Protein a ultralink resin

Manufactured by Thermo Fisher Scientific

Protein A Ultralink resin is a chromatography resin designed for the purification of antibodies and antibody-containing samples. It is composed of protein A, a bacterial protein, immobilized on an agarose-based matrix. The resin provides a high-capacity, high-affinity binding platform for the capture and isolation of immunoglobulins, making it a versatile tool for researchers and biopharmaceutical manufacturers.

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Lab products found in correlation

X vivo 15 medium, by Lonza (1 mentions)

Close spelling variants of this product

Pierce Protein A/G Ultralink Resin Protein A/G UltraLink Resin Protein A/G UltraLink Resin beads Protein A Plus Ultralink resin Protein A resin

3 protocols using protein a ultralink resin

Immunoprecipitation of GFP-tagged Proteins

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Human Embryonic Kidney (HEK) 293T cells were lysed 48 h following transfection in immunoprecipitation buffer containing: 20 mM Tris HCL, 100 mM NaCl, 1 mM EDTA pH 8.0, and 1% NP-40, supplemented with protease/phosphatase inhibitors (Biotool). Protein extracts were incubated for 2 h with rotation at 4°C with the precipitating antibody (rabbit anti-GFP 1:500) followed by overnight incubation with 25 μl of protein A Ultralink resin (Thermo Scientific). The resin were washed several times with immunoprecipitation buffer and precipitated proteins were eluted by boiling for 5 min in reducing SDS sample buffer and resolved by SDS-PAGE.

Dillon G.M., Tyler W.A., Omuro K.C., Kambouris J., Tyminski C., Henry S., Haydar T.F., Beffert U, & Ho A. (2017). CLASP2 Links Reelin To The Cytoskeleton During Neocortical Development. Neuron, 93(6), 1344-1358.e5.

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Antibody Purification from B Cell Clones

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For antibody purification, B cell clones were expanded in X-VIVO 15 medium (Lonza, Verviers, Belgium). MAbs were purified from the pooled supernatants using Protein A Ultralink Resin (Thermo Fisher Scientific) and elution in Gentle Ab/Ag Elution Buffer (Thermo Fisher Scientific), according to the manufacturer’s instructions. Purified mAbs were dialyzed overnight against PBS and sterilized by 0.2 µM filtration (Meran, Istanbul, Turkey).

Valgardsdottir R., Cattaneo I., Napolitano G., Raglio A., Spinelli O., Salmoiraghi S., Castilletti C., Lapa D., Capobianchi M.R., Farina C, & Golay J. (2021). Identification of Human SARS-CoV-2 Monoclonal Antibodies from Convalescent Patients Using EBV Immortalization. Antibodies, 10(3), 26.

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HEK293T Protein Immunoprecipitation

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Transfected HEK293T cells were lysed, and protein extracts were incubated with precipitating antibody and protein A Ultralink resin (Thermo Scientific). Precipitated proteins were resolved by SDS-PAGE and immunoblotted for APP, GFP, and α-tubulin.

Bartling C.R., Jensen T.M., Henry S.M., Colliander A.L., Sereikaite V., Wenzler M., Jain P., Maric H.M., Harpsøe K., Pedersen S.W., Clemmensen L.S., Haugaard-Kedström L.M., Gloriam D.E., Ho A, & Strømgaard K. (2021). Targeting the APP-Mint2 Protein–Protein Interaction with a Peptide-Based Inhibitor Reduces Amyloid‑β Formation. Journal of the American Chemical Society, 143(2), 891-901.

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