Surgery was performed under aseptic conditions with isoflurane anesthesia. Infusions were made using a 33-gauge beveled needle (World Precision Instruments) and stereotaxic injector (Stoelting). For rats in the NAc shell-VP disconnection experiments, rats received unilateral infusions of an inhibitory DREADD (AAV8-hSyn-Gi-hM4Di-mCitrine; UNC vector core) into the NAc shell (1.0 μl) and VP (0.8 μl) in either opposite hemispheres (Group Contra; n = 14) or the same hemisphere (Group Ipsi; n =12). Coordinates for NAc shell infusions were 1.4 mm anterior of bregma, 0.8 mm from the midline, and 7.2 mm ventral from the skull surface. Coordinates for VP infusions were 0.12 mm anterior of bregma, 2.4 mm from the midline, and 8.2 mm ventral from the skull surface. The location of infusions (i.e., left or right hemisphere) was counterbalanced for each group. For the NAc shell experiment, rats received bilateral infusions (1.0 μl) of either an inhibitory DREADD (same as above; Group Gi; n = 7) or a green fluorescent protein (AAV8-hSyn-GFP; UNC vector core). The coordinates for NAc shell infusions were the same as above. All infusions were made at a rate of 0.15 μl/min. Transgene expression was allowed to take place for 3 weeks before the start of behavioral training.
Stereotaxic injector
Manufactured by Stoelting
Sourced in United States
The Stereotaxic Injector is a precision instrument designed for accurate and controlled delivery of micro-volumes of liquids or solutions into targeted regions of the brain or other tissues. It provides reliable and consistent injection parameters to support various research and experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
33 gauge beveled needle, by World Precision Instruments (1 mentions)
Stereotactic frame, by Kopf Instruments (1 mentions)
Celebrex, by Pfizer (1 mentions)
Abatacept, by Bristol-Myers Squibb (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
Hamilton syringe, by Hamilton Company (1 mentions)
Ketamine 1000, by Ceva (1 mentions)
Domitor, by Pfizer (1 mentions)
Xylocain, by AstraZeneca (1 mentions)
7 protocols using stereotaxic injector
1
Inhibitory DREADD Manipulation of NAc-VP Circuitry
2
Intracranial Glioma Models and Immunotherapy
3
Orthotopic Brain Tumor Xenograft Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal procedures were approved by the Johns Hopkins University Animal Care and Use Committee. Prior to transplantation, GBM1, GBM551, and DIPG neurospheres were harvested, dissociated into single cells, and suspended in phosphate buffered saline (PBS) at a final concentration of 1 × 105/μL. The total 2 × 105 cells were loaded into a 10-μL Hamilton syringe with an attached 31-gauge needle and injected at a rate of 1 μL/min into the right striatum (anteroposterior = 1.0 mm; mediolateral = 2.0 mm; dorsoventral = 2.5 mm) of C57BL/6 J mice (8–10 weeks, n = 5 for each cell line) and SCID mice (8–10 weeks, n = 5 for each cell line, The Jackson Laboratory) through a stereotaxic injector (Stoelting). After the injection was complete, the needle was kept in place for 2 minutes and then withdrawn to avoid backflow of the injected cells through the needle tract.
For T-cell costimulatory blockade experiments, hamster anti-mouse CD154mAb (MR1, BioXcell) and CTLA-4-Ig (Abatacept, Bristol-Myers Squibb) were administered to C57BL/6 J mice (500 μg each) intraperitoneally (i.p.) on d 0, 2, 4, and 6 after tumor inoculation. Mice were monitored twice a week for weight changes and neurological symptoms.
For T-cell costimulatory blockade experiments, hamster anti-mouse CD154mAb (MR1, BioXcell) and CTLA-4-Ig (Abatacept, Bristol-Myers Squibb) were administered to C57BL/6 J mice (500 μg each) intraperitoneally (i.p.) on d 0, 2, 4, and 6 after tumor inoculation. Mice were monitored twice a week for weight changes and neurological symptoms.
4
Retrograde Labeling of Cochlear Root Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To label CRNs and the cochlear nerve, we used identical procedures to those used in our previous studies (Gómez-Nieto et al., 2008b (link); Gómez-Nieto and Rubio, 2009 (link)). Three animals received a unilateral injection of the fluorescent tracer, dextran fluorescein isothiocyanate (D-FITC; #D-1820; Molecular Probes, Eugene, OR), into the left TB to label CRNs retrogradely. The stereotaxic coordinates targeted the course of CRN axons which project to PnC neurons via the TB (López et al., 1999 (link)). A volume of 0.15–0.2μl of D-FITC (10% in distilled water) were pressure-delivered with a Hamilton syringe (#710; Hamilton Co., Reno, NV) attached to a Stereotaxic Injector (Stoelting Co., Wood Dale, IL). After the scalp was sutured, the animals were allowed to recover for 4–7 days. Then, the animals were deeply anesthetized and perfused through the heart with 0.5% paraformaldehyde (PFA) in 0.1 M phosphate buffer, pH 7.4. The cochlea was removed, and crystals of the lipophylic dye DiI (#D-3911; 1.1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate; Molecular Probes) were placed on the exposed auditory nerve. The brains were stored in 4% PFA for approximately 30 days at room temperature protected from light and were processed for confocal microscopy as described by Gómez-Nieto and Rubio (2009 (link)).
5
Targeted Gene Silencing in Rat Dentate Gyrus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On PND 44–45, rats received bilateral microinjections of either AAV-control or AAV-αCAMKII-kd virus vector in the dDG. 1 μl of viral vector suspension was injected in each hemisphere (0.15 μl/min) through a 10 μl Hamilton syringe (30G) connected to a motorized nanoinjector (Stereotaxic Injector, Stoelting, Wood Dale, USA) (Saha et al., 2018 (link); Tripathi et al., 2021 (link)).
6
S100B Striatal Injection in Rodents
7
Stereotactic Injection of Cells in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were conducted according to the European Union Community Council guidelines and were approved by the Animal Ethics Committee of the KU Leuven. Before surgery, animals were anaesthetized by an intraperitoneal injection with a mixture of ketamine (Ketamine1000, 4.5 mg/kg; Ceva, Pompidou, France)/medetomidin (Domitor®, 0.6 mg/kg; Pfizer, New York, USA). Local analgesia (2 % xylocain; AstraZeneca, London, UK) and antibiotics (6 mg/mouse, Ampiveto-20, 200 mg/ml; VMD, New Haw, Surrey, UK) were administered prior to surgery. After fixation of the animals in a stereotactic frame adapted with a stereotaxic injector (both from Stoelting, Wood Dale, USA), cells were suspended in 5 μl phosphate-buffered saline (PBS) and injected (0.5 μl/min) with a 10 μl Hamilton syringe, equipped with a 22 G needle, into the right striatum of C57Bl6/j mice at the following coordinates: 0.5 mm anterior and 2.0 mm lateral to bregma at 3.0 mm from the dura.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!