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Non oxidized oligosaccharides

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Non-oxidized oligosaccharides are a type of laboratory equipment used for the analysis and characterization of carbohydrate molecules. These compounds are composed of multiple monosaccharide units linked together, maintaining their original chemical structure without oxidation. The core function of non-oxidized oligosaccharides is to serve as standards or reference materials in analytical procedures for the identification and quantification of similar carbohydrate structures.

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Lab products found in correlation

Carbopac pa1 column, by Thermo Fisher Scientific (1 mentions) Carbopac pa 1 guard column, by Thermo Fisher Scientific (1 mentions) Hpaec pad, by Thermo Fisher Scientific (1 mentions)

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Oligosaccharides

2 protocols using non oxidized oligosaccharides

1

Quantitative Analysis of Saccharides

Cited 1 time
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Mono-, oligo-saccharides and their corresponding aldonic acids present in S2 were analysed by ionic chromatography (HPAEC) as already described [37 (link)], using non-oxidized oligosaccharides (Megazyme, Bray, Ireland) as standards. Gluconic acid (Megazyme) and corresponding C1-oxidized standards (from DP2 to DP6) were produced from non-oxidized cello-oligosaccharides using a cellobiose dehydrogenase treatment [37 (link)].
Paës G., Navarro D., Benoit Y., Blanquet S., Chabbert B., Chaussepied B., Coutinho P.M., Durand S., Grigoriev I.V., Haon M., Heux L., Launay C., Margeot A., Nishiyama Y., Raouche S., Rosso M.N., Bonnin E, & Berrin J.G. (2019). Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance. Biotechnology for Biofuels, 12, 76.
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2

Cellooligosaccharides Analysis by HPAEC-PAD

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Oxidized and non-oxidized cellooligosaccharides generated after LPMO action were analyzed by high-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) (ThermoFischer Scientific, IL) using a CarboPac™ PA1 column (2 × 250 mm) and CarboPac™ PA1 guard column (2 × 50 mm) at a 0.25 mL min−1 flow rate as in [49 (link)]. Non-oxidized oligosaccharides were used as standards (Megazyme, Wicklow, Ireland).
Chalak A., Villares A., Moreau C., Haon M., Grisel S., d’Orlando A., Herpoël-Gimbert I., Labourel A., Cathala B, & Berrin J.G. (2019). Influence of the carbohydrate-binding module on the activity of a fungal AA9 lytic polysaccharide monooxygenase on cellulosic substrates. Biotechnology for Biofuels, 12, 206.
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