Cell morphology was examined by fluorescence staining. After being cocultured with the composite for 4, 24, or 72 hours, the cell samples were fixed with 4% polyformaldehyde (Boster, Wuhan, People’s Republic of China) incubated for 1 hour with fluorescein isothiocyanate-phalloidin (Actin-Tracker Green Kit, Beyotime, Jiangsu, People’s Republic of China) in the dark and then incubated for 5 minutes with 2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride (Sigma-Aldrich, St Louis, MO, USA). Finally, the cytoskel-eton was observed under laser confocal microscopy (FV1000, Olympus, Tokyo, Japan).
Actin tracker green kit
Manufactured by Beyotime
Sourced in China
The Actin-Tracker Green Kit is a fluorescent labeling tool used to visualize and track actin filaments in live cells. It provides a simple and efficient method for labeling actin structures without the need for genetic modifications.
Automatically generated - may contain errors
2 protocols using actin tracker green kit
1
Cell Morphology Examination via Fluorescence
2
F-actin Visualization in PM2.5-treated A549 Cells
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F-actin in A549 cells was stained using the Actin-Tracker Green Kit (Beyotime, China), according to the manufacturer's protocol. Briefly, A549 cells were seeded in a 6-well plate and treated with PBS, 100 μg/mL or 500 μg/mL PM2.5, with or without miR-802 mimic, miR-1322 mimic, miR-802 and miR-1322 mimic rescues for 24h in 6-well plates, and then fixed in 4% PFA for 10 min. The Actin-Tracker Green was diluted by 1:80 with 0.1% Triton X-100/1% BSA/PBS, and 200 μL working solution was applied to each well for 20 min at room temperature. After washing with 0.1% Triton X-100/PBS three times, the actin morphology was observed under a fluorescent microscope (Olympus, Japan).
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