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Advia counter

Manufactured by Siemens
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The Advia Counter is a laboratory equipment product manufactured by Siemens. It is designed to perform automated blood cell counting and analysis. The core function of the Advia Counter is to provide accurate and reliable measurements of various blood cell parameters, such as red blood cells, white blood cells, and platelets.

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Lab products found in correlation

Hemavet 950, by Drew Scientific (2 mentions) Micro haematocrit capillary tubes, by Thermo Fisher Scientific (2 mentions) 0.5m edta, by Thermo Fisher Scientific (1 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)

4 protocols using advia counter

1

Blood Cell Enumeration and Preparation

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Blood was collected from the facial vein in tubes containing EDTA. White blood cell (WBC), red blood cell (RBC) and platelet counts were obtained using an Advia Counter (Siemens) or a Hemavet 950 (Drew Scientific). Prior to flow cytometry staining and analyses, red blood cells in peripheral blood were lysed once by adding 1 mL of RBC Lysis Buffer (NH4Cl 150mM, NaCO3 10mM, EDTA 0.1mM). Cells were immediately decanted by centrifugation, resuspended in ice-cold PEB and used in subsequent assays.
Bowers E., Slaughter A., Frenette P.S., Kuick R., Pello O.M, & Lucas D. (2017). Granulocyte-derived TNFα promotes vascular and hematopoietic regeneration in the bone marrow. Nature medicine, 24(1), 95-102.
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2

Blood Cell Enumeration and Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood was collected from the facial vein in tubes containing EDTA. White blood cell (WBC), red blood cell (RBC) and platelet counts were obtained using an Advia Counter (Siemens) or a Hemavet 950 (Drew Scientific). Prior to flow cytometry staining and analyses, red blood cells in peripheral blood were lysed once by adding 1 mL of RBC Lysis Buffer (NH4Cl 150mM, NaCO3 10mM, EDTA 0.1mM). Cells were immediately decanted by centrifugation, resuspended in ice-cold PEB and used in subsequent assays.
Bowers E., Slaughter A., Frenette P.S., Kuick R., Pello O.M, & Lucas D. (2017). Granulocyte-derived TNFα promotes vascular and hematopoietic regeneration in the bone marrow. Nature medicine, 24(1), 95-102.
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3

Blood Sampling in Mice under Anesthesia

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Mice were bled ~25 μl into an Eppendorf tube containing 2 μl of 0.5 M EDTA (Life Technologies) using heparinised micro-haematocrit capillary tubes (Fisherbrand) under isoflurane anaesthesia. Blood was diluted 1:20 in PBS and analysed on an Advia counter (Siemens).
Wei Q., Nakahara F., Asada N., Zhang D., Gao X., Xu C., Alfieri A., Brodin N.P., Zimmerman S.E., Mar J.C., Guha C., Guo W, & Frenette P.S. (2020). Snai2 maintains bone marrow niche cells by repressing osteopontin expression. Developmental cell, 53(5), 503-513.e5.
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4

Mice Blood Sampling and Analysis

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Mice were bled ~25 μl into an Eppendorf tube containing 2 μl of 0.5 M EDTA (Life Technologies) using heparinised micro-haematocrit capillary tubes (Fisherbrand) under isoflurane anaesthesia. Blood was diluted 1:20 in PBS and analysed on an Advia counter (Siemens).
Wei Q., Pinho S., Dong S., Pierce H., Li H., Nakahara F., Xu J., Xu C., Boulais P.E., Zhang D., Maryanovich M., Cuervo A.M, & Frenette P.S. (2021). MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance of haematopoietic stem cells. Nature Communications, 12, 2522.
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