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Goat anti mouse po

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Goat anti-Mouse-PO is a secondary antibody conjugated with peroxidase (PO). It is designed to detect and bind to mouse primary antibodies in immunoassays and other applications.

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Lab products found in correlation

Goat anti rabbit po, by Agilent Technologies (6 mentions) Hsp90, by Santa Cruz Biotechnology (4 mentions) Ab992, by Abcam (2 mentions) Ab128873, by Abcam (2 mentions) Smc1a, by Fortis Life Sciences (1 mentions) Igg rabbit, by Merck Group (1 mentions) Anti p21, by Abcam (1 mentions) Anti smc3, by Abcam (1 mentions) Anti acetyl p53, by Abcam (1 mentions) Anti sirt1, by Cell Signaling Technology (1 mentions) Anti p53, by Cell Signaling Technology (1 mentions) Anti hsp90 f 8, by Santa Cruz Biotechnology (1 mentions) Anti cd71 fitc, by Southern Biotech (1 mentions) Jbw301, by Merck Group (1 mentions) Anti hp1γ, by Merck Group (1 mentions) Anti gapdh 6c5, by Abcam (1 mentions) Goat anti human alexa647, by Thermo Fisher Scientific (1 mentions) Anti β actin, by Merck Group (1 mentions)

6 protocols using goat anti mouse po

1

Antibody Validation for Protein Analysis

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Western Blots were performed using the following antibodies: WAPL (Santa Cruz, A-7), SCC4 (Abcam, ab46906), SCC2 N-terminal (Santa Cruz, C-9), SCC2 C-terminal (Absea, serum of KT55), HSP90 (Santa Cruz, H-114), CDK4 (Santa Cruz, C-22) and Actin (Santa Cruz, I-19). All antibodies were used at 1:1000 dilution. Secondary antibodies Goat anti-Rabbit-PO, Goat anti-Mouse-PO, Rabbit anti-Goat-PO (DAKO) and Rabbit anti-Rat-PO (Santa Cruz, sc2006) were used at 1:2000 dilution. For immunofluorescence we used the SCC1 (Millipore, 05-908) antibody in a 1:100 dilution. CTCF (Millipore, 07-72), SMC1A (Bethyl, A300-055a) and IgG Rabbit (Sigma, I5006) were used for chromatin immuno-precipitations.
Haarhuis J.H., van der Weide R.H., Blomen V.A., Yáñez-Cuna J.O., Amendola M., van Ruiten M.S., Krijger P.H., Teunissen H., Medema R.H., van Steensel B., Brummelkamp T.R., de Wit E, & Rowland B.D. (2017). The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension. Cell, 169(4), 693-707.e14.
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2

Western Blot and ChIP-seq Antibodies

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The following antibodies were used for western blots: SMC1 (A300-055A, Bethyl), CTCF (07-729, Millipore) and ab128873, Abcam), HSP90 (F-8, Santa Cruz), SCC1 (05-908, Millipore) (Tubulin (T5168, Sigma), H4 (05-858, Millipore). All primary antibodies were used at a 1:1000 dilution, with the exception of HSP90 and Tubulin (1:10.000). Secondary antibodies for western blot analysis were used in a 1:2000 dilution: Goat anti-Rabbit-PO and Goat anti-Mouse-PO (DAKO). For ChIPseq we used the following antibodies: SCC1 (ab992, Abcam), CTCF (3418S, Cell Signaling) and IgG (I5006, Sigma-Aldrich).
Li Y., Haarhuis J.H., Cacciatore Á.S., Oldenkamp R., van Ruiten M.S., Willems L., Teunissen H., Muir K.W., de Wit E., Rowland B.D, & Panne D. (2020). The structural basis for cohesin-CTCF anchored loops. Nature, 578(7795), 472-476.
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3

Coimmunoprecipitation and Immunoblot Analyses

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Coimmunoprecipitation experiments were performed with the following antibodies: SMC1 (Bethyl, no. A300-055A) and SCC2 (Bethyl, no. A301-779A). Immunoblots were performed using the following antibodies and dilutions: HSP90 (Santa Cruz, no. sc13119 F8, 1:10,000), ESCO1 (a kind gift from S. Rankin37 (link), 1:1,500), HDAC8 (Sigma-Aldrich, no. WH0055869M1, 1:1,000), AcSMC3 (a kind gift from K. Shirahige38 (link), 1:1,500), WAPL (Santa Cruz, no. sc365189, 1:1,000), SMC1 (Bethyl, no. A300-055A, 1:2,000), SMC3 (Bethyl, no. A300-060A-5, 1:2,000), SCC1 (Millipore, no. 05-908, 1:1,000), PDS5A (Bethyl, no. A300-089A, 1:1,000), PDS5B (Bethyl, no. A300-538A, 1:500), SCC2 (Santa Cruz, no. sc374625, 1:1,000), SCC4 (Abcam, no. ab46906, 1:1,000), Actin (Abcam, no. ab6276, 1:5,000) and Tubulin (Abcam, no. ab18251, 1:10.000). Secondary antibodies Goat-anti-Mouse-PO (DAKO, no. P0447) and Goat-anti-Rabbit-PO (DAKO, no. P0448) were used at 1:2,000 dilution.
van Ruiten M.S., van Gent D., Sedeño Cacciatore Á., Fauster A., Willems L., Hekkelman M.L., Hoekman L., Altelaar M., Haarhuis J.H., Brummelkamp T.R., de Wit E, & Rowland B.D. (2022). The cohesin acetylation cycle controls chromatin loop length through a PDS5A brake mechanism. Nature Structural & Molecular Biology, 29(6), 586-591.
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4

Western Blot and ChIP-seq Antibodies

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The following antibodies were used for western blots: SMC1 (A300-055A, Bethyl), CTCF (07-729, Millipore) and ab128873, Abcam), HSP90 (F-8, Santa Cruz), SCC1 (05-908, Millipore) (Tubulin (T5168, Sigma), H4 (05-858, Millipore). All primary antibodies were used at a 1:1000 dilution, with the exception of HSP90 and Tubulin (1:10.000). Secondary antibodies for western blot analysis were used in a 1:2000 dilution: Goat anti-Rabbit-PO and Goat anti-Mouse-PO (DAKO). For ChIPseq we used the following antibodies: SCC1 (ab992, Abcam), CTCF (3418S, Cell Signaling) and IgG (I5006, Sigma-Aldrich).
Li Y., Haarhuis J.H., Cacciatore Á.S., Oldenkamp R., van Ruiten M.S., Willems L., Teunissen H., Muir K.W., de Wit E., Rowland B.D, & Panne D. (2020). The structural basis for cohesin-CTCF anchored loops. Nature, 578(7795), 472-476.
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5

Western Blot Antibody Validation

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The following antibodies were used for western blots: WAPL (A-7, sc-365189, Santa Cruz), HSP90 (F-8, sc-13119, Santa Cruz), MED12 (A300-774A, Bethyl), CCNC (ab85927, Abcam), CTCF (ab70303, Abcam), Actin (ab6276, Abcam) and Tubulin (T5168, Sigma-Aldrich). All primary antibodies were used at a 1:1000 dilution, except for Tubulin 1:4000. Secondary antibodies for western blot analysis were used in a 1:2000 dilution: Goat anti-Rabbit-PO and Goat anti-Mouse-PO (DAKO P0448 and P0447, respectively).
Haarhuis J.H., van der Weide R.H., Blomen V.A., Flach K.D., Teunissen H., Willems L., Brummelkamp T.R., Rowland B.D, & de Wit E. (2022). A Mediator-cohesin axis controls heterochromatin domain formation. Nature Communications, 13, 754.
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6

Comprehensive Antibody List for Cell Analysis

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The following antibodies were used: anti-CD71-FITC (SouthernBiotech, 1720-02, 0.5 mg ml−1, 1:500)20 (link), anti-SIRT1 (rabbit, Cell Signalling, 2496S, 1:1,000), anti-centromere (Antibodies, 15-234-0001, 1:1,000), anti-rabbit Alexa 488 (Thermo Fisher Scientific, A11034, 1:2,000), goat anti-human Alexa 647 (Thermo Fisher Scientific, A21445, 1:2,000), anti-DSCC1 (H0079075-B01P, Novus Biologicals, 1:1,000), anti-HSP90 (F-8, Santa Cruz, 1:10,000), anti-HP1γ (05-690, Millipore, 1:1,000), goat-anti-mouse-PO (DAKO, P044701, 1:2,000), anti-SMC3 (Abcam, AB 9263, 1:250), anti-SMC3 (Thermo Fisher Scientific, A300-060A, 1:1,000), anti-acetyl SMC3 mouse (Sigma-Aldrich, MABE1073, 21A7, Lys105/106, 385016, 1:1,000), anti-p53 (Cell Signaling Technology, 1C12, 2524S), anti-acetyl p53 (p53-K382Ac, Abcam, ab75754, EPR358(2) to p53 acetyl K382, 1:1,000), anti-phosphorylated-histone H2A.X (Ser139) (JBW301, Sigma-Aldrich, 05-636-I, 1:1,000), anti-β-actin (Merck, A5441, 1:10,000, 5% milk), anti-GAPDH (6C5, Abcam, ab8245, 1:1,000), anti-p21 (Abcam, ab109520, 1:1,000). Uncropped western blots are provided in the Supplementary Information.
Adams D.J., Barlas B., McIntyre R.E., Salguero I., van der Weyden L., Barros A., Vicente J.R., Karimpour N., Haider A., Ranzani M., Turner G., Thompson N.A., Harle V., Olvera-León R., Robles-Espinoza C.D., Speak A.O., Geisler N., Weninger W.J., Geyer S.H., Hewinson J., Karp N.A., Fu B., Yang F., Kozik Z., Choudhary J., Yu L., van Ruiten M.S., Rowland B.D., Lelliott C.J., del Castillo Velasco-Herrera M., Verstraten R., Bruckner L., Henssen A.G., Rooimans M.A., de Lange J., Mohun T.J., Arends M.J., Kentistou K.A., Coelho P.A., Zhao Y., Zecchini H., Perry J.R., Jackson S.P, & Balmus G. (2024). Genetic determinants of micronucleus formation in vivo. Nature, 627(8002), 130-136.
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