Immunoblot analysis was carried out under standard conditions (Marrocco et al., 2012 (link)) using the following primary antibodies: anti-tyrosine hydroxylase (TH H126, Santa Cruz, 1:1000); anti-D1 receptors (Ab20066, Abcam, 1:500), anti-D2 receptors (Ab85367, Abcam, 1:500), anti-DAT (high affinity DA transporter) (ab111468, Abcam, 1:1000); anti-adenosine receptors (sc-13937, Santa Cruz, 1:500), synaptic vesicle-associated proteins: anti-Rab3a (#107111, Synaptic Systems, 1:2000); anti-Munc18 (#116011, Synaptic Systems 1:2000; anti-SNAP 25 (sc-136,267, Santa Cruz 1:5000); anti-SYP (sc-9116 Santa Cruz 1:8000), and anti-syntaxin (sc-13994, Santa Cruz 1:4000). Secondary antibodies directed against rabbit or mouse antibodies (Amersham) were used at 1:7500 dilution. After immunoblotting, digitized images of bands immunoreactive to the target antibodies and actin were acquired (FUSION®) and the area of immunoreactivity corresponding to each band was measured using ImageJ imaging software. The ratios of the targets to actin were then determined and these values were compared to check statistical significance.
Anti munc18
Manufactured by Synaptic Systems
Sourced in Germany
Anti-Munc18 is a laboratory research tool used to study the role of the Munc18 protein in various cellular processes. Munc18 is a key regulator of membrane fusion events, and Anti-Munc18 can be used to investigate its functions.
Automatically generated - may contain errors
Lab products found in correlation
Ab20066, by Abcam (1 mentions)
Ab85367, by Abcam (1 mentions)
Ab111468, by Abcam (1 mentions)
Anti snap25, by Santa Cruz Biotechnology (1 mentions)
Anti syp, by Santa Cruz Biotechnology (1 mentions)
Sc 13937, by Santa Cruz Biotechnology (1 mentions)
Anti syntaxin, by Santa Cruz Biotechnology (1 mentions)
Anti vamp2, by Synaptic Systems (1 mentions)
Anti snap25, by Abcam (1 mentions)
Ab193349, by Abcam (1 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
β actin, by Merck Group (1 mentions)
2 protocols using anti munc18
1
Western Blot Analysis of Neurotransmitter Receptors
2
Western Blot Analysis of Synaptic Proteins
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Total protein samples for western blot analysis were extracted from cultured NRNs or the left ventricle and hippocampi of the mice after they were anaesthetized with sodium pentobarbital (100 mg/kg, i.p.). Mouse death was then confirmed by exsanguination according to a previously described method [31 (link)]. Hippocampi for primary cell culture were collected from neonatal Sprague-Dawley (SD) rats after the administration of 20% isoflurane and confirmation of death by cervical dislocation. Anti-SNAP-25 (1:1000, ab5666, Abcam, MA, USA), anti-VAMP-2 (1:10000, 104,211, Synaptic Systems, Gottingen, Germany) and anti-Syntaxin-1A (1:5000, 100,111, Synaptic Systems, Gottingen, Germany), anti-Munc-18 (1:1000, 116,002, Synaptic Systems, Gottingen, Germany), anti-CD63 (1:1000, ab193349, Abcam, MA, USA),were used as primary antibodies. β-actin (1:1000, G8795, Sigma, Saint Louis, MO, USA) was selected as an internal control. The blots were detected with an Odyssey Infrared Imaging System (LI-COR Biosciences, Lincoln, NE, USA). The final results were expressed as fold changes compared with the control values.
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