The brain, heart, liver, spleen, lung, stomach, intestine, testicles and kidney were promptly collected after the mice of the homing experiments (n=5) were sacrificed and fixed in a 10% formalin solution. Then, the organs were embedded in paraffin, sectioned, and processed for H&E staining and pathologically assessed for histological abnormality or toxicity by a neuropathologist. IHC was carried out on 3 μm brain tumor tissues of different groups (n=3 per group) and probed using rabbit anti-human CD3 (Abcam).
Rabbit anti human cd3
Manufactured by Abcam
Sourced in United Kingdom
Rabbit anti-human CD3 is a primary antibody that targets the CD3 complex on the surface of T cells. It can be used to identify and quantify T cells in various research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using rabbit anti human cd3
1
Histopathological Evaluation of Organ Toxicity
2
Histopathological Evaluation of Organ Toxicity
3
In Vivo Evaluation of CAR-T Cell Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The heart, liver, spleen, lungs, kidneys, and tumor masses were dissected. All dissected mice were sacrificed 39 days after subcutaneous tumor seeding. Tissue specimens were fixed with 4% buffered formaldehyde, and paraffin sections were analyzed using hematoxylin-eosin staining and immunohistochemistry. H&E staining of major organs was used for the preliminary safety evaluation of CAR-T cells in vivo, and immunohistochemical staining of the spleen was used to evaluate CAR-T cell persistence and infiltration. The primary and secondary antibodies used in this study were rabbit anti-human CD3 and hrp-labeled goat anti-rabbit IgG H&L (Abcam, Cambridgeshire, GB, UK), respectively, followed by DAPI staining (Abcam, Cambridgeshire, GB, UK) for nuclear staining.
4
Immunofluorescence Analysis of Cell Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh frozen sections were blocked with 3% BSA, and then, rabbit antiphospho-S6 ribosomal protein (Ser 235/236) antibody (Cell Signaling Technology), combined with mouse antihuman CD31 (Santa Cruz) or goat antihuman synaptopodin (Santa Cruz), rabbit antihuman CD3 (Abcam) combined with mouse antihuman CD4 and CD8 (Abcam) and interleukin (IL)-17A conjugated with PE (BD Biosciences) were added and incubated overnight at 4°C, followed by the secondary antibodies Alexa Fluor 488-labelled donkey antirabbit IgG, Alexa Fluor 647-labelled donkey antimouse IgG (Abcam) or TRITC-labelled donkey antigoat IgG (Invitrogen) for 30 min at 37°C. Nuclei were stained with 4′,6-diamidino-2-phenylindole (ZSGB-Bio). For negative controls, primary antibodies were replaced by PBS. Fluorescence images were acquired with fluorescence microscopy (DM2500; Leica, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!