Gw2974

Manufactured by Merck Group

GW2974 is a laboratory equipment product manufactured by Merck Group. It is designed for use in scientific research and analysis.

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Lab products found in correlation

Glibenclamide, by Bio-Techne (1 mentions) Gefitinib, by Selleck Chemicals (1 mentions) Ag1478, by Merck Group (1 mentions) Pd153035, by Bio-Techne (1 mentions) Marimastat, by Bio-Techne (1 mentions) Apc anti human egfr antibody, by BioLegend (1 mentions) Bapta am, by Bio-Techne (1 mentions) Zymosan a, by Merck Group (1 mentions) β actin a5441, by Merck Group (1 mentions) Deionized water, by Merck Group (1 mentions) Sch 442416, by Merck Group (1 mentions) Tris hydroxymethyl aminomethane hydrochloride tris hcl, by Merck Group (1 mentions)

4 protocols using gw2974

EGFR Kinase Inhibitors Modulate C. albicans Infection

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The EGFR kinase domain inhibitors were purchased from Santa Cruz (Gefitinib), Selleckchem (PD153035), Tocris Bioscience (AG1478) and Sigma (GW2974). Marimastat, GI-253023X, glibenclamide and Bapta-AM were purchased from Tocris Bioscience. Inhibitors were reconstituted in DMSO and aliquoted for appropriate freezer temperature storage. TR146 cells were incubated with inhibitors for 1 h prior to C. albicans infection, candidalysin exposure or mock treatment. Phospho-EGFR Tyr1068 (#3777), phospho-EGFR Tyr845 (#6963S), c-Fos (#2250S) and phospho-MKP1 (#2857S) antibodies were purchased from Cell Signalling Technology. Mouse anti-human α-actin antibody was purchased from Millipore (UK) (#MAB1501), goat anti-mouse (#115-035-062) and anti-rabbit (#111-035-144) horseradish peroxidase (HRP)-conjugated antibodies were purchased from Jackson Immunologicals (Stratech Scientific, UK). Fluorescent ErbB receptor affibodies were a kind gift from the Science and Technologies Facilities Council for use with confocal imaging work. APC anti-human EGFR antibody was purchased from BioLegend (# 352905) for use with Imagestream analyses. Biologically active EREG and EPG were purchased from Peprotech and used at 10, 25 and 50 ng/mL either individually or together at the same concentration.

Ho J., Yang X., Nikou S.A., Kichik N., Donkin A., Ponde N.O., Richardson J.P., Gratacap R.L., Archambault L.S., Zwirner C.P., Murciano C., Henley-Smith R., Thavaraj S., Tynan C.J., Gaffen S.L., Hube B., Wheeler R.T., Moyes D.L, & Naglik J.R. (2019). Candidalysin activates innate epithelial immune responses via epidermal growth factor receptor. Nature Communications, 10, 2297.

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Comprehensive Analysis of NF-κB Pathway

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Amphotericin B was purchased from X-GEN Pharmaceuticals Inc. Zymosan A was purchased from Sigma-Aldrich (Z4250-250 mg) and was boiled for one hour before use. The YPD agar medium was purchased from Clontech (Clontech, 630410). GW2974 was purchased from Sigma (G0668-50MG). The following antibodies were used for Western blotting and immunohistochemistry: IKKα (IMG-136A) and IκBα (IMG-127A) from IMAGENEX; IKKβ (05-535) from Millipore; lamin B (sc-6216), RelB (sc-48366), LTβR (sc-80167), p65 (sc-372), IκBα (sc-371), p50 (sc-1190), p16 (sc-377412), p53 (sc-6243), p63 (sc-8431), EGFR (sc-03), and IKKα (sc-7183) from Santa Cruz Biotechnology; Phospho-EGFR (2235), p65 (8242) and p100/p52 (4882) from Cell Signaling Technology; β-actin (A-5441) from Sigma; anti-alpha tubulin antibody (ab4074) from abcam; AIRE Alexa Fluor 488 (53-5934-80) and F4/80 (14-4801-81) from eBioscience; CD4 (550280) and IKKγ (559675) from BD Pharmingen; and biotinylated UEA-1 (B-1065) from Vector Laboratories. The anti-CD40 antibody (rat anti-mCD40 agonist, clone Fgk115) was kindly provided by Dr. Dr. Giorgio Trinchieri’s laboratory.

Zhu F., Willette-Brown J., Song N.Y., Lomada D., Song Y., Xue L., Gray Z., Zhao Z., Davis S.R., Sun Z., Zhang P., Wu X., Zhan Q., Richie E.R, & Hu Y. (2017). Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis. Cell host & microbe, 21(4), 478-493.e7.

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Aspirin and EGFR Inhibitor Effects on Ikkα Mice

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Five-month-old Ikkα^KA/KA mice were treated with either aspirin water solution or EGFR inhibitor GW2974 suspension added into wet feed food. Each mouse was given 3 mg of aspirin, or 0.6mg of GW2974 per day for one month. Aspirin (A5376-100G: Acetylsalicylic acid) and GW 2974 (G0668) were purchased from Sigma. Mice were singly housed to ensure full consumption of aspirin or GW2974.

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Telomeric DNA Binding Assay

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All chemicals were of the highest purity available (98%-99%) and used without further purification. GW-2974 (N4-(1-benzyl-1H-indazol-5-yl)-N6,N6-dimethyl-pyrido-[3,4-d]-pyrimidine-4,6-diamine), SCH-442416 (5-amino-7-[3-(4-methoxy) phenylpropyl]-2-(2-furyl)-pyrazolo[4,3-e]-1,2,4-triazolo[1,5-c]pyrimidine), ethylenediaminetetraacetic acid (EDTA), potassium chloride, Tris(hydroxymethyl)-aminomethane hydrochloride (Tris–HCl) and calf thymus DNA (c-DNA) were purchased from Sigma-Aldrich. High-performance liquid chromatography (HPLC)-purified telomeric DNA sequences, 5′-AGGGTTAGGGTTAGGGTTAGGG-3′, Fl-5′-AGGGTTAGGGTTAGGGTTAG-GG-3′ and 3′-TCCCAATCCCAATCCCAATCCC -5′, were purchased from Alpha DNA, Canada. Notably, Millipore deionized water was used in all experiments.

Salem A.A., El Haty I.A, & Ghattas M.A. (2022). GW-2974 and SCH-442416 modulators of tyrosine kinase and adenosine receptors can also stabilize human telomeric G-quadruplex DNA. PLOS ONE, 17(12), e0277963.

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