Gluox

Manufactured by Merck Group

Sourced in United States

GluOX is a laboratory instrument designed for the measurement of glucose levels. It is a compact and efficient device that utilizes electrochemical sensing technology to provide accurate and reliable glucose readings.

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Lab products found in correlation

4 protocols using gluox

Super-Resolution STORM Imaging Protocol

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STORM imaging was performed as described (Sunwoo et al., 2015 (link)). Briefly, cells were imaged on an N-STORM (Nikon) equipped with 100×/1.4 N.A. λ objective lens, ion X3 EM CCD camera (Andor), and 647 nm, 561 nm, and 405 nm lasers. Imaging buffer contained 147 mM βME and GluOX (Sigma). For 3D STORM imaging, cylindrical lens was inserted to the optical path to introduce astigmatism. 3D Z calibration was performed using 100 nm TetraSpeck beads (Life Technology). N-STORM module in Element software (Nikon) was used to control microscopes, acquire images, and perform 2D and 3D STORM localizations.

Kundu S., Ji F., Sunwoo H., Jain G., Lee J.T., Sadreyev R.I., Dekker J, & Kingston R.E. (2017). Polycomb Repressive Complex 1 generates discrete compacted domains that change during differentiation. Molecular cell, 65(3), 432-446.e5.

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Single-Molecule Localization Microscopy

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The dSTORM experiments were conducted in a GLOX switching buffer [39 (link)] and the sample was mounted onto a microscope slide. The imaging buffer is an aqueous solution diluted in PBS containing an enzymatic oxygen scavenging system GluOx (2000 U ml⁻¹ glucose-oxidase (Sigma Aldrich, catalog number: G2133-50KU), 40 000 U ml⁻¹ catalase (Sigma Aldrich, catalog number: C100), 25 mM potassium chloride (Sigma Aldrich, catalog number: 204439), 22 mM tris (hydroxymethyl) aminomethane (Sigma-Aldrich, catalog number: T5941), 4 mM tris (2-carboxyethyl) phosphine (TCEP) (Sigma-Aldrich, catalog number: C4706) with 4% (w/v) glucose (Sigma Aldrich, catalog number: 49139) and 100 mM β-mercaptoethylamine (MEA) (Sigma-Aldrich, catalog number: M6500). The final pH was set to 7.4.

Brunner S., Varga D., Bozó R., Polanek R., Tőkés T., Szabó E.R., Molnár R., Gémes N., Szebeni G.J., Puskás L.G., Erdélyi M, & Hideghéty K. (2021). Analysis of Ionizing Radiation Induced DNA Damage by Superresolution dSTORM Microscopy. Pathology and Oncology Research, 27, 1609971.

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3D STORM Imaging Protocol

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STORM imaging was performed as previously described (Sunwoo et al., 2015 (link)) on an N-STORM (Nikon) equipped with 100x/1.4 N.A. λ objective lens, ion X3 EM CCD camera (Andor), and 3 laser lines (647 nm, 561 nm, and 405 nm). A cylindrical lens was inserted into the optical path to introduce astigmatism. Z calibration was performed using 100 nm TetraSpeck beads (Life Technologies). Imaging buffer containing 147 mM βME and GluOX (Sigma) was used to promote blinking and reduce photo-bleaching. Z calibration was performed using 100 nm TetraSpeck beads. N-STORM module in Element software (Nikon) was used to control microscopes, acquire images, and perform 3D STORM localizations.

Colognori D., Sunwoo H., Kriz A., Wang C.Y, & Lee J.T. (2019). Xist deletional analysis reveals a co-dependency between Xist RNA and Polycomb complexes for spreading along the inactive X. Molecular cell, 74(1), 101-117.e10.

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Electrochemical Detection of Glutamate Using Chitosan

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GluOx (Glutamate oxidase) (EC 1.4.3.11, from Streptomyces sp) and Glutamate were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Potassium phosphate, KH₂PO_4, and diPotassium phosphate K₂HPO₄ were obtained from Merck Ltd. They used to prepare phosphate buffer solution as a supporting electrolyte in all electrochemical experiments. Chitosan (high molecular weight, MW∼1000 kDa; ∼80% deacetylation) was obtained from the Aldrich Company. All aqueous solutions were prepared in double-distilled water with a resistance of 18.0 MΩ cm⁻¹. Double-distilled deionized water was prepared using an ion-exchange system (Millipore, France). Cyclic voltammetry and chronoamperometry experiments were carried out using Galvanostat/Potentiostat apparatus (Autolab 302 N, Holland) by a conventional three-electrode system at controlled temperature conditions.

Faraji F., Tavakoli H., Jafari M., Eidi A, & Divsalar A. (2023). Electrochemical study of the effect of radiofrequency on glutamate oxidase activity using a glutamate oxidase-based biosensor. Heliyon, 9(5), e15911.

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