Automated hematology analyzer

Manufactured by Drew Scientific

Sourced in United States

The Automated Hematology Analyzer is a laboratory instrument designed to perform comprehensive analysis of blood samples. It automates the process of counting and classifying different blood cell types, providing accurate and reliable results.

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Lab products found in correlation

8 protocols using automated hematology analyzer

Mouse Platelet Isolation and Preparation

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Platelets were prepared as previously described.14, 15, 22 Mouse blood was collected from a ventricle and the citrated (0.38%) blood was mixed with phosphate‐buffered saline, pH 7.4, and before incubation with prostaglandin I2 (10 ng/mL; 5 minutes) and centrifugation at 237g for 10 minutes at room temperature. Platelet‐rich plasma (PRP) was recovered and platelets were pelleted at 483g for 10 minutes at room temperature. The pellets were resuspended in HEPES/Tyrode buffer (20 mmol/L HEPES/KOH, pH 6.5, 128 mmol/L NaCl, 2.8 mmol/L KCl, 1 mmol/L MgCl₂, 0.4 mmol/L NaH₂PO₄, 12 mmol/L NaHCO₃, 5 mmol/L D‐glucose) supplemented with 1 mmol/L EGTA, 0.37 U/mL apyrase, and 10 ng/mL epoprostenol. Platelets were then washed and resuspended in HEPES/Tyrodes (pH 7.4) without EGTA, apyrase, or epoprostenol. Platelets were counted with an automated hematology analyzer (Drew Scientific, Dallas, TX) and adjusted to the indicated concentrations.

Hernandez K.R., Karim Z.A., Qasim H., Druey K.M., Alshbool F.Z, & Khasawneh F.T. (2019). Regulator of G‐Protein Signaling 16 Is a Negative Modulator of Platelet Function and Thrombosis. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 8(5), e011273.

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Platelet Activation and Signaling Assay

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Serotonin hydrochloride, pizotifen and ADP were obtained from Sigma Aldrich (St. Louis, MO), cyproheptadine and EMD 281014 were obtained from Tocris Bioscience (Bristol, UK), clopidogrel was purchased from LKT Laboratories, Inc. (St. Paul, MN), stir bars and other disposables were from Chrono-Log (Havertown, PA), and U46619 was obtained from Cayman Chemical (Ann Arbor, MI). Src antibody, FITC-conjugated Annexin V, anti–P-selectin, and PAC-1 antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). The anti-phosphotyrosine antibody was from BD Biosciences, (Franklin Lakes, NJ). Fura-2 acetoxymethyl ester (fura-2/AM) and Pluronic® F-127 were from Invitrogen (Grand Island, NY). The C57BL/6 mice were obtained from Jackson laboratory (Bar Harbor, ME). Platelet count was determined using an automated hematology analyzer (Drew Scientific Dallas, TX).

Lin O.A., Karim Z.A., Vemana H.P., Espinosa E.V, & Khasawneh F.T. (2014). The Antidepressant 5-HT2A Receptor Antagonists Pizotifen and Cyproheptadine Inhibit Serotonin-Enhanced Platelet Function. PLoS ONE, 9(1), e87026.

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Isolation and Preparation of Mouse Platelets

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Mouse platelets were prepared, as discussed previously.42, 43 Briefly, mouse blood was collected from a ventricle, and the citrated (0.38%) blood was mixed with PBS, pH 7.4, and was incubated with prostaglandin I₂ (10 ng/mL; 5 minutes), followed by centrifugation at 237g for 10 minutes at room temperature. Platelet‐rich plasma was recovered, and platelets were pelleted at 483g for 10 minutes at room temperature. The pellets were resuspended in HEPES/Tyrode's buffer (20 mmol/L HEPES/KOH, pH 6.5; 128 mmol/L NaCl; 2.8 mmol/L KCl; 1 mmol/L MgCl₂; 0.4 mmol/L NaH₂PO₄; 12 mmol/L NaHCO₃; and 5 mmol/L d‐glucose) supplemented with 1 mmol/L EGTA, 0.37 U/mL apyrase, and 10 ng/mL prostaglandin I₂. Platelets were washed and resuspended in HEPES/Tyrode's buffer (pH 7.4) without EGTA, apyrase, or prostaglandin I₂. Platelets were counted with an automated hematology analyzer (Drew Scientific, Dallas, TX) and adjusted to the indicated concentrations.

Alshbool F.Z., Karim Z.A., Espinosa E.V., Lin O.A, & Khasawneh F.T. (2018). Investigation of a Thromboxane A2 Receptor–Based Vaccine for Managing Thrombogenesis. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 7(13), e009139.

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Isolation and Preparation of Mouse Platelets

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Mouse blood was collected from the ventricle and the citrated (0.38%) blood was mixed with phosphate-buffered saline, pH 7.4, and incubated with PGI₂ (10 ng/mL; 5 min), followed by centrifugation at 237×g for 10 min at room temperature (RT). Platelet-rich plasma (PRP) was recovered and platelets were pelleted at 483×g for 10 min at RT. The pellets were resuspended in HEPES/Tyrode's buffer (HT; 20 mM HEPES/KOH, pH 6.5, 128 mM NaCl, 2.8 mM KCl, 1 mM MgCl₂, 0.4 mM NaH₂PO₄, 12 mM NaHCO₃, 5 mM d-glucose) supplemented with 1 mM EGTA, 0.37 U/mL apyrase, and 10 ng/mL PGI₂. Platelets were washed and resuspended in HT (pH 7.4) without EGTA, apyrase, or PGI₂. Platelets were counted with an automated hematology analyzer (Drew Scientific Dallas, TX) and adjusted to the indicated concentrations.

Paez Espinosa E.V., Lin O.A., Karim Z.A., Alshbool F.Z, & Khasawneh F.T. (2019). Mouse transient receptor potential channel type 6 selectively regulates agonist-induced platelet function. Biochemistry and Biophysics Reports, 20, 100685.

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Platelet Count Measurement by Analyzer

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Platelet count was determined using a automated hematology analyzer (Drew Scientific, Dallas, TX, USA).

Villalobos-García D., Ali H.E., Alarabi A.B., El-Halawany M.S., Alshbool F.Z, & Khasawneh F.T. (2022). Exposure of Mice to Thirdhand Smoke Modulates In Vitro and In Vivo Platelet Responses. International Journal of Molecular Sciences, 23(10), 5595.

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Platelet Isolation and Preparation

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Platelets were prepared as previously described [11 (link)]. Mouse blood was collected from a ventricle and the citrated (0.38%) blood was mixed with phosphate-buffered saline, pH 7.4, and was incubated with PGI₂ (10 ng/mL; 5 min), followed by centrifugation at 237x g for 10 min at room temperature (RT). Platelet-rich plasma (PRP) was recovered and platelets were pelleted at 483x g for 10 min at RT. The pellets were resuspended in HEPES/Tyrode buffer (20 mM HEPES/KOH, pH 6.5, 128 mM NaCl, 2.8 mM KCl, 1 mM MgCl₂, 0.4 mM NaH₂PO₄, 12 mM NaHCO₃, 5 mM D-glucose) supplemented with 1 mM EGTA, 0.37 U/mL apyrase, and 10 ng/mL PGI₂. Platelets were then washed and resuspended in HEPES/Tyrodes (pH 7.4) without EGTA, apyrase, or PGI₂. Platelets were counted with an automated hematology analyzer (Drew Scientific Dallas, TX) and adjusted to the indicated concentrations.

Hensch N.R., Karim Z.A., Druey K.M., Tansey M.G, & Khasawneh F.T. (2016). RGS10 Negatively Regulates Platelet Activation and Thrombogenesis. PLoS ONE, 11(11), e0165984.

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Murine Platelet Signaling Assays

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U46619 was from Cayman Chemical (Ann Arbor, MI). Thrombin receptor activating peptide 4 (TRAP4; AYPGKF-NH2) and ADP analog Adenosine 5′-[β-thio]diphosphate trilithium salt was from Sigma Aldrich (St. Louis, MO). Antibodies for Akt, pAkt, ERK and pERK were from Cell Signaling (Danvers, MA). ADP and other platelet disposables were from Chrono-Log (Havertown, PA). TRPC6 inhibitor (GsMTx-4) was from Alomone labs (Israel). FITC-conjugated Annexin V, anti–P-selectin, and PAC-1 antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Fura-2 acetoxymethyl ester (fura-2/AM) and Pluronic F-127 were from Invitrogen (Grand Island, NY). Sodium citrate, whatman filter paper, ferric chloride, sodium chloride, potassium chloride, sodium dihydrogen phosphate, magnesium chloride, sodium bicarbonate, and D-dextrose were from Fisher Scientific (Hanover Park, IL). The radiolabeled [³H]SQ29,548 was purchased from pERKinElmer (Waltham, MA). BAPTA was purchased from Tocris Bioscience (Ellisville, Missouri).The C57BL/6 mice were obtained from Jackson laboratory (Bar Harbor, ME). Platelet count was determined using an automated hematology analyzer (Drew Scientific Dallas, TX).

Vemana H.P., Karim Z.A., Conlon C, & Khasawneh F.T. (2015). A Critical Role for the Transient Receptor Potential Channel Type 6 in Human Platelet Activation. PLoS ONE, 10(4), e0125764.

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Platelet Count in 5HT2A Receptor Antibody Mice

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Platelet count was determined in mice injected daily with 150 nM of 5HT_2ARAb or vehicle for 7 or 14 days using an automated hematology analyzer (Drew Scientific, Miami Lakes, FL, USA).

Ramirez J.E., Alarabi A.B., Khasawneh F.T, & Alshbool F.Z. (2022). A Novel Antibody Targeting the Second Extracellular Loop of the Serotonin 5-HT2A Receptor Inhibits Platelet Function. International Journal of Molecular Sciences, 23(15), 8794.

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