Hp 5ms agilent column

All chemicals were purchased from commercial
suppliers and used directly as received, unless noted otherwise. The
plasmid pACYC-GroEL/ES-TF was a gift from Karl Griswold (Addgene plasmid
#83923; http://n2t.net/addgene:83923; RRID: Addgene 83923). Metal analysis was conducted in the Microanalytical
Facility in the College of Chemistry at the University of California,
Berkeley, by using a PerkinElmer ICP Optima 7000 DV spectrometer.
All degassed chemicals were rendered anaerobic by equilibration overnight
in a Coy anaerobic chamber equipped with a 1.5–2.5% H₂/N₂ environment and a palladium catalyst. All aqueous
solutions were prepared using water purified by a Milli-Q Academic
water purifier and exhibited a conductivity of 18.2 MΩ. Genetic
sequencing was performed at the University of California, Berkeley,
DNA Sequencing Facility. Large-scale (>0.5 L) bacterial cultures
were
grown using a LEX-48 bioreactor (Epiphyte3). An Agilent 7890 GC-MS
system equipped with an HP-5MS Agilent column (30m × 0.250 mm
× 0.25 μm) at the Lawrence Berkeley National Laboratory
Catalysis Laboratory was used for GC-MS analysis. Nuclear magnetic
resonance (NMR) spectra were collected on a Bruker AVQ-400 spectrometer
at the University of California, Berkeley, College of Chemistry NMR
Facility.

Relative abundances of essential oil components and esterified diterpene acids were studied using gas chromatography with mass spectrometric detection (GC-MS). GC-MS analyses were performed using an Agilent Technologies 7890A GC-System coupled with an Agilent 5975C mass selective detector (triple-Axis detector, Agilent Technologies, Wilmington, DE, USA). An autosampler unit (Agilent Technologies 7693-100 positions) held samples. Separation of 1-μL injections used an HP-5MS Agilent column (30 m × 250 μm × 0.25 μm). Operating conditions were as follows: injector split ratio 25:1, temperature 250 °C, carrier gas helium, 1.0 mL/min, and constant flow. Column temperature was 50 °C (no hold) and 5 °C per minute. Then, at 280 °C, it was held at 5 min. Mass fragmentation patterns were acquired at −70 eV using a mass scan range of m/z 30–400.
Primary identifications were performed by comparison of mass spectra with an electronic library database [54 ] and confirmed using arithmetic indices, calculated relative to n-alkanes, when compared with values published in Adams [55 ] by visual comparison against mass spectral images [55 ]. Semi-quantification was achieved by the GC-MS operating software, using data with a minimum peak area of 0.1%, by calculating the area under the curve.
HRESIMS spectra were recorded using an AB Sciex 5600 TripleTOF mass spectrometer in positive mode.

Gas chromatography coupled mass spectrometry analyses of TMS-derivatized culture extracts were performed using an HP 6890 series GC system fitted with an HP 5973 mass-selective detector and a 30 m × 250 μm HP-5MS Agilent column. The operating conditions were: T_GC (injector), 280°C; T_MS (ion source), 230°C; oven time program T_{0 min}, 104°C, T_{2 min}, 104°C, T_{14.4 min}, 290°C (heating rate 15°C·min^-1), T_{29.4 min}; 290°C.