Vitamin D3 (Vit D, Cholecalciferol), Kolliphor®P188 (Poloxamer 188, F-68), Tween®20 (T20), and Tween®80 (T80) were purchased (Sigma-Aldrich, USA). The cross-linked hyaluronic acid (HA, 20 mg/ml) hydrogel with 1,4-butanediol diglycidyl ether (BDDE) was provided (Hyundae Meditech Co., Ltd., Korea).
Tween 20 t20
Manufactured by Merck Group
Sourced in United States, Germany, Canada
Tween-20 (T20) is a non-ionic detergent commonly used in various laboratory applications. It is a polyoxyethylene (20) sorbitan monolaurate, a type of surfactant. Tween-20 is a clear, viscous liquid that is soluble in water and many organic solvents. It is widely used in biochemical and molecular biology techniques, such as Western blotting, ELISA, and protein purification, to solubilize and stabilize proteins, and to reduce non-specific binding in immunoassays.
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Lab products found in correlation
4 protocols using tween 20 t20
1
Vitamin D3 Hydrogel Formulation
2
Omega Oil Encapsulation Formulation
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The refined fish oil (Omega Oil 1812 TG Gold) was purchased from BASF Personal Care and Nutrition GmbH (Illertissen, Germany) and stored at −80 °C until use. The Tween-20 (T20) was obtained from Sigma-Aldrich (Darmstadt, Germany) and CITREM (GRINDSTED® CITREM LR 10 EXTRA MT) were provided by Danisco (Copenhagen, Denmark). The pullulan (P) was kindly donated by Hayashibara Co., Ltd. (Okayama, Japan). The glucose syrup (GS; DE38, C*Dry 1934) was supplied by Cargill Germany GmbH (Krefeld, Germany). The maltodextrin (MD; DE21) and whey protein concentrate (ca. 35 wt% protein content) were generously donated by Abbott Laboratories S.A. (Granada, Spain). The whey protein concentrate hydrolysate (WPCH) used as an emulsifier was produced in an automatic titrator (718 Stat Titrino; Metrohm AG, Herisau, Switzerland) using Alcalase 2.4 L to a degree of hydrolysis of 10% (DH10), as described by Rahmani-Manglano et al. [16 (link)]. Then, the hydrolysate (WPCH) was freeze dried and stored at 4 °C until further use. The rest of the reagents used for the analysis were of analytical grade.
3
Polymeric Micelles for Cargo Delivery
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Carboxyl-terminated polystyrene-b-polyethylene oxide (PS-b-PEO-COOH, 9500-b-18,000 Da) and poly (ε-caprolactone)-b-polyethylene oxide (PCL-b-PEO-COOH, 6000-b-5000 Da) BCPs were used for micelle synthesis. PS-b-PEO and PCL-b-PEO were obtained from Polymer Source Inc. (cat. no. P5755-SEOCOOH and P3130-EOCL, respectively). Lipid micelles were formed using a commercial phospholipid, PHOSHPOLIPON 90G (P90G) obtained from Lipoid (PHOSPHOLIPID GmbH, item no. SA-L368202). Lutein (xanthophyll, cat. no. X6250), Coumarin 6 (cat. no. 442,631) (a fluorescent dye reporter), polyvinyl alcohol (PVA, cat. no. 363,170), and Tween 20 (T20, P1379) were purchased from Sigma Aldrich.
4
Microbial Adhesion Characterization Protocol
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MEG-TFA was synthesized according to previously published methods [37 (link)]. Heptane was purchased from Sigma-Aldrich (St. Louis, MO, USA). Polyurethane tubing was purchased from McMaster Carr (Aurora, OH, USA). Ethanol was obtained from Caledon Laboratory Chemicals (Georgetown, ON, Canada). Broths bases, agar, and fetal bovine serum (FBS) were purchased from University of Toronto MedStore (Toronto, ON, Canada). Glutaraldehyde (GDA) and Tween-20 (T20) were purchased from Sigma-Aldrich (Mississauga, ON, Canada). Staining was performed with SYTOX green (Life Technologies, Carlsbad, CA, USA). Adhesion experiments were carried out with Escherichia coli GFP (ATCC 25922GFP) and Staphylococcus aureus KR3.
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