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2 deoxythymidine

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2'-deoxythymidine is a chemical compound that is commonly used as a reagent in laboratory research. It is a pyrimidine nucleoside that consists of the nucleobase thymine attached to a deoxyribose sugar. 2'-deoxythymidine is a key component in the synthesis of DNA and is often used in various biochemical and molecular biology applications, such as DNA sequencing, DNA labeling, and nucleic acid amplification techniques.

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Lab products found in correlation

2 deoxycytidine, by Merck Group (3 mentions) 2 deoxyadenosine, by Merck Group (2 mentions) 2 deoxyguanosine, by Merck Group (2 mentions) 5 methyl 2 deoxycytidine, by Merck Group (1 mentions) Adenosine, by Fujifilm (1 mentions) Uridine, by Tokyo Chemical Industry (1 mentions) Cytidine, by Tokyo Chemical Industry (1 mentions) Bacterial alkaline phosphatase type 3 e coli, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions) 2 3 dideoxyinosine, by Merck Group (1 mentions) Benzo a pyrene, by Fujifilm (1 mentions) Guanosine, by Tokyo Chemical Industry (1 mentions) Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Pierce quantitative colorimetric peptide assay, by Thermo Fisher Scientific (1 mentions) Cell lysis solution, by Qiagen (1 mentions) Goat anti rabbit igg hrp secondary antibody, by Santa Cruz Biotechnology (1 mentions) Protein precipitation solution, by Qiagen (1 mentions) Glacial acetic acid, by Thermo Fisher Scientific (1 mentions) Oxiselect 8 iso prostaglandin f2α elisa kit, by Cell Biolabs (1 mentions)

4 protocols using 2 deoxythymidine

1

Quantitative DNA Methylation Analysis

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The extent of methylation in DNAs was determined by HPLC method (Kuo et al., 1980 (link)) and performed with the reverse-phase HPLC system (Agilent1260, Wilmington, MA, United States) with UV detection at 280 nm, using the Agilent HC-C18 column (250 mm × 4.6 mm dimension) at a flow rate of 1 mL/min with a mobile phase of 50 mM KH2PO4: methanol at 90: 10 (v/v). DNA methylation was quantified with the nucleoside standards (2′-deoxyadenosine, 5′-methyl-2′-deoxycytidine, 2′-deoxycytidine, 2′-deoxyguanosine, and 2′-deoxythymidine) purchased from Sigma (St. Louis, MO, United States).
Ma Y.J., Lu C.S, & Wang J.W. (2018). Effects of 5-Azacytidine on Growth and Hypocrellin Production of Shiraia bambusicola. Frontiers in Microbiology, 9, 2508.
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2

Nuclease and Phosphatase Enzyme Purchases

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Bovine spleen phosphodiesterase II (SPD) and micrococcal nuclease (MN) were purchased from Worthington Biochemical Corp. (Lakewood, NJ, USA). Bacterial alkaline phosphatase Type III (E. coli), 2′-deoxycytidine, 2′-deoxythymidine, 2′-deoxyadenosine, 2′-deoxyGuanosine and 2′,3′-dideoxyinosine were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Benzo[a]pyrene (>98%), adenosine (>98%), phosphate buffered saline(-) [PBS(-)], methanol (LC/MS grade), and dimethyl sulfoxide (DMSO) were purchased from Wako Chemical (Osaka, Japan). Guanosine (>98%), cytidine (>98%) and uridine (>98%) were purchased from Tokyo Chemical Industry Co (Tokyo, Japan).
Takeshita T, & Kanaly R.A. (2019). In vitro DNA/RNA Adductomics to Confirm DNA Damage Caused by Benzo[a]pyrene in the Hep G2 Cell Line. Frontiers in Chemistry, 7, 491.
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3

Synchronizing Jurkat Cell Cycle

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Cellular replication of E6.1 Jurkat cells was synchronized using a single thymidine block protocol to arrest cells between late G1 and early S-phase. Briefly, cells (1 million cells/ml) were treated with thymidine (2′-deoxythymidine, Sigma) at a final concentration of 1 mM and incubated for 18 h. To promote release into the S-phase, the block was alleviated by two media changes and addition of cytidine (2′-deoxycytidine, Sigma) to a final concentration of 50 µM. Cell cycle progression was assessed by flow cytometry every two hours over a 12-h period by analysing cellular DNA content following cell fixing in ethanol and staining with propidium iodide (PI) (Sigma). This approach was used to detect cells in each of the three major phases of the cycle (G0/G1, S and G2/M), and also allowed for detection of apoptotic cells with minimal DNA content.
Seddon A.R., Liau Y., Pace P.E., Miller A.L., Das A.B., Kennedy M.A., Hampton M.B, & Stevens A.J. (2021). Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells. Epigenetics & Chromatin, 14, 17.
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4

Quantitative Proteomic Analysis of Oxidative Stress

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2′-deoxythymidine, Boc-L-Tyr-OH, trifluoroacetic acid, Nuclease P1 from Penicillium citrinum, and DL-dithiothreitol were purchased from Sigma-Aldrich (St. Louis, MO). Pierce™ BCA Protein Assay kit, Pierce™ Quantitative Colorimetric Peptide Assay, Pierce Quantitative Colorimetric Peptide Assay, and TMT 6-plex reagents were purchased from Thermo Fischer Scientific (Waltham, MA). Glacial acetic acid, hydrochloric acid, and acetonitrile were obtained from Fisher Scientific (Buchs, Switzerland). Mass spectrometry grade Trypsin Gold was purchased from Promega (Madison, WI). Cell lysis solution, protein precipitation solution, and RNaseA were purchased from Qiagen (Hilden, Germany). Amicon 3K filters were purchased from Millapore (Darmstadt, Germany). Omega Nanosep 10K Omega filters were obtained from PALL Life Science (Port Washington, NY). The OxiSelect™ 8-iso-prostaglandin F2α ELISA kit was purchased from Cell Biolabs (San Diego, CA). Matrin-3 and glyceraldehyde-3-phosphate dehydrogenase primary antibody and goat anti-rabbit IgG-HRP secondary antibody were purchased from Santa Cruz Biotechnology (Dallas, TX). Superoxide dismutase [Mn] mitochondrial and pyruvate dehydrogenase E1 beta subunit primary antibody were purchased from Abcam (Cambridge, UK).
Groehler A I.V., Kren S., Li Q., Robledo-Villafane M., Schmidt J., Garry M, & Tretyakova N. (2018). Oxidative Cross-Linking of Proteins to DNA Following Ischemia-Reperfusion Injury. Free radical biology & medicine, 120, 89-101.
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