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3 protocols using chlorogenic acid

1

Reagents for Phytochemical Analysis

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All reagents were obtained from Sigma Aldrich (Milan, Italy), with the following exceptions: solvents, protocatechuic acid, isoorientin, orientin, isovitexin, chlorogenic acid and rutin were purchased from VWR International s.r.l. (Milan, Italy); p-Coumaric acid was purchased from Fluka (Steinheim, Germany); caffeic acid was procured from Extrasynthèse (Lyon, France).
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2

Extraction and Characterization of Phenolic Compounds

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Acetone, acetonitrile, and formic acid (purity > 99.9%) were purchased from Sigma Aldrich (Amsterdam, Holland); hydrochloric acid was purchased from Carlo Erba (Milan, Italy). The standard solutions (purity > 99.9%) at 1,000 mg L−1 of gallic acid, catechin, caffeic acid, syringic acid, rutin, ellagic, hesperidin, ferulic acid, myricetin, quercetin, apigenin, naringenin and kaempferol were purchased from Sigma-Aldrich S. r.l. (Milan, Italy); chlorogenic acid was purchased from VWR (Milan, Italy). apigenin and kaempferol were dissolved in aqueous solution at pH > 8.
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3

Rapid Synthesis of Antioxidant-Enriched Sensors

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Magnesium nitrate hexahydrate (Mg(NO 3 ) 2 •6H 2 O), aluminum nitrate nonahydrate (Al(NO 3 ) 3 •6H 2 O), sodium hydroxide (NaOH) and lactic acid (85%) were purchased from Merck and used without purification. Copper(II) chloride dihydrate (CuCl 2 •2H 2 O), neocuproine (Nc), trolox, L(+)-ascorbic acid, eugenol, diosmin, gallic acid, glutathione (reduced), catechin hydrate, tannic acid, chlorogenic acid ( predominantly trans), sodium salicylate, sodium alginate (NaAlg), sodium chloride (NaCl) and ethanol were obtained from VWR International in analytical grade and used as received. For sensor preparation, cellulose filter paper (Whatman Grade 602H, VWR) and crayon were used. Ultrapure water was obtained from a Puranity TU3+ UV/UF system.
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