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Anti kip2 p57 polyclonal antibody

Manufactured by Santa Cruz Biotechnology
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The Anti-Kip2 p57 polyclonal antibody is a laboratory reagent used to detect the presence and expression levels of the p57 protein, a member of the Kip family of cyclin-dependent kinase inhibitors. This antibody can be used in various immunoassay techniques to analyze the p57 protein in biological samples.

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Diaminobenzidine dab, by Thermo Fisher Scientific (2 mentions)

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Anti-p57 (5 citations)

2 protocols using anti kip2 p57 polyclonal antibody

1

Quantifying Podocyte Count via p57 Staining

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Podocyte number was measured by quantitating p57 staining. Indirect immunoperoxidase staining for p57 was performed on formalin-fixed biopsies as previously reported in detail24 (link)–26 (link),29 (link),30 (link) with a rabbit anti-Kip2 p57 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA). Diaminobenzidine (DAB) was detected as a brown color (Fisher). For podocyte number, the number of cells in the glomerular tuft staining for p57 was measured on 25–30 glomeruli per cross section. Because of potential differences in glomerular tuft area with treatment, the glomerular tuft area was measured on periodic acid-Schiff (PAS)-stained slides, and used as a denominator to assess podocyte number.
Zhang J., Yanez D., Floege A., Lichtnekert J., Krofft R.D., Liu Z.H., Pippin J.W, & Shankland S.J. (2014). ACE-inhibition increases podocyte number in experimental glomerular disease independent of proliferation. Journal of the renin-angiotensin-aldosterone system : JRAAS, 16(2), 234-248.
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2

Quantifying Podocyte Count via p57 Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Podocyte number was measured by quantitating p57 staining. Indirect immunoperoxidase staining for p57 was performed on formalin-fixed biopsies as previously reported in detail24 (link)–26 (link),29 (link),30 (link) with a rabbit anti-Kip2 p57 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA). Diaminobenzidine (DAB) was detected as a brown color (Fisher). For podocyte number, the number of cells in the glomerular tuft staining for p57 was measured on 25–30 glomeruli per cross section. Because of potential differences in glomerular tuft area with treatment, the glomerular tuft area was measured on periodic acid-Schiff (PAS)-stained slides, and used as a denominator to assess podocyte number.
Zhang J., Yanez D., Floege A., Lichtnekert J., Krofft R.D., Liu Z.H., Pippin J.W, & Shankland S.J. (2014). ACE-inhibition increases podocyte number in experimental glomerular disease independent of proliferation. Journal of the renin-angiotensin-aldosterone system : JRAAS, 16(2), 234-248.
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