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Isothermal buffer 2

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Isothermal buffer II is a specialized buffer solution designed for use in isothermal DNA amplification techniques. It provides a consistent and optimized environment for maintaining the activity and stability of the enzymes involved in these reactions.

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Lab products found in correlation

Six primers, by Integrated DNA Technologies (2 mentions) Sodium citrate, by Innovative Research (2 mentions) Bst3.0 polymerase, by New England Biolabs (2 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions) Evagreen, by Avantor (2 mentions) Deoxynucleotide triphosphates dntps, by Agilent Technologies (2 mentions) Diethyl pyrocarbonate depc water, by Thermo Fisher Scientific (2 mentions) Hiv 1, by American Type Culture Collection (2 mentions) Accuspan plasma, by LGC (2 mentions) Accuspan linearity panel, by LGC (2 mentions) Sphi and psti restriction enzymes, by New England Biolabs (2 mentions) Betaine, by Merck Group (2 mentions) Chikv s 27, by BEI Resources (2 mentions)

2 protocols using isothermal buffer 2

1

RT-LAMP Assay for Viral Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents necessary for the RT-LAMP assay included six primers (Integrated DNA Technologies, Skokie, IL), Bst 3.0 polymerase (NEB, Ipswich, MA), deoxynucleotide triphosphates (dNTPs) (Agilent Technologies, Santa Clara, CA), isothermal buffer II (NEB, Ipswich, MA), betaine (Millipore Sigma, Burlington, MA), EvaGreen (VWR International, Radnor, PA), ROX (Thermo Fisher Scientific, Waltham, MA), diethyl pyrocarbonate (DEPC) water (Invitrogen, Carlsbad, CA), and human whole blood collected in sodium citrate (Innovative Research, Novi, MI).
Template used in the experiments below included purified genomic RNA from HIV-1 (ATCC, Manassas, VA), non-infectious HIV-1 virus diluted in AccuSpan plasma (AccuSpan Linearity Panel, SeraCare Life Sciences, Milford, MA), purified genomic RNA from dengue virus (DENV) type 1 (BEI resources, Manassas, VA), and purified RNA from chikungunya virus (CHIKV) S-27 (BEI resources, Manassas, VA). SphI and PstI restriction enzymes (NEB, Ipswich, MA) and phosphate buffered saline (PBS) (Thermo Fisher Scientific, Waltham, MA) are additional reagents used.
Phillips E.A., Moehling T.J., Ejendal K.F., Hoilett O.S., Byers K.M., Basing L.A., Jankowski L.A., Bennett J.B., Lin L.K., Stanciu L.A, & Linnes J.C. (2019). Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples. Lab on a chip, 19(20), 3375-3386.
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2

RT-LAMP Assay for Viral Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents necessary for the RT-LAMP assay included six primers (Integrated DNA Technologies, Skokie, IL), Bst 3.0 polymerase (NEB, Ipswich, MA), deoxynucleotide triphosphates (dNTPs) (Agilent Technologies, Santa Clara, CA), isothermal buffer II (NEB, Ipswich, MA), betaine (Millipore Sigma, Burlington, MA), EvaGreen (VWR International, Radnor, PA), ROX (Thermo Fisher Scientific, Waltham, MA), diethyl pyrocarbonate (DEPC) water (Invitrogen, Carlsbad, CA), and human whole blood collected in sodium citrate (Innovative Research, Novi, MI).
Template used in the experiments below included purified genomic RNA from HIV-1 (ATCC, Manassas, VA), non-infectious HIV-1 virus diluted in AccuSpan plasma (AccuSpan Linearity Panel, SeraCare Life Sciences, Milford, MA), purified genomic RNA from dengue virus (DENV) type 1 (BEI resources, Manassas, VA), and purified RNA from chikungunya virus (CHIKV) S-27 (BEI resources, Manassas, VA). SphI and PstI restriction enzymes (NEB, Ipswich, MA) and phosphate buffered saline (PBS) (Thermo Fisher Scientific, Waltham, MA) are additional reagents used.
Phillips E.A., Moehling T.J., Ejendal K.F., Hoilett O.S., Byers K.M., Basing L.A., Jankowski L.A., Bennett J.B., Lin L.K., Stanciu L.A, & Linnes J.C. (2019). Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples. Lab on a chip, 19(20), 3375-3386.
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