Hdac1 antibody

Manufactured by Cell Signaling Technology

Sourced in United States

The HDAC1 antibody is a tool used by researchers to detect and study the HDAC1 protein, which is a histone deacetylase enzyme involved in the regulation of gene expression. The antibody can be used in various laboratory techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to identify and quantify the HDAC1 protein in biological samples.

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Lab products found in correlation

Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions) Bca assay, by Thermo Fisher Scientific (1 mentions) Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions) Image studio lite, by LI COR (1 mentions) Sin3a antibody, by Abcam (1 mentions) Hdac2, by Cell Signaling Technology (1 mentions) Dynabeads protein g, by Thermo Fisher Scientific (1 mentions) Trans blot turbo transfer system, by Bio-Rad (1 mentions) Agarose, by Merck Group (1 mentions) Taq polymerase, by Takara Bio (1 mentions) Protein g agarose, by Merck Group (1 mentions) Peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) Ecltm western blotting detection reagent, by Cytiva (1 mentions) Dnase ι, by Roche (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions) Pipes, by Merck Group (1 mentions) Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (1 mentions) Acetyl histone h3, by Cell Signaling Technology (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Tween 20, by Merck Group (1 mentions)

4 protocols using hdac1 antibody

Assessing HDAC-Sin3a Interaction in Heart

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Protein extracts were prepared in RIPA lysis buffer (Thermo Fisher Scientifics) from snap-frozen heart tissues or NRCMs. Protein concentration was measured by BCA assay (Thermo Fisher Scientifics). The protein electrophoresis were performed on 4–12% Bis-Tris NuPage gels (Thermo Fisher Scientifics). The Bio-Rad Trans-Blot Turbo Transfer System was used for the proteins transfer to nitrocellulose membranes. Secondary antibodies used were from LI-COR Biosciences. The blots were quantified using Image J or LI-COR Image studio Lite. HDAC1, HDAC2 and HDAC3 antibodies were from Cell Signaling Technologies (Cat#5356, #5113 and #3949 respectively). The Sin3a antibody was from Abcam, # ab129087.
Co-immunoprecipitation: HDAC1 was immunoprecipitated from 500μg heart lysates (in RIPA buffer) using 0.5μg HDAC1 antibody (Cell Signaling Technologies Cat#5356), and 25 μl Dynabeads™ Protein G (Thermo Fisher Scientifics). Co-immunoprecipitated HDAC2 and Sin3a were probed using antibody from Cell Signaling Technologies (#5113) and Abcam (# ab129087) respectively.

Zhang M., Yang X., Zimmerman R.J., Wang Q., Ross M.A., Granger J.M., Luczak E.D., Bedja D., Jiang H, & Feng N. (2020). CaMKII exacerbates heart failure progression by activating class I HDACs. Journal of molecular and cellular cardiology, 149, 73-81.

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Molecular Investigations in Neuroscience

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Agarose, PIPES, Potassium hydroxide, Tween^® 20, 2-mercaptoethanol, Trypsin, MPH, ATX and VPA were purchased from Sigma (St. Louis, MO, USA). ECL^TM Western blotting detection reagents were obtained from Amersham Life Science (Arlington Heights, IL, USA). Trizol and SuperScript^TM II Reverse Transcriptase were purchased from Invitrogen (Carlsbad, CA, USA). DNase Ι was purchased from Roche (Mannheim, Germany). Protein G Agarose was obtained from Millipore Corporation (Billerica, MA, USA). Taq polymerase and dNTP were obtained from Takara (Shiga, Japan). Protease inhibitor cocktail was obtained from Calbiochem (La Jolla, CA, USA). Chelex 100 was obtained from BioRad (Hercules, CA, USA). Antibodies were purchased from the following companies: anti-β-actin from Sigma (St. Louis, MO, USA), Histone H3, Acetyl-Histone H3 and HDAC1 antibody from Cell signaling (Boston, MA, USA), DAT, NET and peroxidase-conjugated secondary antibody from Santa Cruz biotechnology (Dallas, TX, USA).

Choi C.S., Hong M., Kim K.C., Kim J.W., Yang S.M., Seung H., Ko M.J., Choi D.H., You J.S., Shin C.Y, & Bahn G.H. (2014). Effects of Atomoxetine on Hyper-Locomotive Activity of the Prenatally Valproate-Exposed Rat Offspring. Biomolecules & Therapeutics, 22(5), 406-413.

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Protein Isolation and Western Blot

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Total protein was isolated with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. Protein concentrations were measured using a BCA protein kit (Thermo Fisher Scientific, Rockford, IL), and proteins were separated on SDS-polyacrylamide gels and transferred onto PVDF membranes (Immobilon-P; Millipore, Bedford, MA, USA). The membranes were blocked with 5% nonfat dried milk in TBST (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, and 0.1% Tween-20) for 1 h at RT and then blotted overnight with HDAC1 antibody (1:1000 dilution, Cell Signaling Technology Inc., Danvers, MA, USA) or anti-β-actin antibody (1:1000 dilution, Sigma) at 4 °C.

He Y., Tang D., Li W., Chai R, & Li H. (2016). Histone deacetylase 1 is required for the development of the zebrafish inner ear. Scientific Reports, 6, 16535.

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Comprehensive Immunoblotting Analysis of Key Signaling Proteins

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All immunoblotting analyses were performed following SDS PAGE using standard methods. Antibodies used for immunoblotting were HIF1α antibody (Abcam, CAT# ab51608, dilution 1:1000), HIF1α antibody (Cell Signaling Technology, CAT# 79233, dilution 1:1000), EZH2 antibody (Cell Signaling Technology, CAT# 5246, dilution 1:1000), SUZ12 antibody (Cell Signaling Technology, CAT# 3737, dilution 1:1000), EED antibody (Millipore, CAT# 09-744, dilution 1:1000), HDAC1 antibody (Cell Signaling Technology, CAT# 34589, dilution 1:1000), HDAC2 antibody (Cell Signaling Technology, CAT# 57156, dilution 1:1000), phosphorylated-STAT1 antibody (Cell Signaling Technology, CAT# 9167, dilution 1:1000), STAT1 antibody (Cell Signaling Technology, CAT# 9176, dilution 1:1000), PD-L1 antibody (Cell Signaling Technology, CAT# 3684, dilution 1:1000), IRF1 antibody (Abcam, CAT# ab26109, dilution 1:1000), H3K27me3 antibody (Cell Signaling Technology, CAT# 9733, dilution 1:1000), H3K27ac antibody (Abcam, CAT# 4729, dilution 1:1000), and GAPDH antibody (Cell Signaling Technology, CAT# 2118, dilution 1:1000).

Ma S., Zhao Y., Lee W.C., Ong L.T., Lee P.L., Jiang Z., Oguz G., Niu Z., Liu M., Goh J.Y., Wang W., Bustos M.A., Ehmsen S., Ramasamy A., Hoon D.S., Ditzel H.J., Tan E.Y., Chen Q, & Yu Q. (2022). Hypoxia induces HIF1α-dependent epigenetic vulnerability in triple negative breast cancer to confer immune effector dysfunction and resistance to anti-PD-1 immunotherapy. Nature Communications, 13, 4118.

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