in Supplementary Table S4. Sample scanning, spectral unmixing and quantification of signals were conducted with the Vectra Polaris Automated Quantitative Pathology Imaging System (Akoya), using the Phenochart (Akoya) and QuPath-0.4.3 software. Data were given as number of cells with a specific immunophenotype/total number of cells.

Anfray C., Varela C.F., Ummarino A., Maeda A., Sironi M., Gandoy S., Brea J., Loza M.I., León S., Calvo A., Correa J., Fernandez-Megia E., Alonso M.J., Allavena P., Crecente-Campo J, & Andón F.T. (2024). Polymeric nanocapsules loaded with poly(I:C) and resiquimod to reprogram tumor-associated macrophages for the treatment of solid tumors. Frontiers in Immunology, 14, 1334800.

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