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Mueller hinton broth (mhb)

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Mueller Hinton Broth is a laboratory medium used for the cultivation and testing of microorganisms. It is a general-purpose broth formulated to support the growth of a wide range of bacteria and fungi. The broth provides essential nutrients and growth factors required for microbial proliferation.

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2 protocols using mueller hinton broth (mhb)

1

Peptide Synthesis and Microbial Assays

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Mueller-Hinton, Agar SPC, Mueller Hinton Broth (MHB), E. coli ATCC 11775, S. maltophilia ATCC 13636, and S. enteritidis ATCC 13076 were obtained from ATCC (Manassas, VA, USA). BLF, N,N-diisopropylethylamine (DIPEA), triisopropylsilane (TIPS), 1,2-ethanedithiol (EDT), 4-methylpiperidine, pyridine, and ninhydrin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Rink amide resin, Fmoc-amino acids, 6-chloro-1-hydroxy-benzotriazole (6-Cl-HOBt), and N,N-dicyclohexylcarbodiimide (DCC) were purchased from AAPPTec (Louisville, KY, USA). Methanol, diethyl ether, N,N-dimethylformamide (DMF), absolute ethanol, dichloromethane (DCM), acetonitrile (ACN), isopropylalcohol (IPA), and trifluoroacetic acid (TFA) were obtained from Honeywell-Burdick & Jackson (Muskegon, MI, USA). All reagents were used without further purification.
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2

Peptide Synthesis Using Standard Reagents

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Mueller-Hinton, Agar SPC, Mueller Hinton Broth (MHB), E. coli ATCC 11775 and E. coli ATCC 25,922 were obtained from ATCC (Manassas, VA, USA), Fetal Bovine Serum (FBS) was obtained of Gibco. N,N-diisopropylethylamine (DIPEA), triisopropylsilane (TIPS), 1,2-ethanedithiol (EDT), 4-methylpiperidine, pyridine, and ninhydrin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Rink amide resin, Fmoc-amino acids, 6-chloro-1-hydroxy-benzotriazole (6-Cl-HOBt), and N,N-dicyclohexylcarbodiimide (DCC) were purchased from AAPPTec (Louisville, KY, USA). Methanol, diethyl ether, N,N-dimethylformamide (DMF), absolute ethanol, dichloromethane (DCM), acetonitrile (ACN), isopropylalcohol (IPA), and trifluoroacetic acid (TFA) were obtained from Honeywell-Burdick & Jackson (Muskegon, MI, USA). All reagents were used without further purification.
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