Anti-OTUD1 (HPA038504, 1:1000) and Anti-OTUD1 (HPA038503, 1:200) were purchased from Atlas Antibodies. Anti-OTUD1 (29921-1-AP, 1:200), anti-ASK1 (67072-1-Ig, 1:1000), Anti-ERK (11257-1-AP, 1:1000), anti-p38 (114064-1-AP, 1:1000), anti-JNK, (66210-1-Ig, 1:1000), anti-HSP70, (10995-1-AP, 1:200), anti-P62 SQSTM1, (18420-1-AP, 1:200), anti-GAPDH, (60004-1-Ig, 1:3000), and anti-Flag tag, (20543-1-AP, 1:1000) were purchased from Proteintech. Anti-phospho-p44/42 MAPK (Erk1/2) Thr202/Tyr204) (#9101, 1:1000), anti-phospho- p38 MAPK (Thr180/Tyr182) (#9211, 1:1000), anti-SAPK/JNK (Phospho-Thr183/Tyr185) (81E11) (#4668, 1:1000), anti-c-Jun (Phospho-Ser73) (D47G9), (#3270, 1:1000), and anti-c-Jun (#9165, 1:1000) were purchased from Cell Signaling Technology. Anti-ASK1 (380952, 1:1000) was purchased from. Anti-ASK1 (Phospho-Thr838) (orb335764, 1:1000) was purchased from Biorbyt. anti-HA tag (2063, 1:1000) and anti-Myc tag (2097, 1:1000) were purchased from DiaAn Biotech. Goat anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab15007, 1:200), Goat anti-Rabbit IgG H&L (Alexa Fluor 555) (ab150078, 1:200), APC Mouse Anti-Human CD44(G44-26) (559942, 1:50), and FITC Mouse Anti-Human CD133(W6B3C1) (567029, 1:50) were purchased from BD Pharmingen. Goat anti- Rabbit IgG H&L (HRP) (BF03008, 1:5000) and Goat anti-Mouse IgG H&L (HRP) (BF03001, 1:5000) were purchased from Biodragon,
Anti jnk
Manufactured by Proteintech
Sourced in China, United States
Anti-JNK is a laboratory reagent used for the detection and quantification of the c-Jun N-terminal kinase (JNK) protein. JNK is a key signaling molecule involved in various cellular processes, including cell growth, differentiation, and stress response. The Anti-JNK product is a specific antibody that can be used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to identify and measure the levels of JNK in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti p38, by Proteintech (7 mentions)
Anti p jnk, by Proteintech (5 mentions)
Anti erk1 2, by Proteintech (5 mentions)
Anti gapdh, by Proteintech (4 mentions)
Anti caspase 3, by Proteintech (3 mentions)
Anti bcl 2, by Proteintech (3 mentions)
Anti β actin, by Proteintech (3 mentions)
Anti akt, by Proteintech (3 mentions)
Anti phospho jnk, by Proteintech (2 mentions)
Anti p p38, by Proteintech (2 mentions)
Anti smad2, by Cell Signaling Technology (2 mentions)
Anti lc3, by Proteintech (2 mentions)
Anti phosphorylation p38, by Beyotime (2 mentions)
Anti phosphorylation erk1 2, by Beyotime (2 mentions)
Anti phosphorylation jnk, by Beyotime (2 mentions)
Protease inhibitor, by Beyotime (2 mentions)
Bca protein assay kit, by Beyotime (2 mentions)
Anti p62, by Proteintech (2 mentions)
Anti bax, by Proteintech (2 mentions)
Anti phospho p38 mapk thr180 tyr182, by Cell Signaling Technology (1 mentions)
20 protocols using anti jnk
1
Antibody Validation for Protein Signaling
2
Western Blot Analysis of SCI in Rats
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28 days following SCI, euthanized rats under isoflurane anesthesia. Samples were then collected and fixed in 4% paraformaldehyde. A 0.5 cm long sample tissue was homogenized in 300 μL of lysate-containing protease inhibitors. Following lysate preparation, the electrophoresed tissue lysate was transferred to polyvinylidene difluoride membranes. Next, the membranes were blocked with 5% skimmed milk and incubated in an incubator for 60 min. The membranes were further blocked for two hours and then incubated with primary antibodies, consisting of anti-JNK (1:1000; Proteintech), anti-p-JNK (1:1000; Proteintech), anti-p-p38 (1:500; Bioss), anti-p38 (1:1000; Bioss), and GAPDH (1:2000, UtiBody). They were then incubated with secondary antibodies (1:3000; Bioss) in an incubator for 1 h. ImageJ was used to quantify grey bands.
3
Protein Expression Analysis in Oocyte Development
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Total protein was extracted from GCs, oocytes, and ovaries using RIPA buffer (Solarbio, R0020, China). Protein concentration was assessed by BCA assay (Cellchip, China). Protein extracts separated by SDS-PAGE were blotted with the following primary antibodies: anti-IRE1α (1:1000, CST, 3294), anti-phospho-IRE1 (Ser724, 1:1000, Abcam, ab48187), anti-Bcl2 (1:500, Proteintech, 12789-1-AP), anti-Bax (1:500, Ruiying, RLT0456), anti-JNK (1:500, Proteintech, 51151-1-AP), anti-phospho-JNK (Thr183/Tyr185, 1:1000, CST, 4688), anti-caspase 9 (1:1000, CST, 9508), anti-cleaved caspase 9 (1:1000, CST, 9509) anti-caspase 3, (1:700, Proteintech, 19677-1-AP), anti-cleaved caspase 3 (1:1000, CST, 9661), anti-XBP1 (1:400, Santa Cruz, sc8015), anti-ATF6 (1:400, Santa Cruz, sc166659), anti-BiP/GRP78 (1:1000, Santa Cruz, sc376768), anti-phospho-PERK (Thr980, 1:1000, CST, 3179), anti-PERK (1:1000, CST, 3192), anti-phospho-eIF2α (Ser51, 1:1000, CST, 3398), anti-eIF2α (1:1000, CST, 9722), anti-ATF4 (1:1000, CST, 11815), anti-CHOP (1:500, Proteintech, 15204-1-AP), and TMCO1 antibody (1:1000) [13 (link)].
4
Protein Expression Analysis of Pulmonary Artery Cells
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The PAs homogenate tissue and collected HPASMCs and HPAECs were dissolved in proteolytic buffer for 30 minutes. After 30 minutes, the lysed PAs homogenate tissue and cell proteins were centrifuged at 13 500 g for 15 minutes, and then the protein concentration was measured using a BCA kit. Thirty micrograms of cell lysate from each sample were used for SDS‐PAGE (Bio‐Rad Laboratories), and Western blotting were analysed according to the protocol as described previously.23 The chosen antibodies included anti‐NDUFA4L2 (Catalogue number: 16480‐1‐AP; ProteinTech), anti‐HIF1α (Catalogue number: AF1009; Affinity), anti‐PCNA (Catalogue number: 10205‐2‐AP; ProteinTech), anti‐cyclin A (Catalogue number: 13295‐1‐AP; ProteinTech), anti‐cyclin E (Catalogue number: 11554‐1‐AP; ProteinTech), anti‐ERK (Catalogue number: 16443‐1‐AP; ProteinTech), anti‐p‐ERK (Catalogue number: AF1015; Affinity), anti‐5‐LO (Catalogue number: AF4699; Affinity), anti‐p‐5‐LO (Catalogue number: AF8359; Affinity), anti‐p38 (Catalogue number: 14064‐1‐AP; ProteinTech), anti‐p‐p38 (Catalogue number: 4511T; Cell Signaling), anti‐JNK (Catalogue number: 51151‐1‐AP; ProteinTech) and anti‐p‐JNK (Catalogue number: 4668S; Cell Signaling) et al. After extensive washes membranes with TBS‐T, the ECL luminescent solution is added for exposure and development.
5
Autophagy Regulation by Signaling Pathways
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These following reagents were used in our research: crystal violet (Beyotime, #C0121), MTT (Solarbio, #M8180), dimethyl sulfoxide (DMSO, Sangon biotech, #A503039), a BCA protein assay kit (Solarbio, #PC0020), chloroquine (CQ, Sigma-Aldrich, #C6628), LysoTracker Red DND-99 (Invitrogen, #L7528), fetal bovine serum (FBS, Biological Industries, #04-001-1A), trypsin-EDTA Solution (BBI, #E607001), Earle’s balanced salts solution (EBSS, Gibco, #24010043), BeyoECL Plus (Beyotime, #P0018S), and Lipofectamine 2000 reagent (Invitrogen, #11668027).
Antibodies were obtained as follows: anti-LC3 antibody (Sigma-Aldrich, L7543), anti-α-tubulin (Sigma-Aldrich, T6199), anti-FLAG (Sigma-Aldrich, F3165), anti-β-actin (Sigma-Aldrich, A5441), anti-phospho-JNK (Proteintech, 80024) and anti-JNK (Proteintech, 66210). All of the other antibodies were purchased from Cell Signaling Technology: anti-phospho-AKT (cata. no. 4060), anti-AKT (cata. no. 4691), phospho-mTOR (cata. no.5536), anti-mTOR (cata. no. 2983), anti-phospho-S6 (cata.no. 2211), anti-S6 (cata. no. 2217), anti-phospho-4E-BP1 (cata.no. 2855), anti-4E-BP1(cata.no. 9452), anti-ERK1/2 (cata.no. 9102), anti-phospho-ERK1/2 (cata.no. 9101), anti-TSC2 (cata.no. 4308), anti-phospho-p38 (cata.no. 4511) and anti-p38 (cata.no 8690).
Antibodies were obtained as follows: anti-LC3 antibody (Sigma-Aldrich, L7543), anti-α-tubulin (Sigma-Aldrich, T6199), anti-FLAG (Sigma-Aldrich, F3165), anti-β-actin (Sigma-Aldrich, A5441), anti-phospho-JNK (Proteintech, 80024) and anti-JNK (Proteintech, 66210). All of the other antibodies were purchased from Cell Signaling Technology: anti-phospho-AKT (cata. no. 4060), anti-AKT (cata. no. 4691), phospho-mTOR (cata. no.5536), anti-mTOR (cata. no. 2983), anti-phospho-S6 (cata.no. 2211), anti-S6 (cata. no. 2217), anti-phospho-4E-BP1 (cata.no. 2855), anti-4E-BP1(cata.no. 9452), anti-ERK1/2 (cata.no. 9102), anti-phospho-ERK1/2 (cata.no. 9101), anti-TSC2 (cata.no. 4308), anti-phospho-p38 (cata.no. 4511) and anti-p38 (cata.no 8690).
6
Comprehensive Western Blot Methodology
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Western blot was performed as previously described20 (link). Antibodies used in this study were PDLIM1 (Affinity, DF3003), α-SMA (Affinity, AF1032), COL1A1 (Cell Signaling Technology, #91144), CTCF (Millipore, 07-729), TNF-α (Affinity, AF7014), IL-6 (Affinity, DF6087), p65(Affinity, AF5006), anti-p-Smad2/3 (Abcam, ab254407), anti-Smad2/3 (Abcam, ab202445), anti-p-ERK (Abcam, ab201015), anti-ERK (Abcam, ab184699), anti-p-P38 (Proteintech, 28796-1-AP), anti-P38 (Proteintech, 66234-1-Ig), anti-p-JNK (Proteintech, 80024-1-RR), anti-JNK (Proteintech, 66210-1-Ig), GAPDH (Cell Signaling Technology, #2118). Western blot analysis was performed on at least three independent biological replicates.
7
Fibrosis Signaling Pathway Modulation
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BLM and the JNK inhibitor (SP600125) were obtained from Selleck China Inc. (Shanghai, China). TGF-β1 was purchased from PeproTech China Inc. (Suzhou, China). Azithromycin was obtained from Sigma-Aldrich Inc. (Shanghai, China). The primary antibodies we used are as follows: anti-vimentin (Proteintech, 60330-1-Ig), anti-alpha-smooth muscle actin (α-SMA) (Proteintech, 14395-1-AP), anti-Collagen 1 (Proteintech, 14695-1-AP), anti-LOX (Proteintech, 17958-1-AP), anti-LOXL2 (Abcam, 96233), anti-TGF-β1 (Proteintech, 21898-1-AP), anti-Smad2 (Cell Signaling Technology, 5339), anti-Smad3 (Cell Signaling Technology, 9523), anti-phospho (P)-smad2 (Cell Signaling Technology, 3108), anti-P-smad3 (Cell Signaling Technology, 9520), anti-JNK (Proteintech, 66210-1-Ig), anti-c-Jun (Proteintech, 66313-1-Ig), anti-P-JNK (Proteintech, 80024-1-RR), anti-P-cJun (Proteintech, 28891-1-AP), anti-α-tubulin (Proteintech, 66031-1-Ig), and anti-GAPDH (Proteintech, 60004-1-Ig). The dilution ratio of all antibodies was 1:1000.
8
Western Blot Analysis of Neuroinflammatory Markers
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Total protein from PC12 cells or the hippocampal tissues was extracted and analyzed using Western blots. Prepared samples were separated by 10% or 12% SDS-PAGE and transferred to Polyvinylidene Fluoride (PVDF) membranes (Millpore, Bedford, MA, USA). The membranes were incubated with anti-GAPDH (Abcam, Cambridge, UK), anti-thr181-phosphorylated-tau, anti-thr205-phosphorylated-tau, anti-ser396-phosphorylated-tau, anti-total tau, anti-JNK, anti-phospho JNK, anti-ERK1/2, anti-phospho ERK1/2, anti-phospho p38, anti-p38, anti-NF-κB, anti-phospho NF-κB (CST), anti-IL-6, and anti-TNF-α (Proteintech, Wuhan, China) antibodies. Immunoreactive bands were detected with the appropriate horseradish peroxidase-conjugated secondary antibodies and immunological complexes were visualized by enhanced chemiluminescence reagents (Pierce, Rockford, IL, USA).
9
Western Blot Analysis of Cellular Signaling
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Total protein separation and western blotting were performed as described previously [16 (link)]. Western blot analysis was performed using anti-cleaved PARP (#5625), anti-cleaved caspase-3 (#9661), anti-p-AMPK (Thr172) (#50081), anti-p-ERK1/2 (#4370), and anti-HA-Tag (#3724) antibodies purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-LC3 (14600–1-AP), anti-P62/SQSTM1 (18420–1-AP), anti-phospho-Akt (Ser-473) (66444–1-Ig), anti-Akt (10176–2-AP), anti-mTOR (20657–1-AP), anti-P38 MAPK (14064–1-AP), anti-JNK (51151–1-AP), anti-ERK1/2 (16443–1-AP), anti-AMPK (10929–2-AP), anti-Nrf2 (16396–1-AP), anti-Keap1 (10503–2-AP), anti-FLAG-tag (66008–2-AP), anti-MYC-tag (60003–2-AP), and anti-β-actin (66009–1-Ig) antibodies were obtained from Proteintech Group Co., Ltd. (Wuhan, China). An anti-phospho-mTOR (Ser-2448) (ab109268) antibody was purchased from Abcam (Cambridge, MA, USA). Anti-p-p38 (sc-7973), anti-p-JNK (sc-6254), and anti-Ub (sc-8017) antibodies were obtained from Santa Cruz Biotechnology., Inc. (Santa Cruz, CA, USA).
10
Analysis of Adipose Tissue MAPK Signaling
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Mouse adipose tissue was homogenized with a tissue homogenizer at centrifuge of 12000 g at 4 °C for 10 min using RIPA lysis buffer containing protease inhibitor (Beyotime Biotechnology) and phosphatase inhibitor (MedChemExpress).The supernatant was aspirated, and the protein concentration was measured by the BCA Protein Assay Kit (Vazyme Biotech Co., Ltd.).The protein sample was loaded for polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane in an electrophoresis transfer buffer containing 10% methanol. The membrane was blocked with 5% BSA (Sigma-Aldrich) for 2 h at room temperature, followed by incubation overnight at 4 °C with anti-p38(1:500; Proteintech™), anti-phosphorylation p38(1:1000;Beyotime), anti-ERK1/2(1:2000; Proteintech), anti-phosphorylation ERK1/2(1:1000; Beyotime), anti-JNK(1:3000; Proteintech™), anti-phosphorylation JNK (1:1000; Beyotime), and anti-GAPDH (1:50,000; Proteintech Wuhan, China) antibodies. After overnight incubation, the blots were incubated with the corresponding secondary antibodies (1:10,000; Bioworld) for 1 h at room temperature. Bands of target proteins were visualized using an enhanced chemiluminescence kit (Vazyme Biotech Co.,Ltd).
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