H&E histology was conducted at 6, 12, 24 and 72 h following reperfusion. Rats were anesthetized by an intraperitoneal injection of 10% chloral hydrate (100 g/0.3 ml) and then perfused transcardially with saline (Beijing Solarbio Science & Technology Co., Ltd.; 250 ml), followed by 4% formaldehyde (Beijing Solarbio Science & Technology Co., Ltd.; 250 ml). Brains were removed and fixed in 4% formaldehyde at 4°C for 72 h and then dehydrated and embedded in paraffin blocks (Beijing Solarbio Science & Technology Co., Ltd.). Coronal sections were cut posterior to the optic chiasma, at a thickness of 3 mm. The sections were deparaffinized and hydrated with reducing concentrations of alcohol, stained with H&E (Beijing Solarbio Science & Technology Co., Ltd.) and photographed using an optical microscope (Axio Scope.A1; Zeiss AG, Oberkochen, Germany).
Formaldehyde
Manufactured by Solarbio
Sourced in China
4% formaldehyde is a laboratory fixative solution used for the preservation and fixation of tissue samples. It is a ready-to-use aqueous solution containing 4% w/v formaldehyde.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Solarbio (9 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions)
Glycine, by Solarbio (2 mentions)
Crystal violet, by Solarbio (2 mentions)
Triton x 100, by Thermo Fisher Scientific (2 mentions)
Cell light edu apollp567 in vitro kit, by RiboBio (2 mentions)
Anti desmin, by Bioss Antibodies (2 mentions)
Bovine serum albumin (bsa), by Solarbio (2 mentions)
Lsm 710, by Zeiss (2 mentions)
Lsm 880 confocal microscope, by Zeiss (2 mentions)
Gradient alcohol, by Sinopharm (1 mentions)
Biomicroscopy, by Olympus (1 mentions)
Masson staining kit, by Solarbio (1 mentions)
Xylene, by Sinopharm (1 mentions)
He staining kit, by Solarbio (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Solarbio (1 mentions)
Crystal violet, by Beyotime (1 mentions)
Acetone, by Solarbio (1 mentions)
Hydrochloric acid (hcl), by Solarbio (1 mentions)
Mgcl2, by Solarbio (1 mentions)
37 protocols using formaldehyde
1
H&E Analysis of Brain Tissue Reperfusion
2
Femoral Head Histological Analysis
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The femoral head was fixed with 4% formaldehyde (Solarbio), decalcified with 10% EDTA (Solarbio), dehydrated with gradient alcohol (SINOPHARM, Beijing, China), made transparent using xylene (SINOPHARM), embedded in paraffin (SINOPHARM), and sliced with a Leica pathological slicer (RM2016, Leica, Wetzlar, Germany). The slices were dewaxed in xylene and gradient alcohol in turn. The dewaxed sections were stained according to the HE staining kit (Solarbio) and the Masson staining kit (Solarbio) and then examined under a biomicroscopy (Olympus, Tokyo, Japan).
3
Quantifying Breast Cancer Cell Clonogenicity
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Then 500 transfected BC cells were seeded in 6-well plates. Two weeks later, clones were fixed with 10% formaldehyde (Beijing Solarbio science and Technology Co. Ltd) and stained with 0.1% crystal violet (Beyotime). Clones with diameters greater than 1 mm were measured and counted.
4
Tilapia Protein Extraction Protocol
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Fresh tilapia (500 g ± 100 g) was purchased from the Yonghui supermarket (a local supermarket in Fuzhou, China). Curdlan was purchased from VWR Life Science Amresco Products, Avantor Performance Materials, Inc. Tris, HCl, KCl, NaN3, β‐mercaptoethanol, Mg (CH3COO)2, ethylene glycol‐bis‐(2‐chloroethyl) tetraacetic acid (EGTA), ATPNa2, KHCO3, MgCl2, formaldehyde, glutaraldehyde, phosphoric acid salt buffer, ethanol, and acetone were obtained from Solarbio Science & Technology Co., Ltd. The above reagents were all of analytical grade.
5
Annexin A3 Expression Analysis
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Formaldehyde and Triton X-100 were purchased from Beijing Solarbio Science & Technology Co., Ltd. and diluted to the indicated final concentrations. Anti-ANXA3 (1:1,000; cat. no. ab33068) and mouse monoclonal anti-β-actin antibodies (1:2,000; cat. no. ab8226) were obtained from Abcam. Horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibodies (1:5,000; cat. no. ZB2301) and HRP-conjugated anti-mouse secondary antibodies (1:5,000; cat. no. ZB2305) were obtained from ZSGB-BIO; OriGene Technologies, Inc. ProLong® Diamond Antifade Mountant and 4′, 6-diamidino-2-phenylindole (DAPI) were obtained from Thermo Fisher Scientific, Inc. and the Lipofectamine 2000™ transfection reagent was obtained from Invitrogen; Thermo Fisher Scientific, Inc. The RNAprep pure Cell kit and the Transcriptor First Strand cDNA synthesis kit were purchased from Tiangen Biotech Co., Ltd. The RNA expression profiles of ANXA3 were obtained from The Cancer Genome Atlas (TCGA) database (www.cancer.gov/about-nci/organization/ccg/research/structural-genomics/tcga ). Annexin V-phycoerythrin (PE)/7-aminoactinomycin D (AAD) Apoptosis Detection kit was obtained from BD Biosciences.
6
Quantitative Proteomic Analysis of Doxorubicin-Induced Cellular Changes
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Doxorubicin (Solarbio, Beijing, China), formaldehyde (Solarbio, Beijing, China), heparin sodium (Solarbio, Beijing, China), acetonitrile (Merck, Germany), formic acid (CNW, Germany), BCA Kit (Solarbio, Shangahi, China), Tris (Sigma, USA), SDS (Bio-Rad, USA), phenylmethylsulfonyl fluoride (Solarbio, Beijing), phosphatase inhibitor (Solarbio, Beijing, China), RIPA buffer (Solarbio, Beijing, China), PTP1B (Abcam, USA), IRS-1, P-IRS, HK2, HIF-1α (Cell Signaling Technology, Inc., USA), Nrf2 (Abcam, USA), NH4HCO3 (Sigma, USA), High pH Reversed-Phase Peptide Fractionation Kit (Pierce, USA), TMT 6/10 plex Isobaric Label Reagent (Thermo, USA) were used.
7
Exploring OCT4A Regulation of FTX
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To explore the potential mechanism of OCT4A on FTX expression, the online databases JASPAR (http://jaspar.genereg.net ) and PROMO (http://alggen.lsi.upc.es/cgibin/promo_v3/promo/promoinit.cgi?dirDB=TF_8.3 ) were used to predict the possible binding regions of OCT4A and the FTX promoters. A ChIP Kit (Axl-Biotech, China) was used to perform ChIP assays according to the manufacturer’s instructions. In brief, cells were incubated with 1% formaldehyde (Solarbio, China) for 10 min for DNA–protein cross-link generation. Cell lysates were then collected and sonicated to yield genomic DNA fragments of 200–600 bp and immunoprecipitated with an OCT4A antibody (Abcam, UK). Normal rabbit IgG was used as a control. qPCR was conducted using ChamQ SYBR qPCR Master Mix (Vazyme, China). Agarose gel (Biowest, France) electrophoresis was used to analyze the PCR products.
8
Colorectal Cancer Tissue Analysis
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Primary CRC tissues and paired non-tumor tissues from 37 patient volunteers were collected from the Tianjin Third Central Hospital. The clinicopathological data of these patients are provided in Table 1 . A portion of the specimens was stored at −80°C and used to investigate circ-CSPP1, miR-431, and LASP1 mRNA expression levels and analyze their correlations. Also, a portion of the specimens was used for histological analysis by two pathologists using hematoxylin and eosin (H&E) staining. Briefly, CRC samples were fixed in formaldehyde (Solarbio, Beijing, China), blocked in paraffin, and then sectioned at 4 µm intervals. The sections were stained with hematoxylin (Solarbio) for 5 min and eosin (Solarbio) for 3 min, followed by the observation of the images under a microscope (Leica, Wetzlar, Germany).
9
ChIP Assay for Histone Modifications
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The ChIP assay was performed as previously described (29 (link)). Briefly, cells were crosslinked by 1% formaldehyde (Sigma-Aldrich) in PBS for 10 min at 25°C. formaldehyde was quenched by the addition of glycine (Beijing Solarbio Science & Technology Co., Ltd.) to a final concentration of 125 µM. Then, 1×106 cells were collected by centrifugation at 300 × g for 3 min at 25°C and washed with pre-cooled PBS twice. The immunoprecipitation of crosslinked 100 µg DNA (using a spectrophotometer at 260 nm)/protein was performed using 2 µg anti-H3R2me2a (1 µg/µl, H3R2me2a; cat. no. A3155; Abclonal Biotech Co., Ltd.), anti-histone H3 lysine 4 trimethylation (1 µg/µl, H3K4me3; cat. no. A2357; Abclonal Biotech Co., Ltd.) or anti-mouse/rabbit IgG (1 µg/µl, cat. no. A7028 and A7016; Beyotime Institute of Biotechnology) antibodies for a 2-h incubation at 4°C. The immunoprecipitated DNA was purified using a ChIP DNA purification kit (cat. no. D0033; Beyotime Institute of Biotechnology) and amplified by qPCR as described above. The chip primers for the detection of H3R2me2a/H4K4me3 enrichment on p18 promotor as follows: Forward, 5′-GTCTTAAATAACAAACCCCTGTC-3′ and reverse, 5′-CTCCTCCCGTCAAGTCTCTCGCG-3′.
10
Chromatin Immunoprecipitation Assay for SP1 Transcription Factor
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According to the instruction manual of the assay kit (#WLA106a; Wanleibio), formaldehyde (Solarbio) was added to each sample and incubated at room temperature for 10 min. Glycine was incubated with the sample, and the medium was removed and washed twice with pre-chilled PBS. The cells were scraped into a 1.5 mL centrifuge tube using a cell scraper, centrifuged to collect the precipitate, and then incubated with Buffer A and Buffer B, as well as EDTA. After terminating the digestion for two min on ice, the precipitate was collected by centrifugation. The nuclear membrane was disrupted using an ultrasound bath, and anti-SP1 antibody (#MA5-35331, 1:100, Thermo Fisher) was added to the samples, which were then incubated overnight at 4 °C on a rocking mixer. The sample was then mixed with magnetic beads, allowing the supernatant to be removed by a magnetic separator. ChIP Elution Buffer was added to the magnetic beads, which were placed at 4 °C for half an hour. After DNA purification, HTR2B expression was analyzed using qRT-PCR.
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