Open field chamber

Manufactured by Harvard Apparatus

Sourced in United States

The Open-field chamber is a behavioral testing apparatus designed for the observation and assessment of rodent locomotor activity and exploration in an open environment. It provides a standardized setup for researchers to study spontaneous activity, anxiety-like behaviors, and other exploratory behaviors in a controlled setting.

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Lab products found in correlation

Truscan software, by Harvard Apparatus (4 mentions) D amphetamine sulfate, by Merck Group (4 mentions) Tru scan activity system, by Harvard Apparatus (2 mentions) Truscan photo beam activity system, by Harvard Apparatus (1 mentions)

7 protocols using open field chamber

Locomotor Activity in Stressed Rats

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Between 1 and 2 or 5 and 6 weeks after the stress, rats were tested in an open-field chamber (Coulbourn Instruments) in which locomotor activity was determined by beam breaks and recorded with TruScan software (Coulbourn Instruments). Basal locomotor activity was recorded for 30 min. After that, rats were injected with saline (1 mL/kg) or D-amphetamine sulfate (0.75 mg/kg, i.p.; Sigma) and their locomotor activity was recorded for another 90 min.

Gomes F.V., Zhu X, & Grace A.A. (2019). The pathophysiological impact of stress on the dopamine system is dependent on the state of the critical period of vulnerability. Molecular psychiatry, 25(12), 3278-3291.

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Locomotor Activity in Stressed Rats

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Open Field Locomotor Activity

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Mouse was placed in an open field chamber (Coulbourn Instruments) and allowed to explore freely for 2 hrs. The locomotor activity (such as travel distance) in the whole chamber as well as in the center area was monitored and analyzed by an automated system (the TruScan Activity System, Coulbourn Instruments).

Ding Q., Sethna F, & Wang H. (2014). Behavioral analysis of male and female Fmr1 knockout mice on C57BL/6 background. Behavioural brain research, 0, 72-78.

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Open Field Locomotor Activity Assay

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Each subject was placed in an open field chamber (Coulbourn Instruments) and allowed to explore freely for 120 min. The locomotor activity including total distance traveled (in cm), vertical activity, time spent in the center, and the beam-break counts for stereotyped behaviors in the whole chamber as well as in the center area was monitored and analyzed by an automated system (the TruScan Activity System, Coulbourn Instruments).

Huang M.W., Lin Y.J., Chang C.W., Lei F.J., Ho E.P., Liu R.S., Shyu W.C, & Hsieh C.H. (2018). RGS4 deficit in prefrontal cortex contributes to the behaviors related to schizophrenia via system xc--mediated glutamatergic dysfunction in mice. Theranostics, 8(17), 4781-4794.

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Open Field Locomotor Activity

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Immediately after the 2-h physical restraint, mice were placed in the center of an open field chamber (Coulbourn Instruments, Whitehall, PA) and allowed to explore freely for 30 min. The overall locomotive exploratory activity in the whole open field arena, as well as in the center area, was recorded and analyzed with the TruScan Photo Beam Activity System (Coulbourn Instruments).

Yang M., Ding Q., Zhang M., Moon C, & Wang H. (2020). Forebrain overexpression of type 1 adenylyl cyclase promotes molecular stability and behavioral resilience to physical stress. Neurobiology of Stress, 13, 100237.

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Amphetamine-Induced Locomotor Activity

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Adult rats were tested in an open-field chamber (Coulbourn Instruments) in which locomotor activity was determined by beam breaks and recorded with TruScan software (Coulbourn Instruments). All experiments were conducted at the same time each day. Spontaneous activity was recorded for 30min. After that, rats were injected with D-amphetamine sulfate (0.5mg/kg, i.p.; Sigma-Aldrich) and their locomotor activity was recorded for another 90min.

Gomes F.V., Guimarães F.S, & Grace A.A. (2015). Effects of Pubertal Cannabinoid Administration on Attentional Set-Shifting and Dopaminergic Hyper-Responsivity in a Developmental Disruption Model of Schizophrenia. International Journal of Neuropsychopharmacology, 18(2), pyu018.

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Locomotor Activity in Amphetamine-Treated Rats

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Adult rats were tested in an open-field chamber (Coulbourn Instruments, USA) in which locomotor activity was determined by beam breaks and recorded with TruScan software (Coulbourn Instruments). All experiments were conducted at the same time each day. Spontaneous activity was recorded for 30 min. After that, rats were injected with D-amphetamine sulfate (0.5 mg/kg, i.p.; Sigma-Aldrich, USA) and their locomotor activity was recorded for another 90 min.

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