Matrigel matrix hc

Manufactured by Corning

Sourced in United States

Matrigel Matrix HC is a gelatinous protein mixture extracted from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is designed to mimic the extracellular matrix environment and supports the attachment, migration, proliferation, and differentiation of various cell types.

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Lab products found in correlation

Athymic nude mice, by Jackson ImmunoResearch (1 mentions) Penicillin streptomycin, by Corning (1 mentions) Male balb c mice, by Charles River Laboratories (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Matrigel (8653 citations) Matrigel matrix (1030 citations) Matrigel HC (17 citations) Matrigel Matrix High Concentration (HC (3 citations) Matrigel Membrane Matrix HC (2 citations)

3 protocols using matrigel matrix hc

Melanoma Xenograft Tumor Induction

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Infected melanoma cells were intradermally injected in quadruplicate into NOD.Cg‐Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice. We used 400,000, 600,000, and 200,000 cells/mouse for WM1552C, WM902B and MM13 PDX cells, respectively, resuspended in a 3:1 mix of L15 medium and Matrigel Matrix HC (Corning, Cat # 354248). The experiments were performed in duplicate, using four mice per group (shSCR/shTINCR or Empty vector/TINCR) per experiment. Metastasis formation was evaluated in MM13 transplanted mice, 4 weeks after the resection of primary tumors. Mice were sacrificed and metastases at lymph nodes, lungs and liver collected.

Melixetian M., Bossi D., Mihailovich M., Punzi S., Barozzi I., Marocchi F., Cuomo A., Bonaldi T., Testa G., Marine J., Leucci E., Minucci S., Pelicci P.G, & Lanfrancone L. (2021). Long non‐coding RNA TINCR suppresses metastatic melanoma dissemination by preventing ATF4 translation. EMBO Reports, 22(3), e50852.

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HepG2 Xenograft Tumor Establishment

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All in vivo procedures were approved and overseen by the Johns Hopkins Institutional Animal Care and Use Committee (IACUC). To establish xenograft tumors, we resuspended 1 million HepG2 cells in 100 μL of 50% Matrigel matrix HC (Corning, Corning, NY, USA) and 50% HBSS. Cells were injected subcutaneously in the hind flank of female 6- to 8-week-old athymic nude mice (The Jackson Laboratory, Bar Harbor, ME, USA). During implantation, animals were anesthetized with 2.5% isoflurane in oxygen. Tumors developed in ∼80% of mice after 14 days.

Vaughan H.J., Zamboni C.G., Radant N.P., Bhardwaj P., Revai Lechtich E., Hassan L.F., Shah K, & Green J.J. (2021). Poly(beta-amino ester) nanoparticles enable tumor-specific TRAIL secretion and a bystander effect to treat liver cancer. Molecular Therapy Oncolytics, 21, 377-388.

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CT26 Tumor Inoculation and Growth Monitoring

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CT26 cells were cultured in a sterile environment at 37 °C and 5% CO₂ using RPMI-1640 with Glutamax (Gibco) containing 10% FBS (Hyclone, SH30910.03, v/v) and 1% penicillin/streptomycin (Corning, 30–002-CI, v/v). All animal handling and experiments were conducted in accordance with the guidelines and regulations set by the Wayne State University Institutional Animal Care and Use Committee (IACUC). Male BALB/c mice (6–8 weeks, Charles River) were inoculated subcutaneously on the right shoulder with 1 × 10⁵ CT26 tumor cells in 1:1 RPMI-1640 medium: Matrigel Matrix HC (Corning, 354262). The tumor growth was monitored by caliper measurement with tumor volume calculated as V = L × W × H × π/6 until tumors reached a volume of ~150–250 mm³.

White J.M., Keinänen O.M., Cook B.E., Zeglis B.M., Gibson H.M, & Viola N.T. (2020). Removal of Fc Glycans from [89Zr]Zr-DFO-Anti-CD8 Prevents Peripheral Depletion of CD8+ T Cells. Molecular pharmaceutics, 17(6), 2099-2108.

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