The following antibodies were used in this study: biotin-anti-Lineage (TER-119, RB6-8C5, RA3-6B2, M1/70, 145-2C11, 1:50 dilution), FITC/PE-anti-CD45.2 (104), FITC/PE/PE-Cy7-anti-Ly6A/E (D7), BV421/PE/PE-Cy7-anti-CD117 (2B8), biotin-anti-CD48 (HM48-1), biotin-anti-CD41 (MWReg30, 1:2500 dilution), APC-Cy7-anti-CD45 (30-F11), Pe-anti-CD51 (RMV-7), Alexa647-anti-VE-cadherin (BV13) APC-anti-CD31 (MEC13.3), FITC/PE/PE-Cy7-anti-CD45.1 (A20), PE-anti-CD150 (TC15-12F12.2), CD41-PerCP-eFluor 710 (MWReg30), Gr-1-FITC/Alexa 647 RB6-8C5), CD4-PE-Cy7 (GK1.5), CD8-PE-Cy7 (53-6.7), CD115-PE (AFS98), APC-eflour780 (RA3-6B2) all from eBioscience. CD16/32-APC-Cy7 (93), CD105-PE-Cy7 (MJ7/18), Ter119-Alexa 700 (Ter 119), F4/80-Alexa 647 (BM8,) were from Biolegend. BrdU-Alexa 647 (3D4, BD Biosciences, 1:50 dilution), Alexa 647-anti-Ki67 (SolA 15) and Hoechst 33342 (Sigma). Unless otherwise specified, all antibodies were used at a 1:100 dilution.
Apc cy7 anti cd45
Manufactured by Thermo Fisher Scientific
APC-Cy7-anti-CD45 is a fluorescently labeled antibody that binds to the CD45 antigen expressed on the surface of human leukocytes. It is used in flow cytometry applications for the identification and enumeration of various immune cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Rb6 8c5, by Thermo Fisher Scientific (2 mentions)
Fitc pe pe cy7 anti ly6a e d, by Thermo Fisher Scientific (2 mentions)
Bv421 pe pe cy7 anti cd117 2, by Thermo Fisher Scientific (2 mentions)
Biotin anti cd48, by Thermo Fisher Scientific (2 mentions)
Biotin anti cd41, by Thermo Fisher Scientific (2 mentions)
Pe anti cd51, by Thermo Fisher Scientific (2 mentions)
Alexa647 anti ve cadherin bv13 apc anti cd31, by Thermo Fisher Scientific (2 mentions)
Fitc pe pe cy7 anti cd45, by Thermo Fisher Scientific (2 mentions)
Pe anti cd150, by Thermo Fisher Scientific (2 mentions)
Cd41 percp efluor 710, by Thermo Fisher Scientific (2 mentions)
Brdu alexa 647 3d4, by BD (2 mentions)
Alexa 647 anti ki67 sola 15, by Merck Group (2 mentions)
Hoechst 33342, by Merck Group (2 mentions)
Ra3 6b2, by Thermo Fisher Scientific (2 mentions)
Pe anti cd163, by Thermo Fisher Scientific (1 mentions)
Percp5.5 anti cd4, by Thermo Fisher Scientific (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Anti cd3 fitc, by Thermo Fisher Scientific (1 mentions)
Anti cd8 apc, by Thermo Fisher Scientific (1 mentions)
Anti cd206 pe, by Thermo Fisher Scientific (1 mentions)
5 protocols using apc cy7 anti cd45
1
Multicolor Flow Cytometry Antibodies
2
Comprehensive Flow Cytometry Analysis of Tumor Cells and Immune Populations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The markers of human tumor cells, as well as those of human and mouse TAMs and T cells were determined by flow cytometry after surface or intracellular staining with specific antibodies conjugated with different fluorescence. For intracellular staining of IFN-γ, erythrocyte-excluded blood and spleen cells were first incubated with Cell Stimulation Cocktail plus protein transport inhibitors (eBioscience; Cat No. 4975-03) at 37 °C under 5% CO2 for 5 h. The following human or mouse antibodies were used: PE-anti-ILT4, PE-anti-CD163, PE-anti-CD80, PE-anti-CD86, PE-anti-CD206, APC/CY7-anti-CD45, Percp5.5-anti-F4/80, FITC-anti-CD3, Percp5.5-anti-CD4, APC-anti-CD8, and PE-anti-IFN-γ. All the antibodies were purchased from Biolegend. The stained cells were analyzed on a FACS Calibur flow cytometer (BD Bioscience) and data were analyzed using FlowJo10 software (Tree Star, Inc.; Ashland; OR).
3
Multicolor Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Flow cytometry was performed on BAL and lung cells. Cells were incubated for 15 minutes with the CD16/CD32 Fc block antibody and then stained with extracellular antibodies (BD Biosciences unless otherwise stated) for antigen-presenting cells (APC-Cy7-anti-CD45, Alexa Fluor 700-anti-MHCII (eBioscience, San Diego, CA), Pacific Orange-anti-Gr1 (Life Technologies, Burlington, Ontario), APC-anti-CD11c, PECy7-anti-CD11b), NK cells (V450-anti-CD3, PE-anti-NK1.1), and T cells (V450-anti-CD3, APC-Cy7-anti-CD4). Stained cells were analyzed on the LSRII flow cytometer (BD Biosciences) and analyzed using FlowJo software (Tree Star, Ashland, OR). Neutrophils (CD45+CD11b+Gr1+), macrophages (CD45+Gr1-AF+CD11c+), NK cells (CD3-NK1.1+), and CD4+ T cells (CD3+CD4+) were assessed as total number of cells per tissue compartment by multiplying the “frequency of total” of the population by total cell number in the tissue.
4
Multicolor Flow Cytometry Antibodies
5
Quantifying Murine Macrophage Subsets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The proportion of macrophage in the spleen of mice or BMDMs were analyzed by flow cytometry. These cells were stained with APC-CY7-anti-CD45, Fitc-anti-CD11b, PE-anti-F4/80, PE-CY7-anti-CD86, APC-anti-CD206, and their isotype controls (eBioscience, CA) according to the protocol. Flow cytometry was performed with the FACSCalibur (BD Immunocytometry Systems). All data were analyzed using FlowJo software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!